Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 630,536 G→T 11.7% S387Y (TCT→TAT)  PP_0544 ← ethanolamine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947630,5360GT11.7% 111.6 / 3.0 34S387Y (TCT→TAT) PP_0544ethanolamine transporter
Reads supporting (aligned to +/- strand):  ref base G (15/15);  new base T (2/2);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

CCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCACCCGGGGTGCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATGACCAAGGCCAGTACCGGGGCCTTGCTCTTGTTGGTTTC  >  NC_002947/630401‑630660
                                                                                                                                       |                                                                                                                            
ccGGCGCCCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCACCCGGGGTGCGGTAGGGGCGCTCCCTCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAg                                                                                                                              >  1:9834/1‑136 (MQ=255)
    cGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCACCCGGGGTGCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAg                                                                                                                          <  2:340792/136‑1 (MQ=255)
       ccACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCACCCGGGGTGCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGt                                                                                                                       >  2:260468/1‑136 (MQ=255)
                     gCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCACCCGGTGTGCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAATACAGGGTAGCGCCAAACACTg                                                                                                         >  2:657723/1‑136 (MQ=255)
                     gCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCACCCGGTGTGCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAATACAGGGTAGCGCCAAACACTg                                                                                                         >  2:614862/1‑136 (MQ=255)
                      ccAGCACCAGCGCCAGGCCGGATGTGAAGATGCCACCCGGGGTGCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGc                                                                                                        <  1:187588/136‑1 (MQ=255)
                        aGCACCAGCGCCAGGCCGGATGTGAAGATGCCACCCGGGGTGCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGcca                                                                                                      >  2:443211/1‑136 (MQ=255)
                                   cAGGCCGGATGTGAAGATGCCACCCGGTGTGCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAATACAGGGTAGCGCCAAACACTGCCACCAGGATCAGc                                                                                           <  1:614862/136‑1 (MQ=255)
                                   cAGGCCGGATGTGAAGATGCCACCCGGTGTGCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAATACAGGGTAGCGCCAAACACTGCCACCAGGATCAGc                                                                                           <  1:657723/136‑1 (MQ=255)
                                             gtgAAGATGCCACCCGGGGTGCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCt                                                                                 >  2:389221/1‑136 (MQ=255)
                                             gtgAAGATGCCACCCGGGGTGCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCt                                                                                 >  1:269624/1‑136 (MQ=255)
                                             gtgAAGATGCCACCCGGGGTGCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCt                                                                                 <  1:263621/136‑1 (MQ=255)
                                                       cACCCGGGGTGCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAg                                                                       <  2:240761/136‑1 (MQ=255)
                                                        aCCCGGGGTGCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGc                                                                      >  1:59055/1‑136 (MQ=255)
                                                                 gCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCa                                                             <  1:443211/136‑1 (MQ=255)
                                                                      aGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAAccc                                                        <  2:402369/136‑1 (MQ=255)
                                                                               ccATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGcc                                               >  1:530092/1‑136 (MQ=255)
                                                                                 aTCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCgg                                             <  1:260468/136‑1 (MQ=255)
                                                                                  tCTTTGGCCGGCGGATGTGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACACGGCAAACCCGATGATGCCggg                                            >  2:270614/1‑136 (MQ=255)
                                                                                  tCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCggg                                            >  2:507044/1‑136 (MQ=255)
                                                                                      tGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCATGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATg                                        <  1:111954/136‑1 (MQ=255)
                                                                                           ggcggATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATGACCaa                                   <  2:317050/136‑1 (MQ=255)
                                                                                           ggcggATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATGACCaa                                   <  2:484685/136‑1 (MQ=255)
                                                                                            gcggATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATGACCAAg                                  <  1:89109/136‑1 (MQ=255)
                                                                                            gcggATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATGACCAAg                                  <  1:37587/136‑1 (MQ=255)
                                                                                                         gTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATGACCAAGGCCAGTACCgggg                     <  1:597613/136‑1 (MQ=255)
                                                                                                                gcgcAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATGACCAAGGCCAGTACCGGGGCCTTGcg              >  2:466524/1‑135 (MQ=255)
                                                                                                                     gCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATGACCAAGGCCAGTACCGGGGCCTTGCTCttgt         >  2:376038/1‑136 (MQ=255)
                                                                                                                     gCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATGACCAAGGCCAGTACCGGGGCCTTGCTCttgt         >  1:290367/1‑136 (MQ=255)
                                                                                                                     gCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATGACCAAGGCCAGTACCGGGGCCTTGCTCttgt         >  1:280678/1‑136 (MQ=255)
                                                                                                                     gCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCAGGGGATGACCAAGGCCAGTACCGGGGCCTTGCACttgt         >  1:72365/1‑136 (MQ=255)
                                                                                                                       catcatCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATg                                        >  1:521064/1‑103 (MQ=255)
                                                                                                                       catcatCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATg                                        <  2:521064/103‑1 (MQ=255)
                                                                                                                         tcatcaGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATGACCAAGGCCAGTACCGGGGCCTTGCTCTTGTTggg     >  1:225742/1‑135 (MQ=255)
                                                                                                                            tcaGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATGACCAAGGCCAGTACCGGGGCCTTGCTCTTGTTGGTTTc  <  2:230074/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                            
CCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCACCCGGGGTGCGGTAGGGGCGCTCCATCTTTGGCCGGCGGATGCGCAGGGTGATGTGCGCAGCCATCATCAGCACGTAAGACAGGGTAGCGCCAAACACTGCCACCAGGATCAGCAAGTCACCCTGGCCCGTCAGCGACAGGGCAAACCCGATGATGCCGGGGATGACCAAGGCCAGTACCGGGGCCTTGCTCTTGTTGGTTTC  >  NC_002947/630401‑630660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑