| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,247,856 | C→T | 19.4% | A146V (GCC→GTC) | PP_1978 → | TetR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,247,856 | 0 | C | T | 19.4% | 44.4 / 3.9 | 22 | A146V (GCC→GTC) | PP_1978 | TetR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (7/10); new base T (2/2); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGAGCACATCGAGTTGATGACTGCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGGCCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCTGTCTGACCGTATTCTGCGCGCCGCCAAATCGGCGGTGCTTTAACCCTGGAGCATGGCGATGACTACCCTG > NC_002947/2247723‑2247986 | gCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGAGCACATCGAGTTGATGACTGCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGGCCg < 2:491234/136‑1 (MQ=255) tGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGAGCACATCGAGTTGATGACTGCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGGCCGAcc > 2:407526/1‑136 (MQ=255) gCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGAGCACATCGAGTTGATGACTGCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGGCCGACCTGGcc > 1:510179/1‑136 (MQ=255) gCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGAGCACATCGAGTTGATGACTGCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGGCCGACCTGGCCCTGATGGTTGGGGGgct > 2:441211/1‑136 (MQ=255) gCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGAGCACATCGAGTTGATGACTGCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGGCCGACCTGGCCCTGATGGTTGGGGGgctggcgctg > 1:115086/1‑136 (MQ=255) cgcCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGAGCACATCGAGTTGATGACTGCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGGCCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGccc < 1:304031/136‑1 (MQ=255) ttCCGCGAGGTGCTGGCCGAGCACATCGAGTTGATGACTGCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGGCCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCa < 1:407526/136‑1 (MQ=255) tggTGCTGGCCGAGCACATCGAGTTGATGACTGCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGGCCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCt < 1:348140/135‑1 (MQ=255) cggTGCTGGCCGAGCACATCGAGTTGATGACTGCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGGCCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCt < 1:275146/135‑1 (MQ=255) tGCTGGCCGAGCACATCGAGTTGATGACTGCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGGCCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCTGTc < 1:441211/136‑1 (MQ=255) caTCGAGTTGATGACTGCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGCACGACCTGGCCCTGATGGTTGTGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCTGTCTGACCGTATTCTg > 2:164599/1‑136 (MQ=255) cTGCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGGCCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCTGTCTGACCGTATTCt < 1:147625/121‑1 (MQ=255) cTGCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGGCCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCTGTCTGACCGTATTCt > 2:147625/1‑121 (MQ=255) gCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGGCCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCTGTCTGACCGTATTCTGCGCGCCGCCAAATcgg < 2:510179/136‑1 (MQ=255) gACCGCCCTGAGGATGCCGACAAAGCGCTGGTCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCTGTCTGACCGTATTCTGCGCGCCGCCAAATCGGCGGTGCTTTAAc > 2:238481/1‑136 (MQ=255) gACCGCCCTGAGGATGCCGACAAAGCGCTGGTCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCTGTCTGACCGTATTCTGCGCGCCGCCAAATCGGCGGTGCTTTAAc > 2:241570/1‑136 (MQ=255) aGGATGCCGACAAAGCGCTGGTCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCTGTCTGACCGTATTCTGCGCGCCGCCAAATCGGCGGTGCTTTAACCCTGGAGCAt < 1:241570/136‑1 (MQ=255) aGGATGCCGACAAAGCGCTGGTCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCTGTCTGACCGTATTCTGCGCGCCGCCAAATCGGCGGTGCTTTAACCCTGGAGCAt < 1:238481/136‑1 (MQ=255) gACAAAGCGCTGGCCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCTGTCTGACCGTATTCTGCGCGCCGCCAAATCGGCGGTGCTTTAACCCTGGAGCAt < 1:55409/128‑1 (MQ=255) gACAAAGCGCTGGCCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCTGTCTGACCGTATTCTGCGCGCCGCCAAATCGGCGGTGCTTTAACCCTGGAGCAt > 2:55409/1‑128 (MQ=255) gACAAAGCGCTGGCCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCTGTCTGACCGTATTCTGCGCGCCGCCAAATCGGCGGTGCTTTAACCCTGGAGCATGGCGATGa > 1:386103/1‑136 (MQ=255) gcTGGCCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCTGTCTGACCGTATTCTGCGCGCCGCCAAATCGGCGGTGCTTTAACCCTGGAGCATGGCGATGACTACCCTg < 1:463679/136‑1 (MQ=255) | GCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGAGCACATCGAGTTGATGACTGCCGCGATGGTCGACCGCCCTGAGGATGCCGACAAAGCGCTGGCCGACCTGGCCCTGATGGTTGGGGGGCTGGCGCTGGCCCGGGCCTTGGGGACCAGTGACCTGTCTGACCGTATTCTGCGCGCCGCCAAATCGGCGGTGCTTTAACCCTGGAGCATGGCGATGACTACCCTG > NC_002947/2247723‑2247986 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |