Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,144,107 C→T 13.8% A165T (GCC→ACC)  arcB ← ornithine carbamoyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,144,1070CT13.8% 74.5 / 4.2 29A165T (GCC→ACC) arcBornithine carbamoyltransferase
Reads supporting (aligned to +/- strand):  ref base C (15/10);  new base T (2/2);  total (17/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.09e-01

CGTACTGCTTGCAGCGCTCGACCAGGTCGTCATGTGGCCACAGTGCCTTGGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAGCACGTCGGCGATCATCTGGGTCGGGTGGTATTCGTCGGTCAGGCCGTTGAACACCGGTACGCCGGCGAAC  >  NC_002947/1143984‑1144240
                                                                                                                           |                                                                                                                                     
cGTACTGCTTGCAGCGCTCGACCAGGTCGTCATGTGGCCACAGTGCCTTGGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTa                                                                                                                           <  1:368954/136‑1 (MQ=255)
             gcgcTCGACCAGGTCGTCATGTGGCCACAGTGCCTTGGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATg                                                                                                              >  2:362111/1‑136 (MQ=255)
             gcgcTCGACCAGGTCGTCATGTGGCCACAGTGCCTTGGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATg                                                                                                              >  1:541039/1‑136 (MQ=255)
               gcTCGACCAGGTCGTCATGTGGCCACAGTGCCTTGGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTc                                                                                                            >  2:356384/1‑136 (MQ=255)
                  cgcccAGGTCGTCATGTGGCCACAGTGCCTTGGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGCTGTCGTg                                                                                                         >  2:57046/4‑136 (MQ=255)
                  cGACCAGGTCGTCATGTGGCCACAGTGCCTTTGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTg                                                                                                         >  2:630324/1‑136 (MQ=255)
                  cGACCAGGTCGTCATGTGGCCACAGTGCCTTGGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGTATCGCCCAGGTAGGCGTAGCTGATGTCGTg                                                                                                         >  2:660123/1‑136 (MQ=255)
                  cGACCAGGTCGTCATGTGGCCACAGTGCCTTGGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGTATCGCCCAGGTAGGCGTAGCTGATGTCGTg                                                                                                         >  2:657158/1‑136 (MQ=255)
                             tcATGTGGCCACAGTGCCTTGGGCGCGGCGATGCGCAAATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGACTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGt                                                                                              >  2:607815/1‑136 (MQ=255)
                              cATGTGGCCACAGTGCCTTGGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGTATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTc                                                                                             <  1:660123/136‑1 (MQ=255)
                              cATGTGGCCACAGTGCCTTGGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGTATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTc                                                                                             <  1:657158/136‑1 (MQ=255)
                              cATGTGGCCACAGTGCCTTGGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTc                                                                                             <  2:310802/136‑1 (MQ=255)
                                tgtgGCCACAGTGCCTTGGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGc                                                                                           >  1:536835/1‑136 (MQ=255)
                                tgtgGCCACAGTGACTTGGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGc                                                                                           >  1:508674/1‑136 (MQ=255)
                                   ggCCACAGTGCCTTGGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCtgt                                                                                        <  1:630324/136‑1 (MQ=255)
                                                   gcgcGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAg                                                                        >  1:73428/1‑136 (MQ=255)
                                                       ggcgATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAGCACg                                                                    <  1:391254/136‑1 (MQ=255)
                                                          gATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAGCACGTCg                                                                 >  1:284181/1‑136 (MQ=255)
                                                                 acaTCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAGCACGTCGGCGatca                                                          <  2:73428/136‑1 (MQ=255)
                                                                    tCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAGCACGTCGGCGATCATCt                                                       <  1:92768/136‑1 (MQ=255)
                                                                    tCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAGCACGTCGGCGATCATCt                                                       <  1:595246/136‑1 (MQ=255)
                                                                         gCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAGCACGTCGGCGATCATCTGGGTc                                                  <  1:300767/136‑1 (MQ=255)
                                                                           ccAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAGCACGTCGGCGATCATCTGGGTCgg                                                >  2:498528/1‑136 (MQ=255)
                                                                                ttGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAGCACGTCGGCGATCATCTGGGTCGggtggt                                           >  1:63794/1‑136 (MQ=255)
                                                                                            agcagcagcGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAGCACGTCGGCGATCATCTGGGTCGGGTGGTATTCGTCGGTCa                               >  1:297697/1‑136 (MQ=255)
                                                                                                                 ttgttgCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAGCACGTCGGCGATCATCTGGGTCGGGTGGTATTCGTCGGTCAGGCCGTTGAACACCGGTACGc          <  1:498048/136‑1 (MQ=255)
                                                                                                                 ttgttgCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAGCACGTCGGCGATCATCTGGGTCGGGTGGTATTCGTCGGTCAGGCCGTTGAACACCGGTACGc          <  1:362111/136‑1 (MQ=255)
                                                                                                                       cGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAGCACGTCGGCGATCATCTGGGTCGGGTGGTATTCGTCGGTCAGGCCGTTGAACACCGGTACGCCGGCGa    >  2:159807/1‑136 (MQ=255)
                                                                                                                         ggCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAGCACGTCGGCGATCATCTGGGTCGGGTGGTATTCGTCGGTCAGGCCGTTGAACACCGGTACGCCGGCGAAc  >  2:346657/1‑136 (MQ=255)
                                                                                                                           |                                                                                                                                     
CGTACTGCTTGCAGCGCTCGACCAGGTCGTCATGTGGCCACAGTGCCTTGGGCGCGGCGATGCGCACATCCATGCCCAGCTTGGCGCCGATCAGCAGCAGCGAGTTGCCCATGTTGTTGCGGGCATCGCCCAGGTAGGCGTAGCTGATGTCGTGCAGCGGCTTGTCGCTGTGCTCACGCATGGTCAGCACGTCGGCGATCATCTGGGTCGGGTGGTATTCGTCGGTCAGGCCGTTGAACACCGGTACGCCGGCGAAC  >  NC_002947/1143984‑1144240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑