Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947195,6340CT26.1% 35.1 / 11.2 24T380T (ACC→ACT) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (8/9);  new base T (3/3);  total (12/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGG  >  NC_002947/195501‑195767
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cGGCGAATGACGTCAACAACAACGGTTCGACCGTCAGCGTCACCAGCTACAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCCAAACCCCCCCCGGCTCACACCACGACCAACGACTCGGTCGAcaccactac                                                                                                                                     <  2:555742/136‑1 (MQ=11)
  gCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACcg                                                                                                                                   >  2:25520/1‑136 (MQ=40)
    cAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAAGCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACAACcgcg                                                                                                                                 >  2:374106/1‑136 (MQ=12)
                     acGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGccc                                                                                                                >  1:446349/1‑136 (MQ=17)
                              ccGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTTACGGCTAGCCCGTCGGTCaa                                                                                                       >  1:446232/1‑135 (MQ=255)
                                    gCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAgg                                                                                                 >  1:466524/1‑136 (MQ=37)
                                    gCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAgg                                                                                                 >  1:4924/1‑136 (MQ=37)
                                        aaCCATCGAGAACGCCACAGGCGTTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACTATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                                                             <  2:443511/135‑1 (MQ=255)
                                              cGAGAACGCCACGGGCGTTAATTTCGAGCAACTGACCCCGAATCCGCCCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATc                                                                                       <  2:309860/136‑1 (MQ=255)
                                              cGAGAACGCCACCGGCGTTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCGCAGACCACTATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATc                                                                                       <  1:572564/136‑1 (MQ=255)
                                                ataaCGCCACGGGCGTTATTTTCGACCAACTGACCCCGAATCCCCCCCGGGCTCAGACCACGAACAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATcgc                                                                                     <  1:216378/134‑3 (MQ=11)
                                                ataaCGCCACCGGCGTTAATTTCGAGCAACTGACCCCTAATCCGACCCCGGCTCATACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc                                                                                     <  2:524023/134‑1 (MQ=255)
                                                agaACGCCCCCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc                                                                                     <  2:440901/136‑1 (MQ=37)
                                                agaACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc                                                                                     <  1:25520/136‑1 (MQ=37)
                                                agaACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc                                                                                     <  2:158645/136‑1 (MQ=37)
                                                acaaCGCCACCGGCGTAAATTTCGACCAACTGACCCCTAATCCCACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc                                                                                     <  2:248729/134‑1 (MQ=11)
                                                  aaCGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAcat                                                                                   >  1:386094/1‑134 (MQ=12)
                                                      ccACAGGCTTTATTTTCTAGCAACTTACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTacac                                                                               <  1:408305/136‑1 (MQ=11)
                                                                  aTTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAg                                                                   >  2:546680/1‑136 (MQ=37)
                                                                           aaCTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGCGCAACCCTGc                                                          >  1:220916/1‑136 (MQ=255)
                                                                           aaCTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCAGc                                                          >  1:217965/1‑136 (MQ=255)
                                                                                           gACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCAGACCGCGAGACCGTCGCTCACCGCCCGCGGCGAGAGAACTAAGACCGTGACCCTGAGCAACCCTGCCAAAACGCCGGTAAcc                                          >  1:640694/1‑136 (MQ=11)
                                                                                                    tCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTg                                 <  2:446349/136‑1 (MQ=37)
                                                                                                            cGACCAACCACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACgg                         <  2:472003/136‑1 (MQ=255)
                                                                                                                     aCTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGAt                >  2:536103/1‑136 (MQ=40)
                                                                                                                            cgatgccaccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGtt         >  2:111896/6‑136 (MQ=2)
                                                                                                                                 ccaccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGc    >  1:171155/1‑136 (MQ=21)
                                                                                                                                   cccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg  <  2:323783/135‑1 (MQ=40)
                                                                                                                                   accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg  <  2:466524/136‑1 (MQ=40)
                                                                                                                                   accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg  <  2:4924/136‑1 (MQ=40)
                                                                                                                                   accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg  <  1:546680/136‑1 (MQ=40)
                                                                                                                                     |                                                                                                                                     
CGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGG  >  NC_002947/195501‑195767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑