Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,035,802 C→A 10.0% A39A (GCC→GCA)  mgtA → ATP‑dependent magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,035,8020CA10.0% 134.4 / 2.7 40A39A (GCC→GCA) mgtAATP‑dependent magnesium transporter
Reads supporting (aligned to +/- strand):  ref base C (22/14);  new base A (2/2);  total (24/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

GTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAACC  >  NC_002947/3035673‑3035936
                                                                                                                                 |                                                                                                                                      
gTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGt                                                                                                                                  <  1:174128/136‑1 (MQ=255)
gTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGt                                                                                                                                  <  1:577413/136‑1 (MQ=255)
  tCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACAAGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGACTGTCCACCCGCGCCGCCCGTGa                                                                                                                                >  2:140713/1‑136 (MQ=255)
                   cGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTgg                                                                                                               >  1:569376/1‑136 (MQ=255)
                             tCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCAccca                                                                                                     >  1:264308/1‑135 (MQ=255)
                                 gccgccGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGcc                                                                                                 >  2:575409/1‑136 (MQ=255)
                                 gccgccGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGcc                                                                                                 >  2:61194/1‑136 (MQ=255)
                                   cgccgcAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGcc                                                                                                 >  1:382720/1‑134 (MQ=255)
                                   cgccgcAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGcc                                                                                                 <  2:382720/134‑1 (MQ=255)
                                              cAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCa                                                                                    >  1:103041/1‑136 (MQ=255)
                                                ggAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGc                                                                                  <  1:296881/136‑1 (MQ=255)
                                                       aGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAgg                                                                           <  2:603390/136‑1 (MQ=255)
                                                                    tCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATg                                                                                       >  2:33155/1‑111 (MQ=255)
                                                                    tCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATg                                                                                       <  1:33155/111‑1 (MQ=255)
                                                                    tCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCa                                                              >  1:170505/1‑136 (MQ=255)
                                                                    tCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAAT‑GCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGACCAGGGCTTGACCGAGCAc                                                             >  2:665186/1‑136 (MQ=255)
                                                                    tCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAAT‑GCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCAc                                                             >  2:668091/1‑136 (MQ=255)
                                                                      aaaTACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACg                                                            >  2:595883/1‑136 (MQ=255)
                                                                       aaTACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGa                                                           <  1:66448/136‑1 (MQ=255)
                                                                          aCCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCGACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAgc                                                        <  1:568825/136‑1 (MQ=255)
                                                                            cGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAgccg                                                      <  2:170505/136‑1 (MQ=255)
                                                                                 cacCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAg                                                 >  1:271503/1‑136 (MQ=255)
                                                                                       aTCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGaa                                                                                                                               >  2:156445/1‑52 (MQ=255)
                                                                                       aTCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGaa                                                                                                                               <  1:156445/52‑1 (MQ=255)
                                                                                       aTCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCACCCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTg                                           >  2:654698/1‑136 (MQ=255)
                                                                                         cGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGt                                         >  1:663047/1‑136 (MQ=255)
                                                                                         cGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCAGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCATCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGt                                         >  1:45699/1‑136 (MQ=255)
                                                                                         cGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCAGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCATCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGt                                         >  1:105973/1‑136 (MQ=255)
                                                                                         aatacAAGGCCGAACGCGAGACCCGCCTGTAAACAAGAGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGt                                         >  1:541320/5‑136 (MQ=255)
                                                                                            ccAAGGCCGAACGCGCGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGCTGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTgcg                                      <  2:230078/136‑1 (MQ=255)
                                                                                            ccAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTgcg                                      <  2:674484/136‑1 (MQ=255)
                                                                                            ccAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTgcg                                      <  2:151876/136‑1 (MQ=255)
                                                                                             cAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCAGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCATCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTgcgc                                     <  2:45699/136‑1 (MQ=255)
                                                                                             cAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCAGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCATCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTgcgc                                     <  2:105973/136‑1 (MQ=255)
                                                                                                   cGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTGCGCGATggg                               >  2:333488/1‑136 (MQ=255)
                                                                                                        gcgAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTGCGCGATGGGGCCaa                          >  1:598918/1‑136 (MQ=255)
                                                                                                          gagaCCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTGCGCGATGGGGCCAAcc                        <  2:643826/136‑1 (MQ=255)
                                                                                                                     gTCCACCCGCGCCGCCCGTGAAGCTCGCAATGGACTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTCGTGCGCGATGGGGCCAACCAGGTTGCCCAc             >  1:552499/1‑136 (MQ=255)
                                                                                                                         aCCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGAcc         >  1:494218/1‑136 (MQ=255)
                                                                                                                           ccGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGCCCCg       >  1:581469/1‑136 (MQ=255)
                                                                                                                              cgccgcCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCaa    >  1:634529/1‑136 (MQ=255)
                                                                                                                                ccgccCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCTACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGACAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAAcc  <  1:140713/136‑1 (MQ=255)
                                                                                                                                ccgccCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAAcc  >  1:308432/1‑136 (MQ=255)
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GTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAGCGAACAGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAACC  >  NC_002947/3035673‑3035936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑