Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,092,4450TG43.8% 9.4 / 42.1 32intergenic (+266/+652)PP_1865/aroHtransposase/3‑deoxy‑7‑phosphoheptulonate synthase
Reads supporting (aligned to +/- strand):  ref base T (7/11);  new base G (13/1);  total (20/12)
Fisher's exact test for biased strand distribution p-value = 2.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAAC  >  NC_002947/2092316‑2092570
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aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATGTCCCTCCGCAATGCCTCCACTCGGCCTCCGGCAGTCGCAATCTGCGTCGGCTGGGCGTCCGCGCGCGTAGAAGCAAAAGCCAAAg                                                                                                                         >  2:510665/1‑136 (MQ=14)
aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAg                                                                                                                         >  2:306446/1‑136 (MQ=255)
aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAAAGCCAAAg                                                                                                                         >  2:445142/1‑136 (MQ=255)
aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAAAGCCAAAg                                                                                                                         >  2:412008/1‑136 (MQ=255)
aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAAAGCCAAAg                                                                                                                         >  2:376931/1‑136 (MQ=255)
aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAAAGCCAAAg                                                                                                                         >  1:400517/1‑136 (MQ=255)
aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAAAGCCAAAg                                                                                                                         >  2:340766/1‑136 (MQ=255)
aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAAAGCCAAAg                                                                                                                         >  1:480191/1‑136 (MQ=255)
aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAAAGCCAAAg                                                                                                                         >  2:142643/1‑136 (MQ=255)
aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAAAGCCAAAg                                                                                                                         >  2:115376/1‑136 (MQ=255)
aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTAGAAGCAAAAGCCAAAg                                                                                                                         >  1:7505/1‑136 (MQ=255)
aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCAAGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAAAGCCAAAg                                                                                                                         >  2:375788/1‑136 (MQ=255)
 ggCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAAAGCCAAAGc                                                                                                                        >  2:619248/1‑136 (MQ=255)
 ggCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACAACCGCGCGCTTAGAAGCAAAAGCCAAAGc                                                                                                                        >  2:388210/1‑136 (MQ=255)
          catGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAAAGCCAAAGCaagagcaag                                                                                                               <  1:142643/136‑6 (MQ=11)
                     aGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATc                                                                                                    >  1:130497/1‑136 (MQ=255)
                     aGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATc                                                                                                    >  1:173147/1‑136 (MQ=255)
                        gcTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATcggc                                                                                                 <  2:225855/136‑1 (MQ=255)
                          ttGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATcggccg                                                                                               <  2:158658/136‑1 (MQ=255)
                          ttGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATcggccg                                                                                               <  2:108287/136‑1 (MQ=255)
                                    tCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGa                                                                                     <  1:330772/136‑1 (MQ=255)
                                     ccATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGAt                                                                                    <  1:306446/136‑1 (MQ=255)
                                     ccATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGAt                                                                                    <  1:380936/136‑1 (MQ=255)
                                                          gCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGccc                                                               >  2:375420/1‑136 (MQ=255)
                                                              tGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCTCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTc                                                           <  1:416042/136‑1 (MQ=255)
                                                                      cTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGAt                                                   >  1:261856/1‑136 (MQ=255)
                                                                      cTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGAt                                                   >  2:603423/1‑136 (MQ=255)
                                                                           cctcctGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGa                                              >  2:439024/1‑136 (MQ=255)
                                                                                                    ccTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCggg                     <  2:130497/136‑2 (MQ=255)
                                                                                                      gggACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCt                   <  1:66097/135‑1 (MQ=255)
                                                                                                                   gcTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGgaa      <  1:457825/136‑1 (MQ=255)
                                                                                                                       aGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAAc  <  1:603423/136‑1 (MQ=255)
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AGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAAC  >  NC_002947/2092316‑2092570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑