Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 527,985 T→A 18.6% noncoding (1220/2902 nt) PP_23SC → 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947527,9850TA18.6% 87.0 / 17.6 43noncoding (1220/2902 nt)PP_23SC23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (7/28);  new base A (2/6);  total (9/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

AGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGAAAG  >  NC_002947/527854‑528058
                                                                                                                                      |                                                                         
aGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCt                                                                       <  2:117511/136‑1 (MQ=17)
aGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCt                                                                       >  1:391247/1‑136 (MQ=32)
aGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCt                                                                       <  2:579762/136‑1 (MQ=32)
   tCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTgg                                                                     >  1:551397/1‑136 (MQ=0)
               tcgGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGt                                                        >  1:460044/1‑136 (MQ=32)
               tcgGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAg                                                         >  2:650262/1‑136 (MQ=0)
                    cTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGaa                                                   >  2:354611/1‑136 (MQ=32)
                         cgGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTg                                              <  1:140452/136‑1 (MQ=32)
                           gAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                            <  2:661182/136‑1 (MQ=32)
                           gAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                            <  2:454076/136‑1 (MQ=32)
                            aaGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                            <  2:30331/136‑1 (MQ=0)
                             tgATGTAACGGGGCTCAAACCTTGCTCCGAAGGTACGGGTATGA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:627790/135‑1 (MQ=255)
                             aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCT‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:358307/136‑1 (MQ=17)
                             aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:163685/136‑1 (MQ=32)
                             aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:546473/136‑1 (MQ=32)
                             aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:416454/136‑1 (MQ=32)
                             aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:511246/136‑1 (MQ=32)
                             aGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACa                                           <  2:544602/136‑1 (MQ=0)
                              gATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGTATGCTGACATg                                         <  2:415240/136‑1 (MQ=17)
                              gATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATg                                         <  2:226471/136‑1 (MQ=32)
                              gATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATg                                         <  2:562375/136‑1 (MQ=32)
                              gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:623273/136‑1 (MQ=0)
                              gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGATGTATCAGAAGTGCGAATGCTGACAt                                          <  2:24214/136‑1 (MQ=0)
                              gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:231097/136‑1 (MQ=0)
                              gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:21523/136‑1 (MQ=0)
                               aTGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACAta                                         <  2:233666/136‑2 (MQ=0)
                                         gCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACaa                              <  2:339759/136‑1 (MQ=32)
                                           tCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATg                            >  1:262908/1‑136 (MQ=32)
                                            cAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGc                           >  2:262715/1‑136 (MQ=32)
                                                      aCCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGaaaaa                 >  1:480716/1‑136 (MQ=32)
                                                              tACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACg         <  2:135122/136‑1 (MQ=32)
                                                                   ttATCA‑TTTTA‑‑CGATTATGCGGTTGTGTAGGTTTCTTTAAGCCTGTTATGGTGTGTTTTGAAGCTTGCTGGTGTTATCAGAAGTGCGAATGCTGACATTTGTAACGACAATGCGTGTGAAAAACTCGCACGCCGaa    <  2:460286/135‑1 (MQ=2)
                                                                   ttATCA‑TCTTA‑‑CTATTATGCGGTTGAGGAGGGTTCTGTATGCCTGTGAAGGTTTGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGGTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa    <  2:133794/135‑1 (MQ=11)
                                                                   ttATCA‑TCTTA‑‑CGATGATGCGGTAGAGGTGCGTTCTGTAAGCCTGTGATGGTGTGTTGAGAAGCTTGCTGTAGGTATCTGATGTGCGAATGCTGACATGTGTAACGACAATGCGTGTGAAAAACTCGCACGCCGaa    <  2:642119/135‑1 (MQ=11)
                                                                   gtttca‑tcTTA‑‑CGATGATGGGGTAGAGGAGGGTTGTGTAAGCCTGTGATGGTGTGTTGATAAGCTTGCTGGAGGTATCAGATGTGCTAATGCTGACATGAGTAACGACAATGCGTGTGAAAAACTCGCACGCCGaa    <  2:360482/133‑1 (MQ=11)
                                                                   gtttca‑taTTA‑‑CGATTTTGCGTTAGAGGAGCGTTCTGTAAGCCTGTGATGGTGAGTTGAGATGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa    <  2:486053/133‑1 (MQ=11)
                                                                   gTATCT‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTTTGAAGGTGTGTTGAGATGCTTGCGGGAGGTATCAGAAGTGCGAATGCTGACATGATTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa    <  2:455972/136‑1 (MQ=11)
                                                                   gTATCA‑TCTTA‑‑CGATGTGGCGGTAGAGGAGCGTTCTGTAAGCCTGTGATGGTGAGTTGAGAAGCTTGCTGGAGTTATCAGAAGTGCTAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa    <  2:404425/136‑1 (MQ=11)
                                                                   gTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa    <  2:496150/136‑1 (MQ=32)
                                                                   gTATCA‑TATTA‑‑CGTTTATGCGGTAGAGGAGCGTTCTTTAAGCCTGTTTAGGTTTGTTGATAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGTGTGAAAACCTCGCACGCCGaa    <  2:130845/136‑1 (MQ=11)
                                                                     atca‑tcTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGAAAg  <  2:435294/136‑1 (MQ=32)
                                                                                 cGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          >  1:647314/1‑87 (MQ=31)
                                                                                 cGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:647314/87‑1 (MQ=31)
                                                                                                                                      |                                                                         
AGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGAAAG  >  NC_002947/527854‑528058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑