| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 210,239 | G→T | 18.2% | D5249Y (GAC→TAC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 210,239 | 0 | G | T | 18.2% | 50.3 / 3.8 | 22 | D5249Y (GAC→TAC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (12/6); new base T (2/2); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01 | |||||||||||
CGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTC > NC_002947/210114‑210370 | cgcAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACcga > 2:668950/1‑136 (MQ=37) tttACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCaa < 2:418877/136‑1 (MQ=37) aaCGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGa > 1:471219/1‑136 (MQ=37) cTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGAc > 2:594617/1‑136 (MQ=40) aGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTc > 2:365770/1‑136 (MQ=255) cACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAggcggc < 1:668950/136‑1 (MQ=255) cTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCgag > 2:544130/1‑136 (MQ=255) cggTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCAc > 2:394096/1‑136 (MQ=255) gACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTacc > 2:104217/1‑135 (MQ=255) gCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCa > 2:171486/1‑136 (MQ=255) gCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGT‑‑‑‑‑‑‑‑GGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTg > 2:666921/1‑136 (MQ=11) gTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCt < 1:544130/136‑1 (MQ=255) gACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGAcc > 2:151862/1‑136 (MQ=255) ttCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCTACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCTGCGAGATCACCTACACCATCACCCTGACcaaca > 1:624381/1‑136 (MQ=255) ttCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCTACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCTGCGAGATCACCTACACCATCACCCTGACcaaca > 1:314305/1‑136 (MQ=255) tGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCt < 2:77050/136‑1 (MQ=255) caacTGGGCGGTGCGGCTACCGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGc > 2:371144/4‑136 (MQ=11) ggTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATcaaca > 2:135793/1‑136 (MQ=255) cGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGc < 1:216431/136‑1 (MQ=37) cGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGc < 1:151862/136‑1 (MQ=37) gACCTACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCTGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTc < 2:624381/136‑1 (MQ=255) gACCTACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCTGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTc < 2:314305/136‑1 (MQ=255) | CGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTC > NC_002947/210114‑210370 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |