Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,094,030 T→C 7.9% L107L (TTG→CTG)  PP_0943 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,094,0300TC 7.9% 147.7 / 2.7 50L107L (TTG→CTG) PP_0943hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/28);  new base C (2/2);  total (20/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCGATCGCATGCTCCCCTGGCATTGGCGTTGTCTGTGCCTGGACCAGGCGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTATCGAGCGTGACAGCATGGGTGAGCTGCAGGTGCC  >  NC_002947/1093909‑1094146
                                                                                                                         |                                                                                                                    
cTGCGATCGCATGCTCCCCTGGCATTGGCGTTGTCTGTGCCTGGACCAGGCGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAAt                                                                                                        >  1:604105/1‑136 (MQ=255)
  gCGATCGCATGCTCCCCTGGCATTGGCGTTGTCTGTGCCTGGACCAGGCGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTg                                                                                                      >  2:624011/1‑136 (MQ=255)
      tCGCATGCTCCCCTGGCATTGGCGTTGTCTGTGCCTGGACCAGGCGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGt                                                                                                  <  2:536896/136‑1 (MQ=255)
       cGCATGCTCCCCTGGCATTGGCGTTGTCTGTGCCTGGACCAGGCGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTc                                                                                                 <  1:201572/136‑1 (MQ=255)
                         tGGCGTTGTCTGTGCCTGGACCAGGCGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTtctc                                                                               <  1:53430/136‑1 (MQ=255)
                             gTTGTCTGTGCCTGGACCAGGCGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGACCCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGa                                                                           >  1:582080/1‑136 (MQ=255)
                             gTTGTCTGTGCCTGGACCAGGCGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCgc                                                                           >  1:541289/1‑135 (MQ=255)
                              ttGTCTGTGCCTGGACCAGGCGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGAt                                                                          <  2:52107/136‑1 (MQ=255)
                                   tgtgCCTGGACCAGGCGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACt                                                                     >  1:40818/1‑136 (MQ=255)
                                   tgtgCCTGGACCAGGCGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACt                                                                     >  2:329824/1‑136 (MQ=255)
                                              cAGGCGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAg                                                             <  1:400411/133‑1 (MQ=255)
                                              cAGGCGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAg                                                             >  2:400411/1‑133 (MQ=255)
                                              cAGGCGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGAt                                                          >  1:633027/1‑136 (MQ=255)
                                                  cGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTtga                                                      >  2:371920/1‑136 (MQ=255)
                                                   gTGGCGCCGTCTGCTGGACCTGCGCAACCTCGACAGCCAGGATCAGATCGAACGCTGGGAAACCTACGCCTTGCAGTTGTCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTtgat                                                     <  1:415555/136‑1 (MQ=255)
                                                   gTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATTAACTGATGCAAGGATTtgat                                                     <  1:171379/136‑1 (MQ=255)
                                                   gTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTtgat                                                     <  1:638515/136‑1 (MQ=255)
                                                   gTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTtgat                                                     <  2:146111/136‑1 (MQ=255)
                                                   gTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTtgat                                                     <  2:527825/136‑1 (MQ=255)
                                                    gggCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCAGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTtgatg                                                    <  1:118361/135‑1 (MQ=255)
                                                       cgcCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCAGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGGTTTCTCCCGATGAACTGATGCAAGGATTtgatgatg                                                 <  2:118609/136‑1 (MQ=255)
                                                         cccTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCGTTTCAGTTGGCCAATTGCCTTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAg                                               <  1:487799/136‑1 (MQ=255)
                                                         cccTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAg                                               <  1:624011/136‑1 (MQ=255)
                                                         cccTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCACCCGATGAACTGATGCAAGGATTTGATGATGAg                                               >  1:192662/1‑136 (MQ=255)
                                                            tctGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTATATTTCTCCCGATGAACTGATGCTAGGATTttttgatttttga                                            <  1:575182/136‑7 (MQ=255)
                                                              tgctgGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATa                                          >  2:575182/1‑136 (MQ=255)
                                                              tgctgGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATa                                          >  2:328953/1‑136 (MQ=255)
                                                                 tgGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATAccc                                       <  2:40690/136‑1 (MQ=255)
                                                                 tgGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATAccc                                       <  2:414965/136‑1 (MQ=255)
                                                                           cAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAg                                               >  2:572979/1‑118 (MQ=255)
                                                                           cAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAg                                               <  1:572979/118‑1 (MQ=255)
                                                                           cAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTATCGAGCg                             <  2:120567/136‑1 (MQ=255)
                                                                           cAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTATCGAGCg                             >  2:362313/1‑136 (MQ=255)
                                                                           cAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTATCGAGCg                             <  1:367393/136‑1 (MQ=255)
                                                                           cAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTATCGAGCg                             >  2:62988/1‑136 (MQ=255)
                                                                           cAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCCTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATTAACTGATGCAAGGATTtgat                                                     >  1:617604/1‑112 (MQ=255)
                                                                           cAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCCTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTtgat                                                     <  2:617610/112‑1 (MQ=255)
                                                                           cAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCCTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTtgat                                                     >  1:617610/1‑112 (MQ=255)
                                                                           cAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCCTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTtgat                                                     <  2:617604/112‑1 (MQ=255)
                                                                                    ccGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTATCGAGCGTGACAGCAt                    <  1:329824/136‑1 (MQ=255)
                                                                                           gAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTTTCGAGCGTGACAGCATGGGTGAg             <  1:553807/136‑1 (MQ=255)
                                                                                           gAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTATCGAGCGTGACAGCATGGGTGAg             >  2:354412/1‑136 (MQ=255)
                                                                                                gAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTATCGAGCGTGACAGCATGGGTGAGCTGCa        <  1:285838/136‑1 (MQ=255)
                                                                                                caaCCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTATCGAGCGTGACAGCATGGGTGAGCTGCa        <  1:134484/135‑1 (MQ=255)
                                                                                                  aCCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTATCGAGCGTGACAGCATGGGTGAGCTGCAgg      <  2:40818/136‑1 (MQ=255)
                                                                                                  aCCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTATCGAGCGTGACAGCATGGGTGAGCTGCAgg      <  2:143861/136‑1 (MQ=255)
                                                                                                  aCCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTATCGAGCGTGACAGCATGGGTGAGCTGCAgg      <  1:377055/136‑1 (MQ=255)
                                                                                                  aCCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTATCGAGCGTGACAGCATGGGTGAGCTGCAgg      <  2:665892/136‑1 (MQ=255)
                                                                                                     aaCTCTGGCAACACTACGACTTTCAGATGGCCAAATGACGTCTGCTGCAGTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTTATAAACGTATCGAGCGTGACAGCATGGGTGAGATGCAGTTGc   >  1:153003/1‑136 (MQ=255)
                                                                                                      aCGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTATCGAGCGTGACAGCATGGGTGAGCTGCAGGTGcc  >  2:568089/1‑136 (MQ=255)
                                                                                                                         |                                                                                                                    
CTGCGATCGCATGCTCCCCTGGCATTGGCGTTGTCTGTGCCTGGACCAGGCGTGGCGCCCTCTGCTGGACCTGCGCAACCTCGACCGCCAGGAACAGAACCAACGCTGGCAACCCTACGCCTTGCAGTTGGCCAATTGCCGTCTGCTGCCTTCGATTTCTCCCGATGAACTGATGCAAGGATTTGATGATGAGTGATACCCGTATCGAGCGTGACAGCATGGGTGAGCTGCAGGTGCC  >  NC_002947/1093909‑1094146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑