Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,120,159 G→A 10.5% V229V (GTG→GTA)  PP_1883 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,120,1590GA10.5% 110.6 / 2.8 38V229V (GTG→GTA) PP_1883hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (16/18);  new base A (2/2);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCAGGAGTCTGATCCTGGCCGACCACTGCTGTGGCACCTGGATATCGGCCAGGTCTTCATGTATCTCGGCGGCTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGGCGCGCTGGATCCTGATGACGAAACCTATCGCGCCGGCCTTCTG  >  NC_002947/2120025‑2120291
                                                                                                                                      |                                                                                                                                    
gTGCAGGAGTCTGATCCTGGCCGACCACTGCTGTGGCACCTGGATATCGGCCAGGTCTTCATGTATCTCGGCGGCTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCAACAGCAGCCGGTCGCCAGAGc                                                                                                                                     >  2:20222/1‑136 (MQ=255)
      gAGTCTGATCCTGGCCGACCACTGCTGTGGCACCTGGATATCGGCCAGGTCTTCATGTATCTCGGCGGCTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTACAGCGCt                                                                                                                               >  1:58598/1‑136 (MQ=255)
      gAGTCTGATCCTGGCCGACCACTGCTGTGGCACCTGGATATCGGCCAGGTCTTCATGTATCTCGGCGGCTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTACAGCGCt                                                                                                                               >  1:55337/1‑136 (MQ=255)
        gTCTGATCCTGGCCGACCACTGCTGTGGCACCTGGATATCGGCCAGGTCTTCATGTATCTCGGCGGCTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTc                                                                                                                             <  2:224142/136‑1 (MQ=255)
                          aCTGCTGTGGCACCTGGATATCGGCCAGGTCTTCATGTATCTCGGCGGCTTCGCCTTTTTGCACTAACGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACAACGTTTcgcccgcc                                                                                                           >  1:142454/1‑136 (MQ=255)
                          aCTGCTGTGGCACCTGGATATCGGCCACGTCTTCATGTATCTCGGCGGCTTCGCCGTTTTGCACTATCGCATCGAAATAGCCGATGGCACCAGCAGCCGGACGCCAGTGCAGCGCGTCGACATCGTTTcgcccgcc                                                                                                           >  1:210110/1‑136 (MQ=255)
                                    cACCTGGATATCGGCCAGGTCTTCATGTATCTCGGCGGCTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGAcc                                                                                                 >  1:444321/1‑136 (MQ=255)
                                                    aGGTCTTCATGTATCTCGGCGGCTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCttt                                                                                 >  1:577090/1‑136 (MQ=255)
                                                    aGGTCTTCATGTATCTCGGCGGCTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCttt                                                                                 >  1:7378/1‑136 (MQ=255)
                                                       tCTTCATGTATCTCGGCGGCTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCCTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCt                                                                              >  2:586499/1‑136 (MQ=255)
                                                            aTGTATCTCGGCGGCTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTggcgg                                                                         <  2:176482/136‑1 (MQ=255)
                                                              gTATCTCGGCGGCTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGgcggcg                                                                       <  2:393726/136‑1 (MQ=255)
                                                                    cggcggCTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGt                                                                 >  1:152095/1‑136 (MQ=255)
                                                                      gcggcTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTAt                                                               <  2:152095/136‑1 (MQ=255)
                                                                       cggcTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTACAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATc                                                              <  2:55337/136‑1 (MQ=255)
                                                                       cggcTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTACAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATc                                                              <  2:58598/136‑1 (MQ=255)
                                                                                 tttttGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGa                                                    <  2:7378/136‑1 (MQ=255)
                                                                                     tGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGa                                                <  1:88881/136‑1 (MQ=255)
                                                                                      gCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGAt                                               <  2:47480/136‑1 (MQ=255)
                                                                                         cTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGgc                                            <  2:347495/136‑1 (MQ=255)
                                                                                         cTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGgc                                            <  1:305320/136‑1 (MQ=255)
                                                                                                    aaTTCGCCGATGGCACCAGCAGCCGGGCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGGCGCGCTGGATcc                                 <  2:553185/136‑1 (MQ=255)
                                                                                                       tCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGGCGCGCTGGATCCtga                              <  2:495155/136‑1 (MQ=255)
                                                                                                         gCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGGCGCGCTGGATCCtgatg                            >  2:594885/1‑136 (MQ=255)
                                                                                                            gATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGGCGCGCTGGATCCTGATGACg                         >  1:124396/1‑136 (MQ=255)
                                                                                                            gATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGGCGCGCTGGATCCTGATGACg                         >  1:626733/1‑136 (MQ=255)
                                                                                                             aTGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTggcgg                                                                         <  1:513526/87‑1 (MQ=255)
                                                                                                             aTGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTggcgg                                                                         >  2:513526/1‑87 (MQ=255)
                                                                                                                 cACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGACCGCCGCCGGCGACCCACCGCCCGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCCACGGATGGCGCGCAGGATCCTGATGACGAAAcc                    >  1:553810/1‑136 (MQ=255)
                                                                                                                      gcagcCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGGCGCGCTGGATCCTGATGACGAAACCTATcg               <  2:394743/136‑1 (MQ=255)
                                                                                                                      gcagcCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGGCGCGCTGGATCCTGATGACGAAACCTATcg               <  1:331384/136‑1 (MQ=255)
                                                                                                                        agcCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGGCGCGCTGGATCCTGATGACGAAACCTATcgcg             <  1:142938/136‑1 (MQ=255)
                                                                                                                             gTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTCTCCCCGGGGCGACGGATGGCGCGCTGGAGCCTGCTGCCGCCACCTATCGCGCCggg        >  1:328212/1‑135 (MQ=255)
                                                                                                                             gTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGGCGCGCTGGATCCTGATGACGAAACCTATCGCGCCGGc        >  1:188043/1‑136 (MQ=255)
                                                                                                                              tCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGGCGCGCTGGATCCTGATGACGAAACCTATCGCGCCGGcc       >  1:203839/1‑136 (MQ=255)
                                                                                                                                 ccAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGGCGCGCTGGATCCTGATGACGAAACCTATCGCGCCGGCCTTc    <  2:668904/136‑1 (MQ=255)
                                                                                                                                   aGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGGCGCGCTGGATCCTGATGACGAAACCTATCGCGCCGGCCTTCtg  <  2:467064/136‑1 (MQ=255)
                                                                                                                                   aGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGGCGCGCTGGATCCTGATGACGAAACCTATCGCGCCGGCCTTCtg  <  1:594885/136‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                    
GTGCAGGAGTCTGATCCTGGCCGACCACTGCTGTGGCACCTGGATATCGGCCAGGTCTTCATGTATCTCGGCGGCTTCGCCTTTTTGCACTATCGCATCGAATTCGCCGATGGCACCAGCAGCCGGTCGCCAGTGCAGCGCTTCGACATCGTTTCGCCCGCCGCCGGCGACCCACCGCCGGCACCTTTGCTGGCGGCGCCACGTATCCCCGGGGCGACGGATGGCGCGCTGGATCCTGATGACGAAACCTATCGCGCCGGCCTTCTG  >  NC_002947/2120025‑2120291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑