BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	255,257	C→T	5.9%	intergenic (‑141/+22)	PP_0203 ← / ← PP_t74	dipeptidase/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	255,257	0	C	T	5.9%	1077.7 / 35.6	340	intergenic (‑141/+22)	PP_0203/PP_t74	dipeptidase/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base C (173/146); new base T (10/10); total (184/156)
Fisher's exact test for biased strand distribution p-value = 8.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 342 total aligned reads displayed.

CAACTGCATGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGAT  >  NC_002947/255108‑255405

 aaCTGCATGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCa                                                                                                                                                     <  2:6212172/150‑1 (MQ=255)

      cATGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGccc                                                                                                                                                <  2:8028174/150‑1 (MQ=255)

          gTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAggg                                                                                                                                            <  2:2621680/150‑1 (MQ=255)

           ttGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGc                                                                                                                                           >  1:3068924/1‑150 (MQ=255)

                cgcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTAt                                                                                                                                      >  1:8271085/1‑150 (MQ=255)

                cgcAGTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCTGGGCCTGAGCCCGGGGCttttt                                                                                                                                      <  1:6019009/150‑3 (MQ=255)

                 gcaggccTTTGCCAATCATTGTTCAGGGTTACCCGGTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTAtt                                                                                                                                     <  2:7678027/145‑1 (MQ=255)

                 gcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTAtt                                                                                                                                     <  2:3710968/150‑1 (MQ=255)

                     tCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGata                                                                                                                                 <  1:1972495/150‑1 (MQ=255)

                       cTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATg                                                                                                                               >  1:795591/1‑150 (MQ=255)

                             cAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCAc                                                                                                                         >  2:4366591/1‑150 (MQ=255)

                              aaTCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACt                                                                                                                        >  1:4757210/1‑150 (MQ=255)

                              aaTCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACt                                                                                                                        >  2:166840/1‑150 (MQ=255)

                                  aTTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTcggc                                                                                                                    >  1:447785/1‑150 (MQ=255)

                                  aTTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTcggc                                                                                                                    >  1:6755079/1‑150 (MQ=255)

                                   ttGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTcggcg                                                                                                                   <  2:2073036/150‑1 (MQ=255)

                                     gTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCggg                                                                                                                 >  1:36775/1‑150 (MQ=255)

                                      ttCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGa                                                                                                                >  2:1168116/1‑150 (MQ=255)

                                            gTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGaa                                                                                                          >  1:6454239/1‑150 (MQ=255)

                                              tACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAAcc                                                                                                        <  2:8088273/150‑1 (MQ=255)

                                                cccGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCa                                                                                                      <  1:1897090/150‑1 (MQ=255)

                                                    ttCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGAc                                                                                                  >  1:1439672/1‑150 (MQ=255)

                                                     tCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGAcc                                                                                                 <  1:1313000/150‑1 (MQ=255)

                                                        ggAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCt                                                                                              >  2:4031796/1‑150 (MQ=255)

                                                        ggAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCt                                                                                              <  1:471143/150‑1 (MQ=255)

                                                         gAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTg                                                                                             >  1:955084/1‑150 (MQ=255)

                                                          aaaCGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTGCGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGc                                                                                            <  2:4266253/150‑1 (MQ=255)

                                                              gggAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTc                                                                                        >  1:7859681/1‑150 (MQ=255)

                                                               ggAAGACGCCCATGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCg                                                                                       <  1:8396000/150‑1 (MQ=255)

                                                                 aaGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGa                                                                                     <  2:330590/150‑1 (MQ=255)

                                                                   gACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAgg                                                                                   <  2:8506455/150‑1 (MQ=255)

                                                                   gACGCCCACGCTAACACCAATGACCCGTGGTAAGGGAGTACCAGAAAGGAGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAgg                                                                                   >  1:2185540/1‑150 (MQ=255)

                                                                    aCGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAggg                                                                                  <  2:5113411/150‑1 (MQ=255)

                                                                      gCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCa                                                                                >  1:2461164/1‑150 (MQ=255)

                                                                        ccACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGTGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGt                                                                              >  1:7342148/1‑150 (MQ=255)

                                                                        ccACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCCGt                                                                              >  1:535674/1‑150 (MQ=255)

                                                                        ccACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGt                                                                              >  1:8136275/1‑150 (MQ=255)

                                                                        ccACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGt                                                                              <  1:8223345/150‑1 (MQ=255)

                                                                        ccACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGt                                                                              <  1:1158036/150‑1 (MQ=255)

                                                                          acacTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTAc                                                                            >  1:4975345/4‑150 (MQ=255)

                                                                              tAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTa                                                                        <  1:3791777/150‑1 (MQ=255)

                                                                              tAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTa                                                                        >  2:7824227/1‑150 (MQ=255)

                                                                              tAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTa                                                                        >  1:2745470/1‑150 (MQ=255)

                                                                              tAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTa                                                                        >  2:2728/1‑150 (MQ=255)

                                                                              tAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTa                                                                        <  2:2551341/150‑1 (MQ=255)

                                                                              tAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTa                                                                        <  1:5437975/150‑1 (MQ=255)

                                                                              tAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCCTCCGAAGGCAggggtcgt                                                                        <  1:2406379/150‑8 (MQ=255)

                                                                                acacCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATc                                                                      <  2:1582620/150‑1 (MQ=255)

                                                                                 cacCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATcc                                                                     <  2:1532701/150‑1 (MQ=255)

                                                                                  acCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCCGTACTCTATCCa                                                                    >  1:4353422/1‑150 (MQ=255)

                                                                                  acCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCa                                                                    >  1:890898/1‑150 (MQ=255)

                                                                                    cAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGc                                                                  >  1:4896763/1‑150 (MQ=255)

                                                                                     aaTGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCt                                                                 >  1:123002/1‑150 (MQ=255)

                                                                                       tGACCCGTGGTACGGGAGTACCAGAACGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATAGGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGa                                                               <  2:8175405/150‑1 (MQ=255)

                                                                                       tGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGa                                                               <  1:417755/150‑1 (MQ=255)

                                                                                       tGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGa                                                               <  1:6161908/150‑1 (MQ=255)

                                                                                          cccGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCt                                                            >  1:341710/1‑150 (MQ=255)

                                                                                          cccGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCt                                                            >  2:3512500/1‑150 (MQ=255)

                                                                                                   cGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCa                                                   <  2:3762331/150‑1 (MQ=255)

                                                                                                    gggAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCaa                                                  <  2:5754896/150‑1 (MQ=255)

                                                                                                        gTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAAcgcg                                              >  2:3126061/1‑150 (MQ=255)

                                                                                                          aCCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgca                                            >  1:1649872/1‑150 (MQ=255)

                                                                                                           ccAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcac                                           <  1:3218869/150‑1 (MQ=255)

                                                                                                            cAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCa                                                                                                      >  2:6270248/1‑90 (MQ=255)

                                                                                                            cAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcacg                                          >  1:3851342/1‑150 (MQ=255)

                                                                                                             aGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcacgc                                         >  1:720536/1‑150 (MQ=255)

                                                                                                             aGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcacgc                                         >  1:2777192/1‑150 (MQ=255)

                                                                                                              gAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcacgca                                        >  2:7536478/1‑150 (MQ=255)

                                                                                                               aaaGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCACCGCGCACGCAt                                       >  2:6979799/1‑150 (MQ=255)

                                                                                                               aaaGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCAt                                       <  2:7135883/150‑1 (MQ=255)

                                                                                                               aaaGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCAt                                       >  2:6591298/1‑150 (MQ=255)

                                                                                                                 aGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGa                                     <  2:5450584/150‑1 (MQ=255)

                                                                                                                    tGACATGTCGCACAATCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGTCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATAc                                  <  2:3243638/150‑1 (MQ=255)

                                                                                                                    tGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATAc                                  <  1:698109/150‑1 (MQ=255)

                                                                                                                     gACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATAcc                                 >  2:6394552/1‑150 (MQ=255)

                                                                                                                      aCATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATAccc                                <  2:6729159/150‑1 (MQ=255)

                                                                                                                        aTGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGAccc                                                                                                >  2:4910916/1‑84 (MQ=255)

                                                                                                                          gTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCt                            <  2:1862996/150‑1 (MQ=255)

                                                                                                                           tCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCtg                           >  2:397626/1‑150 (MQ=255)

                                                                                                                           tCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCtg                           >  1:3980907/1‑150 (MQ=255)

                                                                                                                           tCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCtg                           >  1:4226062/1‑150 (MQ=255)

                                                                                                                            cGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCtgt                          <  2:3590704/150‑1 (MQ=255)

                                                                                                                            cGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCtgt                          <  1:4399619/150‑1 (MQ=255)

                                                                                                                                    cGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTTCAACGCGCACGCATGATACCCATCTGTGTCGTTGa                  <  1:5927769/150‑1 (MQ=255)

                                                                                                                                     gAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGAc                 >  2:5903482/1‑150 (MQ=255)

                                                                                                                                     gAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGAc                 >  1:6844552/1‑150 (MQ=255)

                                                                                                                                     aataaaGCCCTGGGCCGGAGCCCGGGGCTTTTTTGATATGGCGCACGCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGAc                 >  2:4619980/4‑150 (MQ=255)

                                                                                                                                       taaaGCCCTGGGCCTGAGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGt               >  1:7787937/2‑150 (MQ=255)

                                                                                                                                       aaaaGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGt               >  2:2366528/1‑150 (MQ=255)

                                                                                                                                       aaaaGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGt               <  1:2252735/150‑1 (MQ=255)

                                                                                                                                         aaGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGaa                                                                                                          >  1:2615440/1‑57 (MQ=255)

                                                                                                                                         aaGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTcc             >  1:4434163/1‑150 (MQ=255)

                                                                                                                                          aGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCt                                                                                              <  2:2821575/68‑1 (MQ=255)

                                                                                                                                            ccccGGGCTCAGGCCGAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGGCTTCGGAGGGCTGTACGCTATCCAGCTGAGCTACGAGTGCAACGCGCTCGCATGATACCCATCTGTGTCGTTGACGTCCATc          <  2:4221757/150‑1 (MQ=255)

                                                                                                                                              ccGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGc        >  1:5196981/1‑150 (MQ=255)

                                                                                                                                                gggCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCt      >  2:4719005/1‑150 (MQ=255)

                                                                                                                                                 ggCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCtt     <  1:4091029/150‑1 (MQ=255)

                                                                                                                                                    tCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGAt  >  2:1961157/1‑150 (MQ=255)

                                                                                                                                                    tCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGAt  >  1:7494170/1‑150 (MQ=255)

                                                                                                                                                    ccAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGAt  <  1:1046011/149‑1 (MQ=255)

CAACTGCATGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGAT  >  NC_002947/255108‑255405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`