Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,902,452 T→A 11.4% L260Q (CTG→CAG)  PP_2551 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,902,4520TA11.4% 95.6 / 3.6 35L260Q (CTG→CAG) PP_2551LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (15/16);  new base A (2/2);  total (17/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAGGCCTCGGCCGAGCACCGCTGGTTGCGTAACCGGGTATTCGAGTTGTGGCGCCAGTCGCAGT  >  NC_002947/2902319‑2902583
                                                                                                                                     |                                                                                                                                   
gCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCACCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGAcctgg                                                                                                                                   >  2:328822/1‑136 (MQ=255)
    aGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGAcctggcctg                                                                                                                               >  2:524477/1‑136 (MQ=255)
         cTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGAc                                                                                                                          >  1:187648/1‑136 (MQ=255)
         cTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGAc                                                                                                                          >  2:315976/1‑136 (MQ=255)
         cTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGAc                                                                                                                          >  1:605058/1‑136 (MQ=255)
                       cAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGACAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATc                                                                                                            <  1:315976/136‑1 (MQ=255)
                                aGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATc                                                                                                            >  2:310955/1‑127 (MQ=255)
                                aGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATc                                                                                                            <  1:310955/127‑1 (MQ=255)
                                aGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCAcagc                                                                                                   <  2:643461/136‑1 (MQ=255)
                                aGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCAcagc                                                                                                   <  2:587668/136‑1 (MQ=255)
                                aGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCACCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCAcagc                                                                                                   <  1:328822/136‑1 (MQ=255)
                                       ttcttcTGCGCCACGGTACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCt                                                                                            >  1:160283/1‑136 (MQ=255)
                                          ttctGCGCCACGGTACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACt                                                                                         <  2:178586/136‑1 (MQ=255)
                                                       tACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCa                                                                            >  2:172906/1‑136 (MQ=255)
                                                        aCAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCAc                                                                           <  1:215010/136‑1 (MQ=255)
                                                                ttGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCt                                                                   <  1:325368/136‑1 (MQ=255)
                                                                  gCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCAGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGc                                                                 >  2:329115/1‑136 (MQ=255)
                                                                  gCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCAGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGc                                                                 >  2:325732/1‑136 (MQ=255)
                                                                    cagcagTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCAcagc                                                                                                   <  2:256916/100‑1 (MQ=255)
                                                                    cagcagTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCAcagc                                                                                                   >  1:256916/1‑100 (MQ=255)
                                                                    cagcagTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCac                                                               >  1:382433/1‑135 (MQ=255)
                                                                    cagcagTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCCACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCCCCAGCac                                                               >  1:359624/1‑135 (MQ=255)
                                                                     cgcagTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAgg                                                              <  1:298223/135‑1 (MQ=255)
                                                                     agcagTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAgg                                                              <  2:160283/136‑1 (MQ=255)
                                                                             ggCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCCGCGCCTGCAGGCCTCGGcc                                                      >  1:102389/1‑136 (MQ=255)
                                                                                     ccACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAGGCCTCGGCCGAGCACCg                                              >  1:434198/1‑136 (MQ=255)
                                                                                                gAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGTGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAGGCCTCGGCCGAGCACCGCTGGTTGCGTa                                   <  2:613473/136‑1 (MQ=255)
                                                                                                gAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAGGCCTCGGCCGAGCACCGCTGGTTGCGTa                                   <  2:476051/136‑1 (MQ=255)
                                                                                                gAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAGGCCTCGGCCGAGCACCGCTGGTTGCGTa                                   <  1:658882/136‑1 (MQ=255)
                                                                                                gAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCAGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAGGCCTCGGCCGAGCACCGCTGGTTGCGTa                                   <  1:325732/136‑1 (MQ=255)
                                                                                                gAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCAGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAGGCCTCGGCCGAGCACCGCTGGTTGCGTa                                   <  1:329115/136‑1 (MQ=255)
                                                                                                        cGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAGGCCTCGGCCGAGCACCGCTGGTTGCGTAACCGGGTa                           >  1:277677/1‑136 (MQ=255)
                                                                                                         gCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAGGCCTCGGCCGAGCACCGCTGGTTGCGTAACCGGGTAt                          >  2:29944/1‑136 (MQ=255)
                                                                                                         gCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAGGCCTCGGCCGAGCACCGCTGGTTGCGTAACCGGGTAt                          >  2:569134/1‑136 (MQ=255)
                                                                                                                  cTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAGGCCTCGGCCGAGCACCGCTGGTTGCGTAACCGGGTATTCGAGTtgt                 <  1:498854/136‑1 (MQ=255)
                                                                                                                        cGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAGGCCTCGGCCGAGCACCGCTGGTTGCGTAACCGGGTATTCGAGTTGTGGCGcc           >  2:473122/1‑136 (MQ=255)
                                                                                                                                 gACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAGGCCTCGGCCGAGCACCGCTGGTTGCGTAACCGGGTATTCGAGTTGTGGCGCCAGTCGCAGt  <  1:333038/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
GCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGTCAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCTGCAGGCCTCGGCCGAGCACCGCTGGTTGCGTAACCGGGTATTCGAGTTGTGGCGCCAGTCGCAGT  >  NC_002947/2902319‑2902583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑