| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,072,445 | G→A | 19.0% | K164K (AAG→AAA) | qedH‑II → | quinoprotein ethanol dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,072,445 | 0 | G | A | 19.0% | 42.0 / 5.0 | 21 | K164K (AAG→AAA) | qedH‑II | quinoprotein ethanol dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (10/7); new base A (2/2); total (12/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01 | |||||||||||
CTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGC > NC_002947/3072346‑3072576 | ctgcGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaagaa < 2:440873/136‑1 (MQ=255) tgcGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaagaag > 2:410241/1‑136 (MQ=255) tgcGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaagaag > 2:177023/1‑136 (MQ=255) gcGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAgg > 1:574169/1‑136 (MQ=255) ggCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAAGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCa > 2:531339/1‑136 (MQ=255) ggCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAAGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCa > 2:528019/1‑136 (MQ=255) ggTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAg > 2:379432/1‑136 (MQ=255) tgtTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACcac > 1:347101/1‑86 (MQ=255) tgtTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACcac < 2:347101/86‑1 (MQ=255) tcttcGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGAt > 2:65844/1‑136 (MQ=255) gCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCg > 2:166938/1‑136 (MQ=255) tGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCgtggt < 1:166938/136‑1 (MQ=255) gAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCgtggtg < 2:574169/136‑1 (MQ=255) aacaacGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGtggtgg < 1:25037/136‑1 (MQ=255) aaCAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGtggtgg < 1:78780/136‑1 (MQ=255) aaCAAGGACACCGGCAAAGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGtggtgg < 1:531339/136‑1 (MQ=255) aaCAAGGACACCGGCAAAGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGtggtgg < 1:528019/136‑1 (MQ=255) cacCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATc > 1:500794/1‑136 (MQ=255) gCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGc < 1:626774/136‑1 (MQ=255) gCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGc > 2:57704/1‑136 (MQ=255) gCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGc > 2:626774/1‑136 (MQ=255) | CTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGC > NC_002947/3072346‑3072576 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |