| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,047,840 | C→T | 100% | S233L (TCG→TTG) | colS → | integral membrane sensor signal transduction histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,047,840 | 0 | C | T | 100.0% | 21.3 / NA | 8 | S233L (TCG→TTG) | colS | integral membrane sensor signal transduction histidine kinase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (6/2); total (6/2) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACGAAGTCGGCCAGCTTGCAGTGGCTTTCGACGATACCCTGGGTCGCCTGCGTGATGCGCTGACCCGCGAGCGGCTGTTCACCAGCGACGTCAGCCACGAGCTGCGCACGCCGTTGATGGTGTTGGCTACCTCGTGTGAGCTTCTGATGGAAAACCCTGGCCTCGATGCCCGCGCGCGTAGCCAGGTGGAGCGCGTGGCGCGGGCCACCGAAGAGATGCGCGAGCTGGTCAAGACCTTCCTCATGTTGGCCCGCGCGCAG > NC_002947/1047708‑1047967 | aCGAAGTCGGCCAGCTTGCAGTGGCTTTCGACGATACCCTGGGTCGCCTGCGTGATGCGCTGACCCGCGAGCGGCTGTTCACCAGCGACGTCAGCCACGAGCTGCGCACGCCGTTGATGGTGTTGGCTACCTtgtg > 1:172264/1‑136 (MQ=255)aCGAAGTCGGCCAGCTTGCAGTGGCTTTCGACGATACCCTGGGTCGCCTGCGTGATGCGCTGACCCGCGAGCGGCTGTTCACCAGCGACGTCAGCCACGAGCTGCGCACGCCGTTGATGGTGTTGGATACCTTgtt > 1:172261/1‑135 (MQ=255) ttCGACGATACCCTGGGTCGCCTGCGTGATGCGCTGACCCGCGAGCGGCTGTTCACCAGCGACGTCAGCCACGAGCTGCGCACGCCGTTGATGGTGTTGGCTACCTTGTGTGAGCTTCTGATGGAAAACCCTGGcc > 2:402527/1‑136 (MQ=255) gCGTGATGCGCTGACCCGCGAGCGGCTGTTCACCAGCGACGTCAGCCACGAGCTGCGCACGCCGTTGATGGTGTTGGCTACCTTGTGTGAGCTTCTGATGGAAAACCCTGGCCTCGATGCCCGCGCGCGTAGCCAg > 1:36296/1‑136 (MQ=255) gcgAGCGGCTGTTCACCAGCGACGTCAGCCACGAGCTGCGCACGCCGTTGATGGTGTTGGCTACCTTGTGTGAGCTTCTGATGGAAAACCCTGGCCTCGATGCCCGCGCGCGTAGCCAGGTGGAGCGCGTGGcgcg < 1:318089/136‑1 (MQ=255) cGACGTCAGCCACGAGCTGCGCACGCCGTTGATGGTGTTGGCTACCTTGTGTGAGCTTCTGATGGAAAACCCTGGCCTCGATGCCCGCGCGCGTAGCCAGGTGGAGCGCGTGGCGCGGGCCACCGAAGAGATgcgc > 1:484642/1‑136 (MQ=255) gACGTCAGCCACGAGCTGCGCACGCCGTTGATGGTGTTGGCTACCTTGTGTGAGCTTCTGATGGAAAACCCTGGCCTCGATGCCCGCGCGCGTAGCCAGGTGGAGCGCGTGGCGCGGGCCACCGAAGAGATGcgcg < 2:36296/136‑1 (MQ=255) aCGCCGTTGATGGTGTTGGCTACCTTGTGTGAGCTTCTGATGGAAAACCCTGGCCTCGATGCCCGCGCGCGTAGCCAGGTGGAGCGCGTGGCGCGGGCCACCGAAGAGATGCGCGAGCTGGTCAAGACCTTCCTCa > 2:4507/1‑136 (MQ=255) ggCTACCTTGTGTGAGCTTCTGATGGAAAACCCTGGCCTCGATGCCCGCGCGCGTAGCCAGGTGGAGCGCGTGGCGCGGGCCACCGAAGAGATGCGCGAGCTGGTCAAGACCTTCCTCATGTTGGCCCGCGCGCAg > 2:44655/1‑136 (MQ=255) | ACGAAGTCGGCCAGCTTGCAGTGGCTTTCGACGATACCCTGGGTCGCCTGCGTGATGCGCTGACCCGCGAGCGGCTGTTCACCAGCGACGTCAGCCACGAGCTGCGCACGCCGTTGATGGTGTTGGCTACCTCGTGTGAGCTTCTGATGGAAAACCCTGGCCTCGATGCCCGCGCGCGTAGCCAGGTGGAGCGCGTGGCGCGGGCCACCGAAGAGATGCGCGAGCTGGTCAAGACCTTCCTCATGTTGGCCCGCGCGCAG > NC_002947/1047708‑1047967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |