Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,075,201 T→G 18.9% N150T (AAC→ACC)  PP_5324 ← two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,075,2010TG18.9% 86.3 / 4.5 45N150T (AAC→ACC) PP_5324two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base T (9/27);  new base G (7/2);  total (16/29)
Fisher's exact test for biased strand distribution p-value = 5.68e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTACCTTGGTCAGCAGTTGCTCGTTGGTGAACGGCTT  >  NC_002947/6075074‑6075328
                                                                                                                               |                                                                                                                               
cAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTGGGCGCCGCCCCCGTGACCGCGTCGGGCGTGCCCGCAGGCAGCGCGGGCAACGAg                                                                                                                         >  1:186752/1‑136 (MQ=255)
     ggccctggccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGGGTCGGGGCCGCCCCCATGACCGCGTCGGGCTTGCCCGCAGGCCGCGCGGTCAACGAGTCGtt                                                                                                                    >  1:28915/1‑136 (MQ=255)
      gccgtggccACGGGCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGAGCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTg                                                                                                                   <  1:500704/136‑1 (MQ=255)
          tggccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCGTGCCCGCGGCGGGCTTGCCCGCAGGCAGCGCGGTCAACGAGGCGTTGGcga                                                                                                               >  1:211289/1‑134 (MQ=255)
               aCGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGcc                                                                                                          <  1:496050/136‑1 (MQ=255)
               aCGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGcc                                                                                                          <  1:458503/136‑1 (MQ=255)
                    cGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGGTGAACGAGTCGTTGGCAAAGGCCggggt                                                                                                     >  2:16388/1‑134 (MQ=255)
                    cGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGGGTCGGCGCCGCCCCCTTGGCCGCTTCGGGCGTGGCCGCCGGCAGCGCGGTCAACGCGTGGTTGGGCAAGGGCggggt                                                                                                     >  2:564570/1‑134 (MQ=255)
                              gggggCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTGGGCGCCGCCCCCATGGCCGCGGGGGGCTTGGCCGCAGTCAGCGCGGTAAACGCGGCGTTGGGAAAGGCCGGGGTGGGTCGCGcc                                                                                           >  2:139272/1‑136 (MQ=255)
                                  gcggcAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGc                                                                                       <  1:564570/136‑1 (MQ=255)
                                             gggCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATc                                                                            <  1:123885/136‑1 (MQ=255)
                                             gggCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATc                                                                            <  1:386582/136‑1 (MQ=255)
                                                  ccgcGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGGCCGCAGTCAGCGCGTTCACCGAGTCGTTGGCTAAGGCCGGGTTGGCTCGCGCCGGGCCGATGTCCATCAGGCt                                                                       <  1:582918/136‑1 (MQ=255)
                                                     cgACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTc                                                                    <  2:650011/136‑1 (MQ=255)
                                                          aaaGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCt                                                               <  1:21913/136‑1 (MQ=255)
                                                          aaaGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCt                                                               <  1:633895/136‑1 (MQ=255)
                                                          aaaGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCt                                                               <  2:670241/136‑1 (MQ=255)
                                                          aaaGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTGCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCTTGACGATGGCCATCAGGCTGTCGAGCt                                                               <  1:191507/136‑1 (MQ=255)
                                                                     cggtgccggtgTAGGCGCCGCCACCTTGACCGCTTAGGGCTTGCCCCCCGTCAGCGCGTTGAACGAGTCGTTGGGAAAGGCCGGGTTGGCTCGCGCCGGGCCGCTGGCCATCAGGCTGTCGAGCGTAACGAGCttt                                                    >  2:240775/1‑135 (MQ=255)
                                                                        tgccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTc                                                 <  1:37982/136‑1 (MQ=255)
                                                                         gccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCa                                                <  2:38536/136‑1 (MQ=255)
                                                                         gccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCa                                                <  1:3865/136‑1 (MQ=255)
                                                                         gccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCa                                                <  1:139272/136‑1 (MQ=255)
                                                                          gcggtgTCTGCGGCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGTGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAg                                               <  1:131996/135‑1 (MQ=255)
                                                                          ccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAg                                               <  1:167346/136‑1 (MQ=255)
                                                                          ccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAg                                               <  2:304008/136‑1 (MQ=255)
                                                                                  ggCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGctttct                                       <  1:395558/136‑1 (MQ=255)
                                                                                  ggCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGctttct                                       <  2:431847/136‑1 (MQ=255)
                                                                                    cgccgcCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGctttcttt                                     <  2:492660/136‑1 (MQ=255)
                                                                                     gccgccACCATGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGGTCAACGAGTCGTTGGGAAAGGCCCGGTTTGGTCGCGCCGGGCCGCTGGGCAGCACGGGGGGGAGCGTACCGATCTTGGTCAGGGcgttgtttt                                    >  2:236942/1‑135 (MQ=255)
                                                                                       ggccACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTAcc                                  <  1:341298/135‑1 (MQ=255)
                                                                                         ccACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTACCtt                                <  1:549332/136‑1 (MQ=255)
                                                                                            ccTTGTCCGGCTCGGGGTTGCCCGCAGGCAGCGCGGTCCACGTGGCGGTGGCAAAGGCCGGGGTGGCGCGCGGCGGGGCGTTTGCCATCAGGCTGGCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTACCTTGGt                             <  1:29101/136‑1 (MQ=255)
                                                                                            ccTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTACCTTGGt                             <  2:158588/136‑1 (MQ=255)
                                                                                            ccTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTACCTTGGt                             >  1:313354/1‑136 (MQ=255)
                                                                                                    gCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTACCTTGGTCAGCAGtt                     <  1:434361/136‑1 (MQ=255)
                                                                                                      gtCGGGCGTGCCCGCAGTCAGCGCGGTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTACCTTGGTCAGCAGTTGc                   <  1:315979/135‑1 (MQ=255)
                                                                                                         gggCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTACCTTGGTCAGCAGTTGCTCg                >  2:371284/1‑136 (MQ=255)
                                                                                                         gggCTAGCCAGCAGTCAGCGCGTTCAACGAGACGATGCCAAACGCCGGGTTGGCTCGAGCCGGCCCGATCGCCATCACGCTGACGAGCTTACAGAACTTCGTCAGGGCATTCATTACCTACGTCAGCAGATGcacc                >  2:385248/1‑133 (MQ=255)
                                                                                                          ggCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTACCTTGGTCAGCAGTTGCTCGt               >  1:602022/1‑136 (MQ=255)
                                                                                                           gCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTACCTTGGTCAGCAGTTGCTCGtt              >  1:185408/1‑136 (MQ=255)
                                                                                                                  cGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCTGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTACCTTGGTCAGCAGTTGCTCGTTGGTGaaa       >  2:311714/1‑135 (MQ=255)
                                                                                                                   gCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTTCCGATCTTGGTCAGGGCTTTCTTTACCTTGGTCAGCAGTTGCTCGTTGGTGAACg      >  2:625303/1‑136 (MQ=255)
                                                                                                                   gCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTACCTTGGTCAGCAGTTGCTCGTTGGTGATCg      >  2:595766/1‑136 (MQ=255)
                                                                                                                       tCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTACCTTGGTCAGCAGTTGCTCGTTGGTGAACGGCtt  <  2:492380/136‑1 (MQ=255)
                                                                                                                               |                                                                                                                               
CAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTACCTTGGTCAGCAGTTGCTCGTTGGTGAACGGCTT  >  NC_002947/6075074‑6075328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑