BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,163,733	A→G	N304S (AAC→AGC)	gtsA →	mannose/glucose ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,163,733	0	A	G	100.0%	1532.7 / NA	431	N304S (AAC→AGC)	gtsA	mannose/glucose ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (202/229); total (202/229)

Only 100 of 439 total aligned reads displayed.

TGGAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTG  >  NC_002947/1163586‑1163879

tGGAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGACTGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTacagca                                                                                                                                                  >  1:9799388/1‑150 (MQ=255)

  gAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATc                                                                                                                                                >  2:10271743/1‑150 (MQ=255)

  gAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATc                                                                                                                                                >  1:7626603/1‑150 (MQ=255)

  gAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATc                                                                                                                                                <  1:3807902/150‑1 (MQ=255)

   aaGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCg                                                                                                                                               >  2:740389/1‑150 (MQ=255)

    aGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGa                                                                                                                                              >  1:5294926/1‑150 (MQ=255)

     gccgccAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGAc                                                                                                                                             >  2:6453809/1‑150 (MQ=255)

      ccgccAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACt                                                                                                                                            <  2:6278114/150‑1 (MQ=255)

             ggTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTtggt                                                                                                                                     <  2:10042568/150‑1 (MQ=255)

              gTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTtggtg                                                                                                                                    <  1:10071690/150‑1 (MQ=255)

              gTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTtggtg                                                                                                                                    <  2:5979807/150‑1 (MQ=255)

              gTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTtggtg                                                                                                                                    <  2:4147335/150‑1 (MQ=255)

              gTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTtggtg                                                                                                                                    <  1:4771039/150‑1 (MQ=255)

              gTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTtggtg                                                                                                                                    <  1:1296508/150‑1 (MQ=255)

                 aTTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGgtggtg                                                                                                                                 >  2:2897453/1‑150 (MQ=255)

                  ttAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGgtgt                                                                                                                                >  1:7012312/1‑150 (MQ=255)

                    aaCGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTc                                                                                                                              <  2:1754584/150‑1 (MQ=255)

                    aaCGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTc                                                                                                                              >  1:6431377/1‑150 (MQ=255)

                     aCGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCa                                                                                                                             >  1:2001462/1‑150 (MQ=255)

                     aCGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCa                                                                                                                             >  2:2692372/1‑150 (MQ=255)

                     aCGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCa                                                                                                                             >  1:5833772/1‑150 (MQ=255)

                      cGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCaa                                                                                                                            <  1:1742799/150‑1 (MQ=255)

                      cGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCaa                                                                                                                            <  1:3982767/150‑1 (MQ=255)

                       ggCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAg                                                                                                                           <  2:1944601/150‑1 (MQ=255)

                         cAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCa                                                                                                                         <  1:2370810/150‑1 (MQ=255)

                         cAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCa                                                                                                                         <  2:4594633/150‑1 (MQ=255)

                            ggCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGtt                                                                                                                                        <  2:6906433/132‑1 (MQ=255)

                             gCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGaac                                                                                                                     >  2:8555658/1‑150 (MQ=255)

                                      cAGTTCATTGGCTACTGGGCCAAGAGCGAATGGACCATGGCGAAGAAAACTTCTGGCAAGGATTACCAGTGCGTGACGTTCCCCGGTACTGACAAGTCATTCATGTAAAGCATCTACTCGTTTGTGGTGTTCAAGCAGAACAACGCTTGc                                                                                                            >  2:5186523/1‑150 (MQ=255)

                                         aTCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCAcc                                                                                                         >  1:5724150/1‑150 (MQ=255)

                                              gggCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgc                                                                                                    >  2:4265384/1‑150 (MQ=255)

                                               ggCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGaacaac                                                                                                                  >  1:10021126/1‑135 (MQ=255)

                                               ggCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgct                                                                                                   <  1:7931673/150‑1 (MQ=255)

                                               ggCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgct                                                                                                   <  1:10209303/150‑1 (MQ=255)

                                               ggCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgct                                                                                                   <  1:6810230/150‑1 (MQ=255)

                                                gCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgctg                                                                                                  <  1:1434299/150‑1 (MQ=255)

                                                     tGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTcag                                                                                             >  2:9468501/1‑150 (MQ=255)

                                                       ggCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTcagca                                                                                           >  1:7819372/1‑150 (MQ=255)

                                                       ggCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTcagca                                                                                           <  2:1520008/150‑1 (MQ=255)

                                                        gCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTcagcag                                                                                          <  1:9470143/150‑1 (MQ=255)

                                                           aaGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGAc                                                                                       >  2:5439982/1‑150 (MQ=255)

                                                                       aCCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGTTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAg                                                                           >  2:3920357/1‑150 (MQ=255)

                                                                       aCCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAg                                                                           >  2:7158722/1‑150 (MQ=255)

                                                                         ccTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgctg                                                                                                  <  2:4772061/125‑1 (MQ=255)

                                                                            ggCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGtt                                                                      <  1:4176636/150‑1 (MQ=255)

                                                                               gaagaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTggg                                                                   <  1:999182/150‑1 (MQ=255)

                                                                               gaagaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACATCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTggg                                                                   >  2:5790694/1‑150 (MQ=255)

                                                                               gaagaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGGTGGTGGTGTTCACGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTggg                                                                   >  2:9027954/1‑150 (MQ=255)

                                                                                  gaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGa                                                                <  2:2403119/150‑1 (MQ=255)

                                                                                   aaaaCTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAg                                                               <  1:4932219/150‑1 (MQ=255)

                                                                                    aaaCTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAgg                                                              <  1:4906312/150‑1 (MQ=255)

                                                                                        tgctgGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGa                                                                >  2:5301799/1‑144 (MQ=255)

                                                                                          ctgGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTcc                                                        <  2:7509617/150‑1 (MQ=255)

                                                                                          ctgGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTcc                                                        <  2:9671159/150‑1 (MQ=255)

                                                                                             gCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAgg                                                                                         <  1:7106900/114‑1 (MQ=255)

                                                                                             gCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGa                                                     >  2:5668344/1‑150 (MQ=255)

                                                                                               aaGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTtggtg                                                                                                                                    <  2:9835514/69‑1 (MQ=255)

                                                                                               aaGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTc                                                         <  2:7073900/144‑1 (MQ=255)

                                                                                                aGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAgg                                                  <  1:8256310/150‑1 (MQ=255)

                                                                                                      aCCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCa                                            <  2:8202286/150‑1 (MQ=255)

                                                                                                       ccAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTca                                                                                              >  1:552936/1‑99 (MQ=255)

                                                                                                       ccAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAg                                                                           <  1:8473413/118‑1 (MQ=255)

                                                                                                       ccAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAg                                           >  2:7999915/1‑150 (MQ=255)

                                                                                                        cAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGACcagcagaggt                                                                                      >  1:8695358/1‑102 (MQ=255)

                                                                                                        cAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGACcagcagaggt                                                                                      <  2:8695358/106‑5 (MQ=255)

                                                                                                          gtgcgtgcCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCAt                                        >  2:1339695/1‑150 (MQ=255)

                                                                                                                gcCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATcaacaa                                  >  2:3709397/1‑150 (MQ=255)

                                                                                                                gcCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATcaacaa                                  <  1:3162074/150‑1 (MQ=255)

                                                                                                                gcCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATcaaaca                                  >  1:9401357/1‑147 (MQ=255)

                                                                                                                  cGTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAgg                                <  1:836689/150‑1 (MQ=255)

                                                                                                                   gTTCCCCGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAggg                               >  1:7390837/1‑150 (MQ=255)

                                                                                                                        ccGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGa                          >  2:4411992/1‑150 (MQ=255)

                                                                                                                        ccGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGa                          >  1:2990412/1‑150 (MQ=255)

                                                                                                                        ccGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGa                          <  2:3285374/150‑1 (MQ=255)

                                                                                                                        ccGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGa                          >  1:7576335/1‑150 (MQ=255)

                                                                                                                        ccGCTACAGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGAACATCAGCTGTTAAGCAGGACAACGCCCGCAAGGTCTTGGGTGAGGACTTCCAGAAGGACTTCAGCAACAACAAGGGTTCGa                          >  1:9316177/1‑150 (MQ=255)

                                                                                                                         cGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGaaca                                                                                                                    >  2:1056186/1‑59 (MQ=255)

                                                                                                                         cGGTACTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGaaca                                                                                                                    <  1:1056186/59‑1 (MQ=255)

                                                                                                                             aCTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCg                     <  1:2845441/150‑1 (MQ=255)

                                                                                                                             aCTGACAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCg                     <  1:2659139/150‑1 (MQ=255)

                                                                                                                             aCTGACAAGTCATTCCTGTACAGCATCGACTCGTTGGTGGTGATCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTAGATCCCg                     <  2:6622705/150‑1 (MQ=255)

                                                                                                                                 aCAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTcagca                                                                                           >  2:9215392/1‑76 (MQ=255)

                                                                                                                                 aCAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTcagca                                                                                           <  1:9215392/76‑1 (MQ=255)

                                                                                                                                 aCAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGaa                                                    >  2:8302626/1‑115 (MQ=255)

                                                                                                                                 aCAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAg                                                   <  2:9065492/116‑1 (MQ=255)

                                                                                                                                 aCAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAg                                                   >  1:9065492/1‑116 (MQ=255)

                                                                                                                                 aCAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATcc                       >  1:8766717/1‑144 (MQ=255)

                                                                                                                                 aCAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTAc                 >  2:142276/1‑150 (MQ=255)

                                                                                                                                 aCAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTAc                 <  1:3520177/150‑1 (MQ=255)

                                                                                                                                 aCAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTAc                 >  1:5393679/1‑150 (MQ=255)

                                                                                                                                 aCAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTAc                 >  1:9021091/1‑150 (MQ=255)

                                                                                                                                 aCAAGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTAGATCCCGGTAc                 <  2:3158972/150‑1 (MQ=255)

                                                                                                                                   aaGTCCTTCCTGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGc               >  1:968038/1‑150 (MQ=255)

                                                                                                                                      tccttcctGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAAc            >  2:2150592/1‑150 (MQ=255)

                                                                                                                                        cttcctGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGaa                                                    >  2:8744071/1‑108 (MQ=255)

                                                                                                                                        cttcctGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGa          <  2:7859/150‑1 (MQ=255)

                                                                                                                                        cttcctGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGa          >  1:4435739/1‑150 (MQ=255)

                                                                                                                                            ctGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGACCGCAAGGTGTTGGGTGAGGAATTCCAGAAGGTCTTCAGAATCAACAAGGGTTCGATCCCTGTACGCAACGACATg      >  2:7741728/1‑150 (MQ=255)

                                                                                                                                             tGTACAGCATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGc     >  1:6111862/1‑150 (MQ=255)

                                                                                                                                              tcaccacaTCGACTCGGTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCt    >  2:7009598/6‑150 (MQ=255)

TGGAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTG  >  NC_002947/1163586‑1163879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`