Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	527,937	A→T	19.2%	noncoding (1172/2902 nt)	PP_23SC →	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	527,937	0	A	T	19.2%	123.5 / 20.3	59	noncoding (1172/2902 nt)	PP_23SC	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (20/27);  new base T (3/9);  total (23/36)
Fisher's exact test for biased strand distribution p-value = 3.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

ACAGCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGAAAG  >  NC_002947/527804‑528058                                                                                                                                         |                                                                                                                          aCAGCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGatgatg                                                                                                                         <  1:419982/136‑1 (MQ=32)       acgatgTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCAACGGGTATCA‑TCTTA‑‑CGATGATGCGGTag                                                                                                                   >  1:460286/6‑136 (MQ=12)        ggatgTTGGCTTAGAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTaga                                                                                                                  >  1:642119/1‑136 (MQ=255)        ggatgTTGGCTTAGAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTaga                                                                                                                  >  1:627790/1‑136 (MQ=255)        ggatgTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTaga                                                                                                                  >  1:163685/1‑136 (MQ=17)        ggatgTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTaga                                                                                                                  >  1:226471/1‑136 (MQ=17)        ggaggTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTaga                                                                                                                  >  1:562375/1‑136 (MQ=32)              tGGCTTAGAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCg                                                                                                            >  1:117511/1‑136 (MQ=12)                  ttAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCt                                                                                                        >  1:135122/1‑136 (MQ=32)                     taaGCAGCCACCCTTTAAAGAAAGCGTATTAGCTCACTAGTCGAGTCGGGCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTa                                                                                                     <  1:79475/135‑1 (MQ=12)                       agcagcCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACAGGTATCA‑TCTTA‑‑CGATGATGCTGTACAGGATCGTTCTGTAAg                                                                                                   >  2:270513/1‑136 (MQ=255)                          agcCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCt                                                                                                >  1:273469/1‑136 (MQ=12)                              aCCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGa                                                                                            >  2:460001/1‑136 (MQ=32)                              aCCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGa                                                                                            >  2:57000/1‑136 (MQ=32)                                         aaagCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTgtgctg                                                                                  >  1:303388/1‑131 (MQ=0)                                          aagCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAATAATGTGAAGGTGAGTTgag                                                                                <  1:135315/136‑1 (MQ=12)                                              gTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGc                                                                            >  1:281630/1‑136 (MQ=32)                                                   aGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCt                                                                       <  2:117511/136‑1 (MQ=17)                                                   aGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCt                                                                       >  1:391247/1‑136 (MQ=32)                                                   aGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCt                                                                       <  2:579762/136‑1 (MQ=32)                                                      tCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTgg                                                                     >  1:551397/1‑136 (MQ=0)                                                                  tcgGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGt                                                        >  1:460044/1‑136 (MQ=32)                                                                  tcgGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAg                                                         >  2:650262/1‑136 (MQ=0)                                                                       cTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGaa                                                   >  2:354611/1‑136 (MQ=32)                                                                            cgGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTg                                              <  1:140452/136‑1 (MQ=32)                                                                              gAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                            <  2:661182/136‑1 (MQ=32)                                                                              gAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                            <  2:454076/136‑1 (MQ=32)                                                                               aaGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                            <  2:30331/136‑1 (MQ=0)                                                                                tgATGTAACGGGGCTCAAACCTTGCTCCGAAGGTACGGGTATGA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:627790/135‑1 (MQ=255)                                                                                aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCT‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:358307/136‑1 (MQ=17)                                                                                aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:511246/136‑1 (MQ=32)                                                                                aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:546473/136‑1 (MQ=32)                                                                                aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:416454/136‑1 (MQ=32)                                                                                aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:163685/136‑1 (MQ=32)                                                                                aGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACa                                           <  2:544602/136‑1 (MQ=0)                                                                                 gATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGTATGCTGACATg                                         <  2:415240/136‑1 (MQ=17)                                                                                 gATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATg                                         <  2:226471/136‑1 (MQ=32)                                                                                 gATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATg                                         <  2:562375/136‑1 (MQ=32)                                                                                 gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:623273/136‑1 (MQ=0)                                                                                 gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGATGTATCAGAAGTGCGAATGCTGACAt                                          <  2:24214/136‑1 (MQ=0)                                                                                 gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:21523/136‑1 (MQ=0)                                                                                 gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:231097/136‑1 (MQ=0)                                                                                  aTGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACAta                                         <  2:233666/136‑2 (MQ=0)                                                                                            gCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACaa                              <  2:339759/136‑1 (MQ=32)                                                                                              tCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATg                            >  1:262908/1‑136 (MQ=32)                                                                                               cAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGc                           >  2:262715/1‑136 (MQ=32)                                                                                                         aCCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGaaaaa                 >  1:480716/1‑136 (MQ=32)                                                                                                                 tACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACg         <  2:135122/136‑1 (MQ=32)                                                                                                                      ttATCA‑TTTTA‑‑CGATTATGCGGTTGTGTAGGTTTCTTTAAGCCTGTTATGGTGTGTTTTGAAGCTTGCTGGTGTTATCAGAAGTGCGAATGCTGACATTTGTAACGACAATGCGTGTGAAAAACTCGCACGCCGaa    <  2:460286/135‑1 (MQ=2)                                                                                                                      ttATCA‑TCTTA‑‑CTATTATGCGGTTGAGGAGGGTTCTGTATGCCTGTGAAGGTTTGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGGTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa    <  2:133794/135‑1 (MQ=11)                                                                                                                      ttATCA‑TCTTA‑‑CGATGATGCGGTAGAGGTGCGTTCTGTAAGCCTGTGATGGTGTGTTGAGAAGCTTGCTGTAGGTATCTGATGTGCGAATGCTGACATGTGTAACGACAATGCGTGTGAAAAACTCGCACGCCGaa    <  2:642119/135‑1 (MQ=11)                                                                                                                      gtttca‑tcTTA‑‑CGATGATGGGGTAGAGGAGGGTTGTGTAAGCCTGTGATGGTGTGTTGATAAGCTTGCTGGAGGTATCAGATGTGCTAATGCTGACATGAGTAACGACAATGCGTGTGAAAAACTCGCACGCCGaa    <  2:360482/133‑1 (MQ=11)                                                                                                                      gtttca‑taTTA‑‑CGATTTTGCGTTAGAGGAGCGTTCTGTAAGCCTGTGATGGTGAGTTGAGATGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa    <  2:486053/133‑1 (MQ=11)                                                                                                                      gTATCT‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTTTGAAGGTGTGTTGAGATGCTTGCGGGAGGTATCAGAAGTGCGAATGCTGACATGATTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa    <  2:455972/136‑1 (MQ=11)                                                                                                                      gTATCA‑TCTTA‑‑CGATGTGGCGGTAGAGGAGCGTTCTGTAAGCCTGTGATGGTGAGTTGAGAAGCTTGCTGGAGTTATCAGAAGTGCTAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa    <  2:404425/136‑1 (MQ=11)                                                                                                                      gTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa    <  2:496150/136‑1 (MQ=32)                                                                                                                      gTATCA‑TATTA‑‑CGTTTATGCGGTAGAGGAGCGTTCTTTAAGCCTGTTTAGGTTTGTTGATAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGTGTGAAAACCTCGCACGCCGaa    <  2:130845/136‑1 (MQ=11)                                                                                                                        atca‑tcTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGAAAg  <  2:435294/136‑1 (MQ=32)                                                                                                                                    cGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          >  1:647314/1‑87 (MQ=31)                                                                                                                                    cGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:647314/87‑1 (MQ=31)                                                                                                                                         |                                                                                                                          ACAGCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGAAAG  >  NC_002947/527804‑528058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑