Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	543,387	G→A	22.0%	A542T (GCC→ACC)	rpoC →	DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	543,387	0	G	A	22.0%	35.1 / 2.3	18	A542T (GCC→ACC)	rpoC	DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base G (9/5);  new base A (2/2);  total (11/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACATGACCCGGGAAGCCATCAATGCCAAGGGCGAAGGTCGCGTGTTCGCCGACCTGCAGGAAGTCGACCGCGTATTCCGCGCCGGCGAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTGAAAGAGCGTGATGGCTCGGTGGTCAAGAACACCCGCATCGTTGACACCACCGTCGGCCGTGCGCTGCTGTTCCAGGTTGTGCCGGCAG  >  NC_002947/543296‑543519                                                                                            |                                                                                                                                     ctacATGACCCGGGAAGCCATCAATGCCAAGGGCGAAGGTCGCGTGTTCGCCGACCTGCAGGAAGTCGACCGCGTATTCCGCGCCGGCGAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTg                                                                                          >  2:12246/1‑136 (MQ=255) ctacATGACCCGGGAAGCCATCAATGCCAAGGGCGAAGGTCGCGTGTTCGCCGACCTGCAGGAAGTCGACCGCGTATTCCGCGCCGGCGAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTg                                                                                          >  1:648649/1‑136 (MQ=255)            gggAAGCCATCAATGCCAAGGGCGAAGGTCGCGTGTTCGCCGACCTGCAGGAAGTCGACCGCGTATTCCGCGCCGGCGAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTGAAAGAGCGTGa                                                                               >  1:186427/1‑136 (MQ=255)            gggAAGCCATCAATGCCAAGGGCGAAGGTCGCGTGTTCGCCGACCTGCAGGAAGTCGACCGCGTATTCCGCGCCGGCGAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTGAAAGAGCGTGa                                                                               >  1:8930/1‑136 (MQ=255)                           ccAAGGGCGAAGGTCGCGTGTTCGCCGACCTGCAGGAAGTCGACCGCGTATTCCGCGCCGGCGAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTGAAAGAGCGTGATGGCTCGGTGGTCaa                                                                <  2:644408/136‑1 (MQ=255)                                 gCGAAGGTCGCGTGTTCGCCGACCTGCAGGAAGTCGACCGCGTATTCCGCGCCGGCGAAACCGCCCTGCACGCGAAAATCAAGGtt                                                                                                            <  1:495113/86‑1 (MQ=255)                                 gCGAAGGTCGCGTGTTCGCCGACCTGCAGGAAGTCGACCGCGTATTCCGCGCCGGCGAAACCGCCCTGCACGCGAAAATCAAGGtt                                                                                                            <  1:498212/86‑1 (MQ=255)                                 gCGAAGGTCGCGTGTTCGCCGACCTGCAGGAAGTCGACCGCGTATTCCGCGCCGGCGAAACCGCCCTGCACGCGAAAATCAAGGtt                                                                                                            >  2:495113/1‑86 (MQ=255)                                 gCGAAGGTCGCGTGTTCGCCGACCTGCAGGAAGTCGACCGCGTATTCCGCGCCGGCGAAACCGCCCTGCACGCGAAAATCAAGGtt                                                                                                            >  2:498212/1‑86 (MQ=255)                                            gtgtTCGCCGACCTGCAGGAAGTCGACCGCGTATTCCGCGCCGGCGAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTGAAAGAGCGTGATGGCTCGGTGGTCAAGAACACCCGCATCGTTg                                               <  2:8930/136‑1 (MQ=255)                                                  gCCGACCTGCAGGAAGTCGACCGCGTATTCCGCGCCGGCGAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTGAAAGAGCGTGATGGCTCGGTGGTCAAGAACACCCGCATCGTTGCcaccc                                         >  1:466135/1‑135 (MQ=255)                                                  gCCGACCTGCAGGAAGTCGACCGCGTATTCCGCGCCGGCGAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTGAAAGAGCGTGATGGCTCGGTGGTCAAGAACACCCGCATCGTTGAcacca                                         >  1:462761/1‑136 (MQ=255)                                                                aGTCGACCGCGTATTCCGCGCCGGCGAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTGAAAGAGCGTGATGGCTCGGTGGTCAAGAACACCCGCATCGTTGACACCACCGTCGGCCGTgcg                           <  1:199735/136‑1 (MQ=255)                                                                aGTCGACCGCGTATTCCGCGCCGGCGAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTGAAAGAGCGTGATGGCTCGGTGGTCAAGAACACCCGCATCGTTGACACCACCGTCGGCCGTgcg                           <  1:12246/136‑1 (MQ=255)                                                                        gcgTATTCCGCGCCGGCGAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTGAAAGAGCGTGATGGCTCGGTGGTCAAGAACACCCGCATCGTTGACACCACCGTCGGCCGTGCGCTGCTGtt                   >  1:293294/1‑136 (MQ=255)                                                                                     cGGCGAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTGAAAGAGCGTGATGGCTCGGTGGTCAAGAACACCCGCATCGTTGACACCACCGTCGGCCGTGCGCTGCTGTTCCAGGTTGTGCCg      >  2:306162/1‑136 (MQ=255)                                                                                     cGGCGAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTGAAAGAGCGTGATGGCTCGGTGGTCAAGAACACCCGCATCGTTGACACCACCGTCGGCCGTGCGCTGCTGTTCCAGGTTGTGCCg      >  2:411220/1‑136 (MQ=255)                                                                                         gAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTGAAAGAGCGTGATGGCTCGGTGGTCAAGAACACCCGCATCGTTGACACCACCGTCGGCCGTGCGCTGCTGTTCCAGGTTGTGCCGGCAg  <  1:411220/136‑1 (MQ=255)                                                                                            |                                                                                                                                     CTACATGACCCGGGAAGCCATCAATGCCAAGGGCGAAGGTCGCGTGTTCGCCGACCTGCAGGAAGTCGACCGCGTATTCCGCGCCGGCGAAGCCGCCCTGCACGCGAAAATCAAGGTTCGTATCAACGAAACCGTGAAAGAGCGTGATGGCTCGGTGGTCAAGAACACCCGCATCGTTGACACCACCGTCGGCCGTGCGCTGCTGTTCCAGGTTGTGCCGGCAG  >  NC_002947/543296‑543519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑