| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,585,535 | G→A | 20.0% | P285S (CCG→TCG) | ttgB ← | efflux pump membrane protein TtgB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,585,535 | 0 | G | A | 20.0% | 38.6 / 4.9 | 20 | P285S (CCG→TCG) | ttgB | efflux pump membrane protein TtgB |
| Reads supporting (aligned to +/- strand): ref base G (7/9); new base A (2/2); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01 | |||||||||||
CCCGGCGGGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGATACTCTCGAACTGCTCG > NC_002947/1585429‑1585659 | cccGGCGGGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGt < 2:159943/136‑1 (MQ=255) ggggAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGTAGttt < 2:401938/135‑1 (MQ=255) cggGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGTAGttt > 1:467979/1‑136 (MQ=255) agaaCGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCAcc < 1:416019/136‑1 (MQ=255) tcGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACg < 2:465437/96‑1 (MQ=255) tcGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACg > 1:465437/1‑96 (MQ=255) gcAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGTGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAg < 1:199403/136‑1 (MQ=255) gcAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAg < 2:262503/136‑1 (MQ=255) cTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCAcc > 1:6164/1‑136 (MQ=255) tGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGACTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCtg > 2:143117/1‑136 (MQ=255) tGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGACTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCtg > 2:146278/1‑136 (MQ=255) gCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCtgtg < 1:287471/136‑1 (MQ=255) tCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGt > 1:290206/1‑136 (MQ=255) tCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGCCGCTGACGGCGTAGATTTCACCCCCCAGGCCTACTTGGGCGACAACGCCCAGGCGCACCTGTGAACCGt > 1:415954/1‑136 (MQ=255) cgcCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAg < 1:160791/106‑1 (MQ=255) cgcCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAg > 2:160791/1‑106 (MQ=255) cgcCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGACTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCtt < 1:146278/136‑1 (MQ=255) cgcCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGACTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCtt < 1:143117/136‑1 (MQ=255) gTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGATAct > 2:576452/1‑136 (MQ=255) gCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGATACTCTCGAACTGCTcg < 1:211571/136‑1 (MQ=255) | CCCGGCGGGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGATACTCTCGAACTGCTCG > NC_002947/1585429‑1585659 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |