Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,505,668 C→A 9.3% D265Y (GAT→TAT)  PP_2194 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,505,6680CA 9.3% 147.0 / 2.5 43D265Y (GAT→TAT) PP_2194LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (15/24);  new base A (2/2);  total (17/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

CTTACTGATTGTGGTGGCCTGAACGGGCCTGTTCAGGCAAACGCCGCCAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGTCAGCACGCTTTCGATCTGCTCCGCCACTGCCG  >  NC_002947/2505538‑2505799
                                                                                                                                  |                                                                                                                                   
cTTACTGATTGTGGTGGCCTGAACGGGCCTGTTCAGGCAAACGCCGCCAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGca                                                                                                                                >  1:330295/1‑135 (MQ=255)
cTTACTGATTGTGGTGGCCTGAACGGGCCTGTTCAGGCAAACGCCGCCAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCt                                                                                                                                >  2:56931/1‑136 (MQ=255)
cTTACTGATTGTGGTGGCCTGAACGGGCCTGTTCAGGCAAACGCCGCCAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCt                                                                                                                                >  2:114011/1‑136 (MQ=255)
             gtggCCTGAACGGGCCTGTTCAGGCAAACTCCGCCAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAgcgc                                                                                                                   >  2:144548/1‑136 (MQ=255)
                    gAACGGGCCTGTTCAGGCAAACGCCGCCAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAAc                                                                                                            >  1:128569/1‑136 (MQ=255)
                           ccTGTTCAGGCAAACGCCGCCAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCt                                                                                                     <  2:50567/136‑1 (MQ=255)
                           ccTGTTCAGGCAAACGCCGCCAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCt                                                                                                     <  1:56931/136‑1 (MQ=255)
                             tGTTCGGGCAAACGCCGCCAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATAAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCg                                                                                                   >  1:570855/1‑136 (MQ=255)
                             tGTTCAGGCAAACGCCGCCAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCg                                                                                                   >  2:27578/1‑136 (MQ=255)
                             tGTTCAGGCAAACGCCGCCAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATAAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCg                                                                                                   >  1:636551/1‑136 (MQ=255)
                                  aGGCAAACGCCGCCAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTc                                                                                              >  1:534896/1‑136 (MQ=255)
                                     cAAACGCCGCCAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCa                                                                                           <  2:407762/136‑1 (MQ=255)
                                         cgccgcCAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGcc                                                                                       <  2:112959/136‑1 (MQ=255)
                                          gccgccAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCt                                                                                      <  1:518607/136‑1 (MQ=255)
                                                            tcAAATACATGGGCTGCGTCGCCAACCCGCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAAtggcatggca                                                                    <  2:654931/135‑1 (MQ=255)
                                                            gcAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAAtggcatggca                                                                    <  2:403479/136‑1 (MQ=255)
                                                            gcAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAAtggcatggca                                                                    <  2:128569/136‑1 (MQ=255)
                                                              aaaTACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAg                                                                  >  1:508266/1‑136 (MQ=255)
                                                               aaTACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGt                                                                 >  1:194636/1‑136 (MQ=255)
                                                                 tACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAg                                                               <  1:114011/136‑1 (MQ=255)
                                                                        gCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGt                                                                                                                          <  2:309727/70‑1 (MQ=255)
                                                                        gCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGt                                                                                                                          >  1:309727/1‑70 (MQ=255)
                                                                           gCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATAtgct                                                     <  2:330295/136‑1 (MQ=255)
                                                                               cGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCt                                                                                      <  2:544649/99‑1 (MQ=255)
                                                                               cGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCt                                                                                      >  1:544649/1‑99 (MQ=255)
                                                                               cGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGccc                                                 <  1:497253/136‑1 (MQ=255)
                                                                                 ccAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCcag                                               <  1:144548/136‑1 (MQ=255)
                                                                                      ccccTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCcagacaga                                          <  2:160625/136‑1 (MQ=255)
                                                                                         cTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATg                                       >  1:177315/1‑136 (MQ=255)
                                                                                              cccGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGt                                  <  1:75002/136‑1 (MQ=255)
                                                                                              cccGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGt                                  <  2:122895/136‑1 (MQ=255)
                                                                                               ccGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGTc                                 >  2:251093/1‑136 (MQ=255)
                                                                                                cGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGTCa                                <  2:452337/136‑1 (MQ=255)
                                                                                                cGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATAAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGTCa                                <  2:570855/136‑1 (MQ=255)
                                                                                                cGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATAAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGTCa                                <  2:636551/136‑1 (MQ=255)
                                                                                                     ccGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGTCa                                >  1:57739/1‑131 (MQ=255)
                                                                                                     ccGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGTCa                                <  2:57739/131‑1 (MQ=255)
                                                                                                     ccGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGTCAGCACg                           <  2:624946/136‑1 (MQ=255)
                                                                                                                tGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGTACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGTCAGCACGCTTTCGATCTg                >  1:245044/1‑136 (MQ=255)
                                                                                                                        aCCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGTCAGCACGCTTTCGATCTGCTCCGCCa        <  1:97985/136‑1 (MQ=255)
                                                                                                                        aCCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGTCAGCACGCTTTCGATCTGCTCCGCCa        <  2:508266/136‑1 (MQ=255)
                                                                                                                              aaaTCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGTACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGTCAGCACGCTTTCGATCTGCTCCGCCACTGCCg  <  2:245044/136‑1 (MQ=255)
                                                                                                                              aaaTCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGTCAGCACGCTTTCGATCTGCTCCGCCACTGCCg  <  1:333773/136‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                   
CTTACTGATTGTGGTGGCCTGAACGGGCCTGTTCAGGCAAACGCCGCCAGCAAATCCTGCGCAAATACATGGGCTGCCTCGCCAACCCCCTGTGCCCGGTGCCGGGCCAGGGTGAACGGCACCGCAAAATCAAGCTCAGGGTCCAGCGCCCATAACAGCCCCTCGGCCTCCCAGGCCTGTGCACAATGGCATGGCAAGTAGCCAATATGCTGCCCAGACAGAATGAACGTCAGCACGCTTTCGATCTGCTCCGCCACTGCCG  >  NC_002947/2505538‑2505799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑