Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,787,235	C→A	19.1%	I235I (ATC→ATA)	kefA →	intermediate mechanosensitive channel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,787,235	0	C	A	19.1%	48.2 / 4.1	21	I235I (ATC→ATA)	kefA	intermediate mechanosensitive channel protein
Reads supporting (aligned to +/- strand):  ref base C (11/5);  new base A (2/2);  total (13/8)
Fisher's exact test for biased strand distribution p-value = 5.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

CCGAGTTGGCCTCGCTCAACGCGCTGACCCTGCTGCGCCGCCAGGAACTGGCCGGCAACAGCCTGCTGCAAGACCTCGGCAATGCGCGTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACGCTGATCAACGCCAAACGCCTGGCCCAGTCCCAGGAAGCCGTCACCCAGCAGTCGATCGAGGCTCAGAAGGCCGGCGGCAGCAGCCTGCTGGCCACTGAAAGCGC  >  NC_002947/5787102‑5787354                                                                                                                                      |                                                                                                                        ccGAGTTGGCCTCGCTCAACGCGCTGACCCTGCTGCGCCGCCAGGAACTGGCCGGCAACAGCCTGCTGCAAGACCTCGGCAATGCGCGTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCa                                                                                                                       <  1:205027/136‑1 (MQ=255)       tGGCCTCGCTCAACGCGCTGACCCTGCTGCGCCGCCAGGAACTGGCCGGCAACAGCCTGCTGCAAGACCTCGGCAATGCGCGTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCt                                                                                                                 >  1:428977/1‑136 (MQ=255)              gCTCAACGCGCTGACCCTGCTGCGCCGCCAGGAACTGGCCGGCAACAGCCTGCTGCAAGACCTCGGCAATGCGCGTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACg                                                                                                          >  1:332770/1‑136 (MQ=255)                 cAACGCGCTGACCCTGCTGCGCCGCCAGGAACTGGCCGGCAACAGCCTGCTGCAAGACCTCGGCAATGCGCGTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACGCTg                                                                                                       >  2:179265/1‑136 (MQ=255)                       gcTGACCCTGCTGCGCCGCCAGGAACTGGCCGGCAACAGCCTGCTGCAAGACCTCGGCAATGCGCGTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACGCTGATCAAc                                                                                                 >  2:61697/1‑136 (MQ=255)                              ctgctgCGCCGCCAGGAACTGGCCGGCAACAGCCTGCTGCAAGACCTCGGCAATGCGCGTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACGCTGATCAACGCCAAAc                                                                                          <  1:14626/136‑1 (MQ=255)                                   gcgcCGCCAGGAACTGGCCGGCAACAGCCTGCTGCAAGACCTCGGCAATGCGCGTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACGCTGATCAACGCCAAACGCCTg                                                                                     <  1:545290/136‑1 (MQ=255)                                             gAACTGGCCGGCAACAGCCTGCTGCAAGTCCTCGGCAATGCGCGTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACGCTGATCAACGCCAAACGCCTGGCCCAGTccc                                                                           <  1:179265/136‑1 (MQ=255)                                                           cAGCCTGCTGCAAGACCTCGGCAATGCGCGTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATACAGGACCTGCAGACGCTGATCAACGCCAAACGCCTGGCCCAGTCCCAGGAAGCCGTCAcc                                                             >  2:438567/1‑136 (MQ=255)                                                           cAGCCTGCTGCAAGACCTCGGCAATGCGCGTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATACAGGACCTGCAGACGCTGATCAACGCCAAACGCCTGGCCCAGTCCCAGGAAGCCGTCAcc                                                             >  2:379474/1‑136 (MQ=255)                                                                     cAAGACCTCGGCAATGCGCGTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACGCTGATCAACGCCAAACGCCTGGCCCAGTCCCAGGAAGCCGTCACCCAGCAGtcga                                                   <  2:615544/136‑1 (MQ=255)                                                                     cAAGACCTCGGCAATGCGCGTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACGCTGATCAACGCCAAACGCCTGGCCCAGTCCCAGGAAGCCGTCACCCAGCAGtcga                                                   >  1:660081/1‑136 (MQ=255)                                                                                  aTGCGCGTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACGCTGATCAACGCCAAACGCCTGGCCCAGTCCCAGGAAGCCGTCACCCAGCAGTCGATCGAGGCTCAGaa                                      >  1:69719/1‑136 (MQ=255)                                                                                      gcgTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACGCTGATCAACGCCAAACGCCTGGCCCAGTCCCAGGAAGCCGTCACCCAGCAGTCGATCGAGGCTCAGAAGGcc                                  >  1:152537/1‑136 (MQ=255)                                                                                        gTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACGCTGATCAACGCCAAACGCCTGGCCCAGTCCCAGGAAGCCGTCACCCAGCAGTCGATCGAGGCTCAGAAGGCcgg                                >  2:144357/1‑136 (MQ=255)                                                                                        gTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACGCTGATCAACGCCAAACGCCTGGCCCAGTCCCAGGAAGCCGTCACCCAGCAGTCGATCGAGGCTCAGAAGGCcgg                                >  2:640136/1‑136 (MQ=255)                                                                                           aCGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACGCTGATCAACGCCAAACGCCTGGCCCAGTCCCAGGAAGCCGTCACCCAGCAGTCGATCGAGGCTCAGAAGGCcggcgg                             >  2:667572/1‑136 (MQ=255)                                                                                                     ggTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACGCTGATCAACGCCAAACGCCTGGCCCAGTCCCAGGAAGCCGTCACCCAGCAGTCGATCGAGGCTCAGAAGGCCGGCGGCAGCAGCctg                   >  1:629014/1‑136 (MQ=255)                                                                                                                  gcgcgcCTGGAGCATGAAATGCAGGACCTGCAGACGCTGATCTAGCCCAATCGGGTGGCCCTGTCGCATGATGCCGTCTCCCAGCAGTCGATCGAGGCTCAGAAGGGCTGCGGCAGCTGCGTGCTGGCCACTGaaa      <  2:651178/136‑1 (MQ=255)                                                                                                                      gcCTGGAGCAGGAAATACAGGACCTGCAGACGCTGATCAACGCCAAACGCCTGGCCCAGTCCCAGGAAGCCGTCACCAAGCAGTCGATCGAGGCTCAGAAGGCCGGCGGCAGCAGCCTGCTGGCCACTGAAAgcgc  <  1:438567/136‑1 (MQ=255)                                                                                                                      gcCTGGAGCAGGAAATACAGGACCTGCAGACGCTGATCAACGCCAAACGCCTGGCCCAGTCCCAGGAAGCCGTCACCAAGCAGTCGATCGAGGCTCAGAAGGCCGGCGGCAGCAGCCTGCTGGCCACTGAAAgcgc  <  1:379474/136‑1 (MQ=255)                                                                                                                                      |                                                                                                                        CCGAGTTGGCCTCGCTCAACGCGCTGACCCTGCTGCGCCGCCAGGAACTGGCCGGCAACAGCCTGCTGCAAGACCTCGGCAATGCGCGTCACGACCTGATGGTCGAGCGCGCTGCGCGCCTGGAGCAGGAAATCCAGGACCTGCAGACGCTGATCAACGCCAAACGCCTGGCCCAGTCCCAGGAAGCCGTCACCCAGCAGTCGATCGAGGCTCAGAAGGCCGGCGGCAGCAGCCTGCTGGCCACTGAAAGCGC  >  NC_002947/5787102‑5787354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑