Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,237,539 C→A 12.9% A52S (GCC→TCC)  PP_4610 ← PepSY‑associated TM helix domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,237,5390CA12.9% 96.3 / 3.3 31A52S (GCC→TCC) PP_4610PepSY‑associated TM helix domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (14/13);  new base A (2/2);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

CACTGGGGTCGCGGTACCCCACACTCAAGGCGGCCTCCCGTTCGTTCGGCATGCGAATGAACCAGGTACTCGAATGCCCTGCATTGTCCTGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAGGTGTGCAGCCAGGCCATG  >  NC_002947/5237404‑5237669
                                                                                                                                       |                                                                                                                                  
cACTGGGGTCGCGGTACCCCACACTCAAGGCGGCCTCCCGTTCGTTCGGCATGCGAATGAACCAGGTACTCGAATGCCCTGCATTGTCCTGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGc                                                                                                                                    >  1:194023/1‑136 (MQ=255)
      ggTCGCGGTACCCCACACTCAAGGCGGCCTCCCGTTCGTTCGGCATGCGAATGAACCAGGTACTCGAATGCCCTGCATTGTCCTGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCt                                                                                                                              <  1:574914/136‑1 (MQ=255)
       gTCGCGGTACCCCACACTCAAGGCGGCCTCCCGTTCGTTCGGCATGCGAATGAACCAGGTACTCGAATGCCCTGCATTGTCCTGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTg                                                                                                                             <  1:546740/136‑1 (MQ=255)
                                     ccGTTCGTTCGGCATGCGAATGAACCAGGTACTCGAATGCCCTGCATTGTCCTGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGAATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGAtt                                                                                               >  2:667687/1‑136 (MQ=255)
                                     ccGTTCGTTCGGCATGCGAATGAACCAGGTACTCGAATGCCCTGCATTGTCCTGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGAATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGAtt                                                                                               >  2:177916/1‑136 (MQ=255)
                                         tcgttcgGCATGCGAATGAACCAGGTACTCGAATGCGCTGCATTGTCCTGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATttctt                                                                                           <  1:60096/136‑1 (MQ=255)
                                           gttcgGCATGCGAATGAACCAGGTACTCGAATGCCCTGCATTGTCCTGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTcc                                                                                         <  2:171380/136‑1 (MQ=255)
                                           gttcgGCATGCGAATGAACCAGGTACTCGAATGCCCTGCATTGTCCTGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTcc                                                                                         <  1:171838/136‑1 (MQ=255)
                                                                          tGCCCTGCATTGTCCTGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGa                                                          >  1:103442/1‑136 (MQ=255)
                                                                          tGCCCTGCATTGTCCTGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGa                                                          >  1:58846/1‑136 (MQ=255)
                                                                               tGCATTGTCCTGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCg                                                     >  1:499696/1‑136 (MQ=255)
                                                                                         tGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCg                                                                         <  2:655634/106‑1 (MQ=255)
                                                                                         tGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCg                                                                         >  1:655634/1‑106 (MQ=255)
                                                                                         tGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAAcagcc                                           >  2:35686/1‑136 (MQ=255)
                                                                                          gCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAAcagcca                                          >  1:59024/1‑136 (MQ=255)
                                                                                                aaCGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGaa                                    <  1:35686/136‑1 (MQ=255)
                                                                                                   gTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGag                                 >  2:623654/1‑135 (MQ=255)
                                                                                                   gTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGAt                                 >  1:430905/1‑136 (MQ=255)
                                                                                                   gTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGAt                                 >  2:519149/1‑136 (MQ=255)
                                                                                                   gTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGAt                                 >  2:436741/1‑136 (MQ=255)
                                                                                                   gTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACATCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGag                                 >  1:102833/1‑135 (MQ=255)
                                                                                                     tGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCa                               <  1:140683/136‑1 (MQ=255)
                                                                                                     tGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCa                               <  2:499696/136‑1 (MQ=255)
                                                                                                          gACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCAGGCCg                          <  2:58846/136‑1 (MQ=255)
                                                                                                                cAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAg                    >  2:128721/1‑136 (MQ=255)
                                                                                                                cAGGCTATTGATCGGGTCCAGGGAATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAg                    <  1:667687/136‑1 (MQ=255)
                                                                                                                cAGGCTATTGATCGGGTCCAGGGAATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAg                    <  1:177916/136‑1 (MQ=255)
                                                                                                                  ggCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATTGCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAGgt                  >  2:111008/1‑136 (MQ=255)
                                                                                                                      tttGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAGGTGTGc              <  1:425336/135‑1 (MQ=255)
                                                                                                                      aTTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAGGTGTGc              <  2:60850/136‑1 (MQ=255)
                                                                                                                            cGGGTCCAGTGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAGGTGTGCAGCCAg        <  1:105071/136‑1 (MQ=255)
                                                                                                                                  cAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGATCAACAGCCAGCCGAAGATCAGGCCGGTCCAGGTGTGCAGCCAGGCCATg  >  2:117568/1‑136 (MQ=255)
                                                                                                                                       |                                                                                                                                  
CACTGGGGTCGCGGTACCCCACACTCAAGGCGGCCTCCCGTTCGTTCGGCATGCGAATGAACCAGGTACTCGAATGCCCTGCATTGTCCTGCAGGTAACGTTGAGCGACCGCCAGGCTATTGATCGGGTCCAGGGCATGGCTGCGCACTTCAGGTTGCGACCAATGAGTGATTTCTTCCTTGAAGTACGACAGCGTGCCGGTAAGGAAGATGGCGAACAACAGCCAGCCGAAGATCAGGCCGGTCCAGGTGTGCAGCCAGGCCATG  >  NC_002947/5237404‑5237669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑