Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 146,084 G→A 12.9% A440T (GCC→ACC)  gltP → glutamate/aspartate‑proton DAACS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947146,0840GA12.9% 80.7 / 4.1 31A440T (GCC→ACC) gltPglutamate/aspartate‑proton DAACS transporter
Reads supporting (aligned to +/- strand):  ref base G (12/15);  new base A (2/2);  total (14/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.54e-01

TGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGG  >  NC_002947/145950‑146218
                                                                                                                                      |                                                                                                                                      
tGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGCGAGCTGTAGTGGTTGGCGAAACCGc                                                                                                                                       >  1:412235/1‑136 (MQ=255)
     gTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAgc                                                                                                                                  >  2:24520/1‑136 (MQ=255)
     gTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAgc                                                                                                                                  >  2:552256/1‑136 (MQ=255)
     gTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAgc                                                                                                                                  <  1:455538/136‑1 (MQ=255)
     gTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAgc                                                                                                                                  <  1:404554/136‑1 (MQ=255)
     gTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAgc                                                                                                                                  >  1:43898/1‑136 (MQ=255)
          gggCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGa                                                                                                                             >  1:345463/1‑136 (MQ=255)
               aCGCCTTTGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTg                                                                                                                        >  2:386262/1‑136 (MQ=255)
                       gCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTAGGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGt                                                                                                                <  1:152935/136‑1 (MQ=255)
                             ttGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCACCAAGCGCTGAGCCTGATGGCTGTTTGAcc                                                                                                          >  1:2286/1‑136 (MQ=255)
                             ttGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCACCAAGCGCTGAGCCTGATGGCTGTTTGAcc                                                                                                          >  1:453451/1‑136 (MQ=255)
                                             gggAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGt                                                                                          >  2:256863/1‑136 (MQ=255)
                                                aaGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCgg                                                                                       <  2:575413/136‑1 (MQ=255)
                                                        tACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCttttt                                                                               <  2:345463/136‑1 (MQ=255)
                                                          cgacgCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGt                                                                             <  2:43898/136‑1 (MQ=255)
                                                               cgGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCt                                                                        <  1:468698/136‑1 (MQ=255)
                                                                ggTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTg                                                                       >  1:473023/1‑136 (MQ=255)
                                                                              aGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGcc                                                         >  2:28285/1‑136 (MQ=255)
                                                                                           gCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAgg                                            >  2:519112/1‑136 (MQ=255)
                                                                                             tGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCt                                          >  1:241020/1‑136 (MQ=255)
                                                                                                   ccGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGAt                                    <  1:371612/136‑1 (MQ=255)
                                                                                                   ccGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGAt                                    <  1:1860/136‑1 (MQ=255)
                                                                                                   ccGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCACCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGAt                                    <  2:2286/136‑1 (MQ=255)
                                                                                                   ccGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCACCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGAt                                    <  2:453451/136‑1 (MQ=255)
                                                                                                                     tAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCGCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCg                  <  1:370963/136‑1 (MQ=255)
                                                                                                                     tAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCg                  <  1:256863/136‑1 (MQ=255)
                                                                                                                      aGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGa                 <  1:602890/136‑1 (MQ=255)
                                                                                                                                gAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCtatta       >  1:599715/1‑136 (MQ=255)
                                                                                                                                     cGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCgg  <  1:552256/136‑1 (MQ=255)
                                                                                                                                     cGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCgg  <  1:24520/136‑1 (MQ=255)
                                                                                                                                     cGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCgg  <  2:473023/136‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
TGAACGTTGTGGGCAACGCCTTGGCTGCATTGGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGG  >  NC_002947/145950‑146218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑