| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,310,148 | C→A | 14.8% | M68I (ATG→ATT) | livM ← | branched‑chain amino acid ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,310,148 | 0 | C | A | 14.8% | 78.5 / 3.4 | 27 | M68I (ATG→ATT) | livM | branched‑chain amino acid ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base C (15/8); new base A (2/2); total (17/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01 | |||||||||||
ACCACGAGAGCCGAAGAACGGCCATACCAGTGCCACCACGATCAACCCGATGATGACATAGCGCTGGGTCTTGGGCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCGATGATGCTGATGGTGAACGAGCCCTGACTGTGCACGACCAGGCTGATGCCGTCGATGCTCAGTTTCAG > NC_002947/1310016‑1310279 | accacGAGAGCCGAAGAACGGCCATACCAGTGCCACCACGATCAACCCGATGATGACATAGCGCTCGGTCTTGGGCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATc > 2:539488/1‑136 (MQ=255) ccGAAGAACGGCCATACCAGTGCCACCACGATCAACCCGATGATGACATAGCGCTGGGTCTTGGGCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTcc < 2:408153/136‑1 (MQ=255) aagaaCGGCCATACCAGTGCCACCACGATCAACCCGATGATGACATAGCGCTGGGTCTTGGGCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACt > 2:11808/1‑136 (MQ=255) aTACCAGTGCCACCACGATCAACCCGATGATGACATAGCGCTGGGTCTTGGGCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGaa > 1:450777/1‑136 (MQ=255) caccacGATCAACCCGATGATGACATAGCGCTGGGTCTTGGGCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCAATCACCGAACTCCACTGCTTGTCGAACAGCACgcgc > 1:142170/1‑136 (MQ=255) ccacGATCAACCCGATGATGACATAGCGCTGGGTCTTGGGCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAg > 2:442608/1‑136 (MQ=255) gatgatGACATAGCGCTGGGTCTTGGGCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACg > 2:361855/1‑136 (MQ=255) tAGCGCTGGGTCTTGGGCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACgg > 2:621913/1‑136 (MQ=255) gcTGGGTCTTGGGCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCgat > 1:459481/1‑136 (MQ=255) gcTGGGTCTTGGGCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCgat > 1:667202/1‑136 (MQ=255) cTTGGGCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCGATGATGCTg > 2:338414/1‑136 (MQ=255) cTTGGGCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCGATGATGCTg > 1:445486/1‑136 (MQ=255) gCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCGATGATGCTGATGGt < 1:11808/136‑1 (MQ=255) gCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCGATGATGCTGATGGt < 1:544400/136‑1 (MQ=255) aGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCGATGATGCTGATGGTGa < 2:445486/136‑1 (MQ=255) aGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCGATGATGCTGATGGTGa > 2:598246/1‑136 (MQ=255) gAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCGATGATGCTGATGGTGAACGAg > 1:656782/1‑136 (MQ=255) gAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCGATGATGCTGATGGTGAACGAg > 1:54998/1‑136 (MQ=255) gAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCGATGATGCTGATGGTGAACGAg > 1:227278/1‑136 (MQ=255) gAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCGATGATGCTGATGGTGAACGAg > 1:224214/1‑136 (MQ=255) gAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCAATCACCGAACTcc < 2:377795/65‑1 (MQ=255) gAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCAATCACCGAACTcc > 1:377795/1‑65 (MQ=255) gTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCGATGATGCTGATGGTGAACGAGccc < 1:442608/136‑1 (MQ=255) cTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCAATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCGATGATGCTGATGGTGAACGAGCCCTGACtgt < 2:142170/136‑1 (MQ=255) ggATCAGCTTGCGATCCGAGCGGCGGCCCACCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGCAACATCAGCACGGAACACACGGCGATGATGCTGATGGTGAACGAGCCCTGACTGTGCACGACCAGGCt < 2:548488/136‑1 (MQ=255) aTCAGCTTGCGATCCGATGGGCGGCCCTTCACCGAACTCCACTGATTGTCGAACAGCACGCGCAGGAAAATCAGCCCGGAACACACGGCGATGATGCTGATGGTGAACGAGCCCTGACTGTGCACGACCAGGCTGa < 2:132773/136‑1 (MQ=255) ggcCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCGATGATGCTGATGGTGAACGAGCCCTGACTGTGCACGACCAGGCTGATGCCGTCGATGCTCAGTTTCAg < 1:598246/136‑1 (MQ=255) | ACCACGAGAGCCGAAGAACGGCCATACCAGTGCCACCACGATCAACCCGATGATGACATAGCGCTGGGTCTTGGGCAGGGTGAGGTAGTTGCTGACTGCCGGGGGGATCAGCTTGCGATCCGATCGGCGGCCCATCACCGAACTCCACTGCTTGTCGAACAGCACGCGCAGGAACATCAGCACGGAACACACGGCGATGATGCTGATGGTGAACGAGCCCTGACTGTGCACGACCAGGCTGATGCCGTCGATGCTCAGTTTCAG > NC_002947/1310016‑1310279 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |