| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,234,188 | 0 | G | C | 53.8% | ‑0.8 / 13.2 | 13 | intergenic (‑133/+43) | PP_4606/PP_4607 | ferric siderophore receptor/transmembrane sensor |
| Reads supporting (aligned to +/- strand): ref base G (2/4); new base C (7/0); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.44e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑T‑AAACCCGCCCCTACGGGGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCA > NC_002947/5234057‑5234280 | gACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑T‑TAAACcccccc > 2:308913/1‑136 (MQ=255) ccGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑T‑AAACCCGCCCCt < 2:215067/135‑1 (MQ=255) aCGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑T‑AAACCCGCCCCt < 1:3325/136‑1 (MQ=255) attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑T‑TAAACCCCCCCAAAGGGGGGTTTAGCCggggag > 2:230268/1‑131 (MQ=255) caatcGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑T‑TAACCCCCCCCGAAGGGGGGGTTTCCCGTGAAGAGGCggggccac > 2:278804/1‑131 (MQ=255) cGCACTTTCACTCATTACACGGATGCCACGTGCTGTAACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑T‑AAACCCGCCCCTACGGGGGGTTTACCCGTGATGAGGCTGGCACAGGCaa < 1:21592/136‑1 (MQ=255) tCACTCATTACACGGATGCCACGTGCTGTAACCTCACCTGTGCCTGGAAATATTTTTGCTGGGGCGGCCTCTTCGC‑GGG‑T‑AACCCCGCCCCTACGGGGGGTTTACCCGTTAAGTGGCTGGCACAGGCAACTACCGa < 2:309766/136‑1 (MQ=39) acacGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAAACCCCCCCAAACGGGGGTTTTACCC‑TGAAGAGGCAGGCACAAGCAACAAACGAAccccggta > 1:241780/1‑130 (MQ=255) acGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGTT‑AAACCCCCCCCACGGGGGGGTTTACCCG‑GCAGATGCGGGGCCACGCAACAAACCAACCACGGGCaa > 1:318693/1‑136 (MQ=255) cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGTT‑AAACCCCCCCAAACGGGGGGTTTA‑CCGGGAAGAGGCTGGCCCAGGCCACAACCCAACCCCGGGCAAAAGgcccc > 1:202293/1‑135 (MQ=255) ttACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑T‑AAAACCCCCCCAACGGGGGGGTTACCCGTGAAGAGGCGGGCACAGGCCACAACCGAACCCGGGTCAAACGCCCCAACACCGTCt > 2:198771/1‑136 (MQ=255) gtgCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑T‑AAACCCGCCCCTACGGGGGTTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGaaaaa > 2:199827/1‑136 (MQ=255) cTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGG‑T‑AAACCCCCCCCACCGGGGGGTTTACCCGGGAGAGGGGGGGCCCCGGCACGAACCCAACCCGGGGAAACCCCCCCACCCCGGGGGGCCGGGAAAAAccc > 2:294792/1‑136 (MQ=255) cTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑T‑AAACCCGCCCCAACGGGGGGTTTTCCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAAAACGGTCTGCCGTGAAAAACCCa > 1:85419/1‑136 (MQ=255) | GACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑T‑AAACCCGCCCCTACGGGGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCA > NC_002947/5234057‑5234280 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |