BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,146,499:1	+A	6.2%	coding (424/972 nt)	PP_2757 →	sugar‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,146,499	1	.	A	6.2%	1389.8 / 76.6	324	coding (424/972 nt)	PP_2757	sugar‑binding protein
Reads supporting (aligned to +/- strand): ref base . (160/144); new base A (9/10); total (169/155)
Fisher's exact test for biased strand distribution p-value = 8.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

ATGCGGTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCG  >  NC_002947/3146350‑3146648

aTGCGGTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCcgcg                                                                                                                                                        <  2:4415818/150‑1 (MQ=255)

 tGCGGTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCcgcg‑c                                                                                                                                                      <  1:6992884/150‑1 (MQ=255)

   cGGTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGTAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACG‑CGCGGCCGGCcgcgacgt                                                                                                                                                    <  2:1570455/150‑5 (MQ=255)

    ggTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGtt                                                                                                                                                   <  2:375858/150‑1 (MQ=255)

    ggTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACATTTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGtt                                                                                                                                                   <  1:2976304/150‑1 (MQ=255)

     gTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCGTCAACAACACCACCTCGGACACCGTCGCGGCCGGCCGCG‑CGTTg                                                                                                                                                  >  2:4493062/1‑150 (MQ=255)

     gTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACCCCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTg                                                                                                                                                  <  1:5804608/150‑1 (MQ=255)

      ttaTTCAGACCCTGGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGTTGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTgg                                                                                                                                                 <  1:2039166/148‑1 (MQ=255)

       aaTTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGc                                                                                                                                                <  1:8676639/150‑1 (MQ=255)

        aTTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGcc                                                                                                                                               <  1:5476191/150‑1 (MQ=255)

          tCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGCCAACG‑CGCGGCCGGCCGCGACGTTGTCCGa                                                                                                                                             <  2:6675285/150‑1 (MQ=255)

          tCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGa                                                                                                                                             >  1:8408072/1‑150 (MQ=255)

          tCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGGTGGGCGa                                                                                                                                             >  1:914123/1‑150 (MQ=255)

           cAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTGCAGCCTCAACAACACCACCTCGGACAACG‑CGCGGCCGGCCGCGACGTTGTCCGag                                                                                                                                            <  2:1786962/150‑2 (MQ=255)

           cAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGaa                                                                                                                                            >  2:3116879/1‑150 (MQ=255)

           cAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGGTGGCCGac                                                                                                                                            >  1:5618179/1‑149 (MQ=255)

             gACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGTTATCCCGCTTTTCACCATTTATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACa                                                                                                                                          <  1:4377970/150‑1 (MQ=255)

              aCCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCCCCTCGGACAACG‑CGCGGCCGGCCACGACGTTGTCCGaggtg                                                                                                                                         <  1:328730/150‑5 (MQ=255)

              aCCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGGCGAACAg                                                                                                                                         >  1:798662/1‑150 (MQ=255)

               cccACGGTCCCCTGAGCCTGATCGACCCCTGGCTCAACCGCATCCAGGCAGCAGGTATCCCGCTTTCCACCATTGAGGTGCCATCGCAGTACAGCCTCACCACCACCACCTCGGACAACGTCGCGGCCGGCCGCGCCGTTGTCCGaggtg                                                                                                                                         <  2:760170/150‑5 (MQ=255)

                ccTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCt                                                                                                                                       >  2:6147258/1‑150 (MQ=255)

                 cTCGTTACCGTGTGCGTGATCGACCCCTGGCTCAAGCGCATCCTGGCAGCAGGTATCCCGATTTTCACCATTTTTGTGCAATCGCAGTACAGCCTCAACAACCCCACCTGGGACACCGTGGCGGCGGGCCGCG‑CGTTGGCCGAACAGCTg                                                                                                                                      <  1:7669527/150‑1 (MQ=255)

                 cTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTg                                                                                                                                      <  2:4930525/150‑1 (MQ=255)

                   cGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACACCACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGt                                                                                                                                    <  2:7888398/150‑1 (MQ=255)

                       aCCCTGAGCGTTATCGACCCCTGGCTCAAGCGCATCCAGGCAGCTGGTAACCCGCTTTTCACCATTGATGTGCCATCGCAGAACAGCCTCAACAACCCCACCTCGCACAAGGTGGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTgcgc                                                                                                                                <  1:671640/150‑1 (MQ=255)

                           tGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGaca                                                                                                                            >  1:4563145/1‑150 (MQ=255)

                                  gATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGGTGGCCGAACACCGGGTGCGCGCCCCccgcgg                                                                                                                     >  1:857545/1‑150 (MQ=255)

                                   aTCGACCCCTGGCTAAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACAATTGTTGTGCCATCGCAGTACAGCCTCAACAACCCCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCggcggc                                                                                                                    <  1:8756828/150‑1 (MQ=255)

                                     cGACCCCTGGCTCACGCGCATCCAGGCAGCCTGTATCCCGCTTTTCACCTTGGATGTGCCATCGCAGTACAGCCTCAACAACACCCCCTCGGACACCGTCGCGGCGGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCaa                                                                                                                  <  1:7125598/150‑1 (MQ=255)

                                          ccTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACAACGGCGGCAAGGGcc                                                                                                             >  1:5385449/1‑150 (MQ=255)

                                           cTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCg                                                                                                            >  2:1367938/1‑150 (MQ=255)

                                                tCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATcc                                                                                                       >  1:1336587/1‑150 (MQ=255)

                                                    gcgcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑TGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGgt                                                                                                   >  2:3077922/1‑150 (MQ=255)

                                                    gcgcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTgga                                                                                                   >  2:7787040/1‑149 (MQ=255)

                                                        aTCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCAGGTGCGCGACACCGGCGGCCAGGGCCGCATCCGGGTGTTc                                                                                               >  1:8235143/1‑150 (MQ=255)

                                                        aTCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGACGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTc                                                                                               >  1:1926247/1‑150 (MQ=255)

                                                           ccggCAGCTGTTACCCCGTTTTTCACCTTTTTTGGGCCATCGCATTACCGCATCACCAACCCCACCCCGGACACCGGCGCGGCCGGCCGCG‑CGTGGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAAc                                                                                            <  1:209904/148‑1 (MQ=255)

                                                              gcagcaGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGc                                                                                         <  2:1689285/150‑1 (MQ=255)

                                                                  cagGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCTGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCt                                                                                     <  2:6429156/150‑1 (MQ=255)

                                                                   agGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACCGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGACCAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGGGTGCACCGGCTTCtc                                                                                    >  2:7411975/1‑149 (MQ=255)

                                                                   agGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCACCAACACCCCCTCGGACACCGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTa                                                                                    <  2:202945/150‑1 (MQ=255)

                                                                   agGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTa                                                                                    >  2:1035743/1‑150 (MQ=255)

                                                                      tATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACgg                                                                                 <  2:353098/150‑1 (MQ=255)

                                                                      tATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGGTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCGTCTACgg                                                                                 >  1:7833933/1‑150 (MQ=255)

                                                                       aTCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACggg                                                                                <  2:7068530/150‑1 (MQ=255)

                                                                        tCCCGCTTTTCACCATTTTTGTCCCACCGCAGAACAGCCAAAACCACCCCACCGCGGCCACCGCCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACgggg                                                                               <  1:1004279/150‑1 (MQ=255)

                                                                          ccGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTg                                                                             >  2:2405565/1‑150 (MQ=255)

                                                                           cGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCCCCTCGGACAACGTCGCGGCCGGCCGCG‑CGTGGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGc                                                                            <  2:8169327/150‑1 (MQ=255)

                                                                           cGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGc                                                                            >  1:1155680/1‑150 (MQ=255)

                                                                            gCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGcc                                                                           >  2:3005548/1‑150 (MQ=255)

                                                                                ttCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTc                                                                       >  2:5860706/1‑150 (MQ=255)

                                                                                  cACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtg                                                                     >  2:3891519/1‑150 (MQ=255)

                                                                                   aCCTTTGTTGTGCCACCGCATAACAGCCACAACAACCCCCCCCCGGACACCGGCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgt                                                                    <  1:1811585/150‑1 (MQ=255)

                                                                                   aCCATTGCTGTGCCATCGCAGTACAGCCTCAACACCACCCCCGCGCACACCGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgt                                                                    <  1:4719824/150‑1 (MQ=255)

                                                                                   aCCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGTAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgt                                                                    >  2:8519647/1‑150 (MQ=255)

                                                                                   aCCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgt                                                                    <  2:2753386/150‑1 (MQ=255)

                                                                                    ccttggATGTGCCATCGCATTACAGCCTCAACACCACCACCTCGCACAACGGCGCGGCCGGCCGCG‑CGTGGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgtg                                                                   <  1:5967412/145‑1 (MQ=255)

                                                                                     cATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCAGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGGGCCGGTCTGTGc                                                                  >  2:6206772/1‑150 (MQ=255)

                                                                                      aTTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCg                                                                 >  1:2220677/1‑150 (MQ=255)

                                                                                      aTTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCg                                                                 >  2:1542103/1‑150 (MQ=255)

                                                                                      aTTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCGGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCg                                                                 >  1:4489721/1‑150 (MQ=255)

                                                                                      aTTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGGTGGCCGAACAGCTGGTGCGCGACACCGGCGGCCAGGGCCGCCTCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCGGTGCg                                                                 >  1:5930333/1‑150 (MQ=255)

                                                                                         gttgtgCCATCGCAGTACAGCATCAACAACACCACCTCGTACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATc                                                              <  1:7067891/148‑1 (MQ=255)

                                                                                          aTGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATcc                                                             >  2:4630933/1‑150 (MQ=255)

                                                                                           tgtgCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCg                                                            <  2:8263661/150‑1 (MQ=255)

                                                                                               ccATCGCATAACCCCCACAACACCACCACCGCGCACAACGGCGCGGCCGCCCGCG‑CGTGGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTac                                                        <  1:5143891/150‑1 (MQ=255)

                                                                                               ccATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTac                                                        >  2:4840333/1‑150 (MQ=255)

                                                                                                cATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTacg                                                       >  2:6722758/1‑150 (MQ=255)

                                                                                                 aTCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTacga                                                      >  1:2804296/1‑150 (MQ=255)

                                                                                                 aTCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTacga                                                      >  2:3630586/1‑150 (MQ=255)

                                                                                                 aTCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTacga                                                      >  1:6440005/1‑150 (MQ=255)

                                                                                                 aTCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGGTGGCCGAACAGCTGGTGCGCGACACCGGCGGCCAGGGCCGCCTCCGGGGGTTCAACGGCGTCGACGGGGTGCCGGTCTGTGCGAGCCGcgacga                                                      >  1:2201097/1‑150 (MQ=255)

                                                                                                    gCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCGGGTGTTCAACGGCTTCTACGGGGGGCCGGTCTGTGCGATCCGCTacgacgg                                                   >  1:6359916/1‑149 (MQ=255)

                                                                                                     cAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGACCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAg                                                  <  1:6812574/150‑1 (MQ=255)

                                                                                                         aCAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTga                                              >  2:2875178/1‑150 (MQ=255)

                                                                                                            gCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTgaaga                                           <  2:4275533/150‑1 (MQ=255)

                                                                                                             ccTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTgaagaa                                          >  2:321854/1‑150 (MQ=255)

                                                                                                             caacaacacCCCCCCCTCGCACAACGCCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTgaagaa                                          <  1:160838/147‑1 (MQ=255)

                                                                                                               tCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAgg                                        >  1:3427635/1‑150 (MQ=255)

                                                                                                                     acacCACCACGCACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTgg                                  <  2:914123/150‑1 (MQ=255)

                                                                                                                      caccacCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGc                                 <  2:5269770/150‑1 (MQ=255)

                                                                                                                       accaccTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGcc                                <  2:6223091/150‑1 (MQ=255)

                                                                                                                         ctgttCGGCCAACG‑CGCGGCCGGCCGCGACGTTGTCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGa                              >  2:5049247/5‑150 (MQ=38)

                                                                                                                          cccTCGGACACCGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGat                             <  2:5418771/149‑1 (MQ=255)

                                                                                                                          accTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGat                             <  2:8417751/150‑1 (MQ=255)

                                                                                                                               ggACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatcatac                        >  1:7406534/1‑148 (MQ=255)

                                                                                                                               ggACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATcc                        >  2:5601748/1‑150 (MQ=255)

                                                                                                                                gACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCg                       >  1:2420678/1‑150 (MQ=255)

                                                                                                                                gACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCg                       >  2:6366319/1‑150 (MQ=255)

                                                                                                                                 aCAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACTGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGc                      <  2:4689913/150‑1 (MQ=255)

                                                                                                                                  ccaacgcgcgGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCa                     >  1:1471032/7‑150 (MQ=255)

                                                                                                                                   aaCGTCGCGGCCGGCCGCG‑CGTTGGCCGAACGGCTGGTGCGCGACCCCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCGTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCaa                    >  1:4963231/1‑150 (MQ=255)

                                                                                                                                   aaCGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCaa                    >  2:6563119/1‑150 (MQ=255)

                                                                                                                                   aaCGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCaa                    <  2:5789722/150‑1 (MQ=255)

                                                                                                                                   aaCGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCaa                    <  2:2154889/150‑1 (MQ=255)

                                                                                                                                       gcgcgGCCGGCCGCGACGTTGTCCG‑‑‑AGGTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAg              >  2:3468995/2‑150 (MQ=255)

                                                                                                                                          cggccggcCGCG‑CGTGGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGGTGAAGAAGGTACTGGCCGATCATCCGCACTTGCAGa             >  2:7019504/1‑150 (MQ=255)

                                                                                                                                                 ccgCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCt                                                                      >  2:5708917/1‑86 (MQ=255)

                                                                                                                                                 ccgCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCt                                                                      <  1:5708917/86‑1 (MQ=255)

                                                                                                                                                     g‑cgTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCg  >  1:3957422/1‑150 (MQ=255)

ATGCGGTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCG  >  NC_002947/3146350‑3146648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`