| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,167,122 | A→G | N377S (AAC→AGC) | gtsD → | mannose/glucose ABC transporter ATP binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,167,122 | 0 | A | G | 93.8% | 48.2 / ‑3.8 | 16 | N377S (AAC→AGC) | gtsD | mannose/glucose ABC transporter ATP binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (8/7); total (8/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
GGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAACAGCGCAAACGCATCAAGACACTGGGATCGTTGGCCTTGCTTGCACTTGTAGGCAGC > NC_002947/1166991‑1167250 | ggCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAGCGTg < 2:167703/136‑1 (MQ=255) gcgcCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCCACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAGCGTgg < 2:264346/136‑1 (MQ=255) tGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAGCGTGGCTCACTTCAAAGGcc > 2:257670/1‑136 (MQ=255) ccGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAGCGTGGCTCACTTCAAAGGCCGt < 2:228728/136‑1 (MQ=255) cAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGCGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAGCGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATc > 1:100810/1‑136 (MQ=255) ggcccggGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAGCGTGGCTCACTTCAAAGGCCGt < 1:47997/100‑1 (MQ=255) ggcccggGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAGCGTGGCTCACTTCAAAGGCCGt > 2:47997/1‑100 (MQ=255) ggcccggGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAGCGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACg < 1:264562/136‑1 (MQ=255) gggTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAGCGTGGCGCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGCCGCAAAg > 1:21836/1‑136 (MQ=255) tgTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAGCGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAAc > 2:277327/1‑136 (MQ=255) gTACTGGCGCAACCGCAAAGGACAGCGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAACAGCGCAAACGCATCAAGACACTGGGATCGTTGGCcttgc < 2:100810/136‑1 (MQ=255) tGGCGCAACCGCAAAGGACAGCGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAACAGCGCAAACGCATCAAGACACTGGGATCGTTGGCCttgcttgc > 2:154864/1‑136 (MQ=255) cAACCGCAAAGGACAGCGTGGCTCACTTCAAAGGCCGTTGACTCGTCt > 1:186782/1‑48 (MQ=255) cAACCGCAAAGGACAGCGTGGCTCACTTCAAAGGCCGTTGACTCGTCt < 2:186782/48‑1 (MQ=255) gCAAAGGACAGCGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAACAGCGCAAACGCATCAAGACACTGGGATCGTTGGCCTTGCTTGCACTTGTAGgc > 2:131316/1‑136 (MQ=255) aaGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAACAGCGCAAACGCATCAAGACACTGGGATCGTTGGCCTTGCTTGCACTTGTAGgcagc < 1:192341/136‑1 (MQ=255) | GGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAACAGCGCAAACGCATCAAGACACTGGGATCGTTGGCCTTGCTTGCACTTGTAGGCAGC > NC_002947/1166991‑1167250 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |