Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,543,926 C→A 14.8% L545L (CTG→CTT)  PP_1350 ← two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,543,9260CA14.8% 79.1 / 3.5 27L545L (CTG→CTT) PP_1350two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base C (11/12);  new base A (2/2);  total (13/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

GGGTTTAGCCGGGTGCGCAGTGTGATGCGTCCGGGCTCGCTGGGTTGCGGGCGTTGGTGGATGGCCTGGGCTGCGTTCTTCAGCAAGTTGAGCAGTACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCAGTGCCGGCAGCTCGCATGGCACCAGCTGGCGGTT  >  NC_002947/1543792‑1544060
                                                                                                                                      |                                                                                                                                      
gggTTTAGCCGGGTGCGCAGTGGGATGCGTCCGGGCTCGCTGGGTTGCGGGCGTTGGTGGATGGCCTGGGCTGCGTTCTTCAGCAAGTTGAGCAGTACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCCa                                                                                                                                       <  1:563576/136‑1 (MQ=255)
                    tgtgATGCGTCCGGGCTCGCTGGGTTGCGGGCGTTGGTGGATGGCCTGGGCTGCGTTCTTCAGCAAGTTGAGCAGTACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGgcgc                                                                                                                   <  1:52719/136‑1 (MQ=255)
                        aTGCGTCCGGGCTCGCTGGGTTGCGGGCGTTGGTGGATGGCCTGGGCTGCGTTCTTCAGCAAGTTGAGCAGTACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGa                                                                                                               <  2:481106/136‑1 (MQ=255)
                                 ggCTCGCTGGGTTGCGGGCGTTGGTGGATGGCCTGGGCTGCGTTCTTCAGCAAGTTGAGCAGTACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGcc                                                                                                      >  1:44892/1‑136 (MQ=255)
                                 ggCTCGCTGGGTTGCGGGCGTTGGTGGATGGCCTGGGCTGCGTTCTTCAGCAAGTTGAGCAGTACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCAAGGTAGGGGTCGAACTGGCGCACGATGGCTTGcc                                                                                                      >  2:11463/1‑136 (MQ=255)
                                 ggCTCGCTGGGTTGCGGGCGTTGGTGGATGGCCTGGGCTGCCATCTTCAGCAAGTTGAGCAGTACTAGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCAAGGTAGGGGTCGAACTGGCGCACCATCGCATGcc                                                                                                      >  2:481607/1‑136 (MQ=255)
                                  gCTCGCTTGGTTGCGGGCGTTGGTGGATGGCCTGGGCTGCGTTCTTCAGCAAGTTGAGCAGTACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGccc                                                                                                     <  1:503293/136‑1 (MQ=255)
                                                                            tcttcAGCAAGTTGAGCAGTACTTGTTCCAGCTTGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGc                                                           >  1:62965/1‑136 (MQ=255)
                                                                            tcttcAGCAAGTTGAGCAGTACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGc                                                           >  1:368801/1‑136 (MQ=255)
                                                                                    aaGTTGAGCAGTACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCTAAGTCATTGCCGGCAATTTCaa                                                   <  2:51885/136‑1 (MQ=255)
                                                                                         gAGCAGTACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGcc                                              <  1:223247/136‑1 (MQ=255)
                                                                                         gAGCAGTACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGcc                                              <  1:590699/136‑1 (MQ=255)
                                                                                         gAGCAGTACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGGCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGcc                                              <  1:224063/136‑1 (MQ=255)
                                                                                             aGTACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTgat                                          <  2:473032/136‑1 (MQ=255)
                                                                                               tACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTgatcg                                        <  1:154670/136‑1 (MQ=255)
                                                                                               tACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCAAGGTAGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTgatcg                                        <  1:481607/136‑1 (MQ=255)
                                                                                               tACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCAAGGTAGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTgatcg                                        <  1:11463/136‑1 (MQ=255)
                                                                                                       ccAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCAGTGc                                >  1:465628/1‑136 (MQ=255)
                                                                                                       ccAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCAGTGc                                <  2:628201/136‑1 (MQ=255)
                                                                                                       ccAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCCATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCAGTGc                                <  1:437952/136‑1 (MQ=255)
                                                                                                            tCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCATTCCCCGCa                           >  2:80419/1‑136 (MQ=255)
                                                                                                            tCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCAGTGCCGGCa                           >  1:252646/1‑136 (MQ=255)
                                                                                                            tCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCAGTGCCGGCa                           >  2:50971/1‑136 (MQ=255)
                                                                                                            tCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCAGTGCCGGCa                           >  2:620458/1‑136 (MQ=255)
                                                                                                            tCGTAGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCAGTGCCGGCa                           >  2:389310/1‑136 (MQ=255)
                                                                                                              gTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCAGTGCCGGCAGc                         >  2:452214/1‑136 (MQ=255)
                                                                                                                              aCCGGCCACAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCAGTGCCGGCAGCTCGCATGGCACCAGCt         >  2:629470/1‑136 (MQ=255)
                                                                                                                                   ccccAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCAGTGCCGGCAGCTCGCATGGCACCAGCTGGCgg    >  1:402290/1‑136 (MQ=255)
                                                                                                                                   ccccAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCAGTGCCGGCAGCTCGCATGGCACCAGCTGGCgg    >  1:39376/1‑136 (MQ=255)
                                                                                                                                     ccAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCAGTGCCGGCAGCTCGCATGGCACCAGCTGGCGGtt  >  2:157353/1‑136 (MQ=255)
                                                                                                                                     ccAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCAGTGCCGGCAGCTCGCATGGAACCAGCTGGCGGtt  >  1:294312/1‑136 (MQ=255)
                                                                                                                                      |                                                                                                                                      
GGGTTTAGCCGGGTGCGCAGTGTGATGCGTCCGGGCTCGCTGGGTTGCGGGCGTTGGTGGATGGCCTGGGCTGCGTTCTTCAGCAAGTTGAGCAGTACTTGTTCCAGCTCGTTGGCGGTACATGGCACCGGCCCCAGGTCGGGGTCGAACTGGCGCACGATGGCTTGCCCCTTGAAGTCGAAGCCGATGGCCAGATCGAAGTCATTGCCGGCAATTTCAAGCGCCTGATCGATCAGTGCCGGCAGCTCGCATGGCACCAGCTGGCGGTT  >  NC_002947/1543792‑1544060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑