Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,432,753 G→T 13.7% P116Q (CCA→CAA)  rhlE‑I ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,432,7530GT13.7% 93.0 / 2.4 29P116Q (CCA→CAA) rhlE‑IATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base G (13/12);  new base T (2/2);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

TGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCT  >  NC_002947/5432622‑5432886
                                                                                                                                   |                                                                                                                                     
agCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGAtt                                                                                                                                   >  1:499663/2‑136 (MQ=255)
     tCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATAc                                                                                                                              <  2:630733/136‑1 (MQ=255)
     tCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATAc                                                                                                                              <  2:31905/136‑1 (MQ=255)
     tCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATAc                                                                                                                              >  1:510003/1‑136 (MQ=255)
     gcGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATAc                                                                                                                              <  1:663337/135‑1 (MQ=255)
     ccGGCTTCATCCAGCACCTGGTTCTGCACCTGGTTTAACTTCACGGCGTTCTGCCTGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTAATAc                                                                                                                              <  2:104668/135‑1 (MQ=255)
       ggCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTg                                                                                                                            <  2:157796/136‑1 (MQ=255)
       ggCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTg                                                                                                                            <  1:595707/136‑1 (MQ=255)
          ttCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTGAcc                                                                                                                         >  1:496852/1‑136 (MQ=255)
            cATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTGAcccc                                                                                                                       <  1:656423/136‑1 (MQ=255)
                    aCCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTGACCCCGCCATaca                                                                                                               <  2:510003/136‑1 (MQ=255)
                      cAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTGACCCCGCCATACAcc                                                                                                             >  2:20696/1‑136 (MQ=255)
                           tCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTGACCCCGCCATACACCGCGTa                                                                                                        >  2:317376/1‑136 (MQ=255)
                            cTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTGACACCGCCATACACCGCGTaa                                                                                                       >  1:115658/1‑136 (MQ=255)
                                      ttGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCg                                                                                             >  1:140676/1‑136 (MQ=255)
                                                 ggCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTTGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCg                                                                                  >  2:170578/1‑136 (MQ=255)
                                                 ggCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTTGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCg                                                                                  >  2:409928/1‑136 (MQ=255)
                                                 ggCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCg                                                                                  >  2:224237/1‑136 (MQ=255)
                                                             gAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCgcgc                                                                      <  2:496852/136‑1 (MQ=255)
                                                             gAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCgcgc                                                                      <  2:73396/136‑1 (MQ=255)
                                                                       aGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACTCCCCGTCGCAGTCGCATCATCTGTGGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTtgttgt                                                            <  2:245651/136‑1 (MQ=255)
                                                                                            accaGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTcgc                                       >  1:254608/1‑136 (MQ=255)
                                                                                              cagcagGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGa                                     <  1:533390/136‑1 (MQ=255)
                                                                                                 cagGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTAGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTg                                  >  1:526670/1‑136 (MQ=255)
                                                                                                                 cgcaggcgcaTCATCTGTTGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAgg                  <  1:409928/136‑1 (MQ=255)
                                                                                                                 cgcaggcgcaTCATCTGTTGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAgg                  <  1:170578/136‑1 (MQ=255)
                                                                                                                 cgcaggcgcaTCATCTGTGGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAgg                  >  1:146372/1‑136 (MQ=255)
                                                                                                                         catcatCTGTGGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGCTc          >  1:116644/1‑136 (MQ=255)
                                                                                                                         catcatCTGTGGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATc          >  1:113507/1‑136 (MQ=255)
                                                                                                                                 gtgGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCt  <  2:499663/136‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                     
TGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCAGCAGGTCGACACCCCGGCGCAGGCGCATCATCTGTGGATTGATACTGACCCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCT  >  NC_002947/5432622‑5432886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑