| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,197,098 | C→A | 22.2% | R186S (CGC→AGC) | PP_4572 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,197,098 | 0 | C | A | 22.2% | 43.6 / 4.2 | 18 | R186S (CGC→AGC) | PP_4572 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (9/5); new base A (2/2); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GAAGTGCGTATCGACAATGGCGTGCTGACGTTCCGCAACTTCAATTCCAAGCCACCGGTCGACCTCAAGGCCACCCGGTTGAACGCCAGCATCCGCAACTTGACCAACGTGCGCGACCAGCAAGGCCGTCGCGATGCAAGTTTCGAAGGGACGGCGCTCATCGCGGGGGATGCGAAGGTAGAAAGCCGTGCCACCTTCGACCCTTTCAGTGATTTCGACGACTTCGAATTCAGGCTTCGCGCCACCGGCATCGAGCT > NC_002947/5196969‑5197225 | gAAGTGCGTATCGACAATGGCGTGCTGACGTTCCGCAACTTCAATTCCAAGCCACCGGTCGACCTCAAGGCCACCCGGTTGAACGCCAGCATCCGCAACTTGACCAACGTGCGCGACCAGCAAGGCCGTCGCGATg < 2:396269/136‑1 (MQ=255) cGTATCGACAATGGCGTGCTGACGTTCCGCAACTTCAATTCCAAGCCACCGGTCGACCTCAAGGCCACCCGGTTGAACGCCAGCATCCGCAACTTGACCAACGTGCGCGACCAGCAAGGCCGTAGCGATGCAAGtt > 2:73760/1‑136 (MQ=255) cGTATCGACAATGGCGTGCTGACGTTCCGCAACTTCAATTCCAAGCCACCGGTCGACCTCAAGGCCACCCGGTTGAACGCCAGCATCCGCAACTTGACCAACGTGCGCGACCAGCAAGGCCGTAGCGATGCAAGtt > 2:49411/1‑136 (MQ=255) aTCGACAATGGCGTGCTGACGTTCCGCAACTTCAATTCCAAGCCACCGGTCGACCTCAAGGCCACCCGGTTGAACGCCAGCATCCGCAACTTGACCAACGTGCGCGACCAGCAAGGCCGTCGCGATGCAAGTTTCg > 1:334582/1‑136 (MQ=255) aTCGACAATGGCGTGCTGACGTTCCGCAACTTCAATTCCAAGCCACCGGTCGACCTCAAGGCCACCCGGTTGAACGCCAGCATCCGCAACTTGACCAACGTGCGCGACCAGCAAGGCCGTCGCGATGCAAGTTTCg > 2:124114/1‑136 (MQ=255) gACGTTCCGCAACTTCAATTCCAAGCCACCGGTCGACCTCAAGGCCACCCGGTTGAACGCCAGCATCCGCAACTTGACCAACGTGCGCGACCAGCAAGGCCGTCGCGATGCAAGTTTCGAAGGGACGGCGCTCATc < 2:334582/136‑1 (MQ=255) aCGTTCCGCAACTTCAATTCCAAGCCACCGGTCGACCTCAAGGCCACCCGGTTGAACGCCAGCATCCGCAACTTGACCAACGTGCGCGACCAGCAAGGCCGTCGCGATGCAAGTTTCGAAGGGACGGCGCTCATcg > 1:540595/1‑136 (MQ=255) aaCTTCAATTCCAAGCCACCGGTCGACCTCAAGGCCACCCGGTTGAACGCCAGCATCCGCAACTTGACCAACGTGCGCGACCAGCAAGGCCGTCGCGATGCAAGTTTCGAAGGGACGGCGCTCATCGCGGGGGATg < 1:640360/136‑1 (MQ=255) tCCAAGCCACCGGTCGACCTCAAGGCCACCCGGTTGAACGCCAGCATCCGCAACTTGACCAACGTGCGCGACCAGCAAGGCCGTCGCGATGCAAGTTTCGAAGGGACGGCGCTCATCGCGGGGGATGCGAAGGTAg < 1:567022/136‑1 (MQ=255) cACCCGGTTGAACGCCAGCATCCGCAACTTGACCAACGTGCGCGACCAGCAAGGCCGTCGCGATGCAAGTTTCGAAGGGACGGCGCTCATCGCGGGGGATGCGAAGGTAGAAAGCCGTGCCACCTTCGACCCTTTc > 2:419795/1‑136 (MQ=255) gTTGAACGCCAGCATCCGCAACTTGACCAACGTGCGCGACCAGCAAGGCCGTCGCGATGCAAGTTTCGAAGGGACGGCGCTCATCGCGGGGGATGCGAAGGTAGAAAGCCGTGCCACCTTCGACCCTTTCAGTGAt > 2:555342/1‑136 (MQ=255) gTTGAACGCCAGCATCCGCAACTTGACCAACGTGCGCGACCAGCAAGGCCGTCGCGATGCAAGTTTCGAAGGGACGGCGCTCATCGCGGGGGATGCGAAGGTAGAAAGCCGTGCCACCTTCGACCCTTTCAGTGAt > 2:558473/1‑136 (MQ=255) aaCGCCAGCATCCGCAACTTGACCAACGTGCGCGACCAGCAAGGCCGTCGCGATGCAAGTTTCGAAGGGACGGCGCTCATCGCGGGGGATGCGAAGGTAGAAAGCCGTGCCACCTTCGACCCTTTCAGTGATTTcg > 1:38667/1‑136 (MQ=255) gcgACCAGCAAGGCCGTCGCGATGCAAGTTTCGAAGGGACGGCGCTCATCGCGGGGGATGCGAAGGTAGAAAGCCGTGCCACCTTCGACCCTTTCAGTGATTTCGACGACTTCGAATTCAGGCTTCGCGCCACCgg > 1:674306/1‑136 (MQ=255) gcgACCAGCAAGGCCGTCGCGATGCAAGTTTCGAAGGGACGGCGCTCATCGCGGGGGATGCGAAGGTAGAAAGCCGTGCCACCTTCGACCCTTTCAGTGATTTCGACGACTTCGAATTCAGGCTTCGCGCCACCgg > 1:21102/1‑136 (MQ=255) cAGCAAGGCCGTAGCGATGCAAGTTTCGAAGGGACGGCGCTCATCGCGGGGGATGCGAAGGTAGAAAGCCGTGCCACCTTCGACCCTTTCAGTGATTTAGACGACTTCGAATTAAGGCTTCGCGCCACCGGCATCg < 1:73760/136‑1 (MQ=255) cAGCAAGGCCGTAGCGATGCAAGTTTCGAAGGGACGGCGCTCATCGCGGGGGATGCGAAGGTAGAAAGCCGTGCCACCTTCGACCCTTTCAGTGATTTAGACGACTTCGAATTAAGGCTTCGCGCCACCGGCATCg < 1:49411/136‑1 (MQ=255) aaGGCCGTCGCGATGCAAGTTTCGAAGGGACGGCGCTCATCGCGGGGGATGCGAAGGTAGAAAGCCGTGCCACCTTCGACCCTTTCAGTGATTTCGACGACTTCGAATTCAGGCTTCGCGCCACCGGCATCGAGCt < 1:504595/136‑1 (MQ=255) | GAAGTGCGTATCGACAATGGCGTGCTGACGTTCCGCAACTTCAATTCCAAGCCACCGGTCGACCTCAAGGCCACCCGGTTGAACGCCAGCATCCGCAACTTGACCAACGTGCGCGACCAGCAAGGCCGTCGCGATGCAAGTTTCGAAGGGACGGCGCTCATCGCGGGGGATGCGAAGGTAGAAAGCCGTGCCACCTTCGACCCTTTCAGTGATTTCGACGACTTCGAATTCAGGCTTCGCGCCACCGGCATCGAGCT > NC_002947/5196969‑5197225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |