Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,185,239 C→A 12.9% P63Q (CCG→CAG)  PP_4558 → GNAT family acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,185,2390CA12.9% 97.6 / 3.2 31P63Q (CCG→CAG) PP_4558GNAT family acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (16/11);  new base A (2/2);  total (18/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

AGCACAGCTCACGCATGCGCGCTGCAAGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTGGCGCAGGTTGGCGGGCCCGTCTGGCTGTTCTGTCACCCCGACCTGATGCCCTTCTACCAGCGCCTGGGTTTCGACCTGGCCCAACA  >  NC_002947/5185110‑5185374
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cgCACAGCTCACGCATGCGCGCTGCAAGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAgcg                                                                                                                                   <  1:57154/135‑1 (MQ=255)
   acaGCTCACGCATGCGCGCTGCAAGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAgcggcg                                                                                                                                >  2:486618/1‑136 (MQ=255)
   acaGCTCACGCATGCGCGCTGCAAGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAgcggcg                                                                                                                                >  1:510512/1‑136 (MQ=255)
          aCGCATGCGCGCTGCAAGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCaa                                                                                                                         >  1:212368/1‑136 (MQ=255)
          aCGCATGCGCGCTGCAAGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCaa                                                                                                                         >  2:528488/1‑136 (MQ=255)
          aCGCATGCGCGCTGCAAGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTACGTCGCCCCGCAGCGGCGCAACCaa                                                                                                                         >  2:106539/1‑136 (MQ=255)
                         aaGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGgcgc                                                                                                                               >  1:170863/1‑115 (MQ=255)
                         aaGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGgcgc                                                                                                                               <  2:170863/115‑1 (MQ=255)
                         aaGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGa                                                                                                          >  2:433684/1‑136 (MQ=255)
                         aaGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGa                                                                                                          >  2:574246/1‑136 (MQ=255)
                         aaGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGa                                                                                                          >  1:7485/1‑136 (MQ=255)
                         aaGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGa                                                                                                          >  2:571090/1‑136 (MQ=255)
                         aaGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCAGCAGCGGCGCAACCAAGGGGTGGCTGCGCGa                                                                                                          >  2:418714/1‑136 (MQ=255)
                         aaGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCAGCAGCGGCGCAACCAAGGGGTGGCTGCGCGa                                                                                                          >  2:243642/1‑136 (MQ=255)
                                         ggggTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCgcgc                                                                                          <  2:53264/135‑1 (MQ=255)
                                                   gggCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTg                                                                                        <  1:246217/128‑1 (MQ=255)
                                                   gggCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTg                                                                                        >  2:246217/1‑128 (MQ=255)
                                                    ggCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTGGCGCAGGtt                                                                               >  1:68466/1‑136 (MQ=255)
                                                        ccTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCaa                                                                                                                             >  2:601959/1‑86 (MQ=255)
                                                        ccTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCaa                                                                                                                             <  1:601959/86‑1 (MQ=255)
                                                        ccTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCaa                                                                                                                             <  1:598663/86‑1 (MQ=255)
                                                        ccTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCaa                                                                                                                             >  2:598663/1‑86 (MQ=255)
                                                                    gCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTGGCGCAGGTTGGCGGGCCCGTCTGGc                                                               >  1:480116/1‑136 (MQ=255)
                                                                                cTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTGGCGCAGGTTGGCGGGCCCGTCTGGCTGTTCTGTCAcc                                                   >  2:6473/1‑136 (MQ=255)
                                                                                           tGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTGGCGCAGGTTGGCGGGCCCGTCTGGCTGTTCTGTCACCCCGACCTGATg                                        <  2:228759/136‑1 (MQ=255)
                                                                                                        tGGCTGACGGGCCTGTTCGTCGCCGAGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTGGCGCAGGTTGGCGGGCCCGTCTGGCTGTTCTGTCACCCCGACCTGATGCCCTTCTACCAgc                           <  1:243642/136‑1 (MQ=255)
                                                                                                        tGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTGGCGCAGGTTGGCGGGCCCGTCTGGCTGTTCTGTCACCCCGACCTGATGCCCTTCTACCAgc                           <  1:574246/136‑1 (MQ=255)
                                                                                                        tGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTGGCGCAGGTTGGCGGGCCCGTCTGGCTGTTCTGTCACCCCGACCTGATGCCCTTCTACCAgc                           <  1:571090/136‑1 (MQ=255)
                                                                                                        tGGCTGACGGGCCTGTTCGTCGCCCAGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTGGCGCAGGTTGGCGGGCCCGTCTGGCTGTTCTGTCACCCCGACCTGATGCCCTTCTACCAgc                           <  1:418714/136‑1 (MQ=255)
                                                                                                              aCGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTGGCGCAGGTTGGCGGGCCCGTCTGGCTGTTCTGTCACCCCGACCTGATGCCCTTCTACCAGCGCCTgg                     <  2:25679/136‑1 (MQ=255)
                                                                                                              aCGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTGGCGCAGGTTGGCGGGCCCGTCTGGCTGTTCTGTCACCCCGACCTGATGCCCTTCTACCAGCGCCTgg                     <  2:54583/136‑1 (MQ=255)
                                                                                                                              ccccGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTGGCGCAGGTTGGCGGGCCCGTCTGGCTGTTCTGTCACCCCGACCTGATGCCCTTCTACCAGCGCCTGGGTTTCGACCTGGCCCa     <  2:4544/136‑1 (MQ=255)
                                                                                                                                 cGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTGGCGCAGGTTGGCGGGCCCGTCTGGCTGTTCTGTCACCCCGACCTGATGCCCTTCTACCAGCGCCTGGGTTTCGACCTGGCCCAACa  <  2:480116/136‑1 (MQ=255)
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AGCACAGCTCACGCATGCGCGCTGCAAGCGACGCCGAACTGTGGGTAGCACGGGCCCCTGGCATCATCGCCGGGCTGAGCCTGAGTGCGGTTGGTGAGGGATTCTGGCTGACGGGCCTGTTCGTCGCCCCGCAGCGGCGCAACCAAGGGGTGGCTGCGCGATTGGTCGAGGCCGCGCTGGCGCAGGTTGGCGGGCCCGTCTGGCTGTTCTGTCACCCCGACCTGATGCCCTTCTACCAGCGCCTGGGTTTCGACCTGGCCCAACA  >  NC_002947/5185110‑5185374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑