| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 4,018,201 | G→A | intergenic (+144/‑36) | mvaB → / → PP_mr44 | hydroxymethylglutaryl‑CoA lyase/CrcZ ncRNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,018,201 | 0 | G | A | 94.4% | 56.5 / NA | 18 | intergenic (+144/‑36) | mvaB/PP_mr44 | hydroxymethylglutaryl‑CoA lyase/CrcZ ncRNA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (9/8); minor base T (0/1); total (9/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCACATTGGTGTGGGTGAAGTGTTACCTCCCGCACATTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGGTTTTCAAAA‑GTTGGCACGCACCCTGCTATAT‑CTCTGGTACAACAACAAAAAAAACGCGATACCCAATAAAAACAACAGGTAACGGCTCTGACATAACAAGAACAACACGGCAGAGGCGTAGCAAGC > NC_002947/4018070‑4018327 | tCACATTGGTGTGGGTGAAGTGTTACCTCCCGCACATTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGAtttt > 1:270038/1‑136 (MQ=255) gtgGGTGAAGTGTTACCTCCCGCACATTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAA‑GTTgg > 2:148174/1‑136 (MQ=255) ggTGAAGTGTTACCTCCCGCACATTTCACAGCAAAAAATCGTGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAA‑GTTGgcac < 2:218486/136‑1 (MQ=255) gAAGTGTTACCTCCCGCACATTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAA‑GTTGgcacgca > 1:64688/1‑136 (MQ=255) gAAGTGTTACCTCCCGCACATTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAA‑GTTGgcacgca > 1:108266/1‑136 (MQ=255) ccTCCCGCACATTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAA‑GTTGGCACGCACCCTGCtat < 2:135206/136‑1 (MQ=255) aaTCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAA‑GTTGGCACGCACCCTGCTATAT‑CTCTGGTACAACAACaaaaaaa < 1:23495/136‑1 (MQ=255) aaCAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAA‑GTTGGCACGCACCCTGCTATAT‑CTCTGGTACAACAACAAAAAAAACGCGATa > 1:190422/1‑136 (MQ=255) gCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTAAAAGGATTTTCAAAA‑GTTGGCACGCACCCTGCTATAT‑CTCTGGTACAACAACAAAAAAAACGCGATACCCAATAAAAACAACAg < 1:115752/136‑1 (MQ=255) cAGAATTTCAGGCTGGGTTGTTTCGACAAAAATTCTTAACTCATTAATTTTAAAGGATTTTCAAAA‑GTTGGCACGCACCCTGCTATAT‑CTCTGGTACAACAACAAAAAAAACGCGATACCCAATAAAAACAACAgg < 1:148174/136‑1 (MQ=255) cAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAA‑GTTGGCACGCACCCTGCTATAT‑CTCTGGTACAACAACAAAAAAAACGCGATACCCAATAAAAACAACAgg > 2:267791/1‑136 (MQ=255) aTTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAA‑GTTGGCACGCACCCTGCTATAT‑CTCTGGTACAACAACAAAAAAAACGCGATACCCAATAAAAACAACAGGTAAc < 1:87148/136‑1 (MQ=255) ggtggtGGCAA‑CAAAAATAATAAATTATTAAATTTAAATGTTTTTTAAAATTTTGCCACGCACCGTGAT‑TATGATGTGGT‑CAAAAAAAAAAAAAAACCCTTAACCAATAAAAACAACAGGTAACGGCTCTGACATa < 2:270038/136‑1 (MQ=255) ggtgTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAA‑GTTGGCACGCACCCTGCTATAT‑CTCTGGTACAACAACAAAAAAAACGCGATACCCAATAAAAACAACAGGTAACGGCTCTGACATAAc > 2:259353/1‑136 (MQ=255) cGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAA‑GTTGGCACGCACCCTGCTATAT‑CTCTGGTACAACAACAAAAAAAACGCGATACCCAATAAAAACAACAGGTAACGGCTCTGACATAACAAGaa < 2:190422/136‑1 (MQ=255) aCCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAA‑GTTGGCACGCACCCTGCTATAT‑CTCTGGTACAACAACAAAAAAAACGCGATACCCAATAAAAACAACAGGTAACGGCTCTGACATAACAAGaaca < 1:259353/136‑1 (MQ=255) cTCATTAATTTTAAAGGATTTTCAAAA‑GTTGGCACGCACCCTGCTATAT‑CTCTGGTACAACAACAAAAAAAACGCGATACCCAATAAAAACAACAGGTAACGGCTCTGACATAACAAGAACAACACGGCAGAGGCg > 2:233241/1‑136 (MQ=255) ttttAAAGGATTTTCAAAA‑GTTGGCACGCACCCTGCTATAT‑CTCTGGTACAACAACAAAAAAAACGCGATACCCAATAAAAACAACAGGTAACGGCTCTGACATAACAAGAACAACACGGCAGAGGCGTagcaagc > 1:82089/1‑136 (MQ=255) | TCACATTGGTGTGGGTGAAGTGTTACCTCCCGCACATTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGGTTTTCAAAA‑GTTGGCACGCACCCTGCTATAT‑CTCTGGTACAACAACAAAAAAAACGCGATACCCAATAAAAACAACAGGTAACGGCTCTGACATAACAAGAACAACACGGCAGAGGCGTAGCAAGC > NC_002947/4018070‑4018327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |