BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,781,102	G→C	5.0%	A153P (GCG→CCG) ‡	sotB →	sugar efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,781,102	0	G	C	5.0%	1048.6 / 30.7	312	A153P (GCG→CCG) ‡	sotB	sugar efflux transporter
Reads supporting (aligned to +/- strand): ref base G (145/150); new base C (6/10); total (152/160)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 336 total aligned reads displayed.

TCATTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATC  >  NC_002947/2780953‑2781250

 cATTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGgc                                                                                                                                                     <  1:4985599/150‑1 (MQ=255)

  aTTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGgcg                                                                                                                                                    >  2:3255597/1‑150 (MQ=255)

  aTTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGgcg                                                                                                                                                    >  1:875912/1‑150 (MQ=255)

    tGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCt                                                                                                                                                  <  2:7071066/150‑1 (MQ=255)

     gTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTg                                                                                                                                                 <  1:4090814/150‑1 (MQ=255)

     gTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCAGTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTg                                                                                                                                                 <  2:2025247/150‑1 (MQ=255)

      tCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTgg                                                                                                                                                <  2:6658743/150‑1 (MQ=255)

       cAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTggg                                                                                                                                               <  1:7343784/150‑1 (MQ=255)

          ccACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCct                                                                                                                                            <  2:8766423/150‑1 (MQ=255)

           cACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCctg                                                                                                                                           <  1:3503741/150‑1 (MQ=255)

             cTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCCAGCGCCTTGGCctgct                                                                                                                                         <  2:1340181/150‑1 (MQ=255)

             cTTGCCGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCctgct                                                                                                                                         <  2:7768819/150‑1 (MQ=255)

              ttGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCctgctg                                                                                                                                        <  1:2779624/150‑1 (MQ=255)

              ttGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCctgctg                                                                                                                                        <  1:6776053/150‑1 (MQ=255)

              ttGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCGGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCCAGCGCCTTGGCctgctg                                                                                                                                        <  2:8168271/150‑1 (MQ=255)

                    tCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCcccc                                                                                                                                  >  1:1425054/1‑150 (MQ=255)

                    tCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCAcc                                                                                                                                  >  1:6351901/1‑150 (MQ=255)

                       tGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGt                                                                                                                               >  2:4842185/1‑150 (MQ=255)

                       tGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGt                                                                                                                               >  2:8474114/1‑150 (MQ=255)

                        ggCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGta                                                                                                                              <  1:5968837/150‑1 (MQ=255)

                           tttCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCCGCCGGCCAAGGCGCGGGGCCGGCTGGCCACCCGtccaa                                                                                                                           >  1:767630/1‑146 (MQ=255)

                           tttCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGtacaa                                                                                                                           >  1:6330791/1‑148 (MQ=255)

                           tttCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCGGCTGGCCACCGGtacta                                                                                                                           >  1:8159243/1‑150 (MQ=255)

                                 aaaGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTgg                                                                                                                     >  2:67273/1‑150 (MQ=255)

                                  aaGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGc                                                                                                                    <  2:7161964/150‑1 (MQ=255)

                                   aGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGcc                                                                                                                   >  2:4775379/1‑150 (MQ=255)

                                     cTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCAt                                                                                                                 <  1:151843/150‑1 (MQ=255)

                                      ttCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAACGCGCTGGGCCGGCTGGCCACCCGTACTACCCAGGCCaag                                                                                                                >  1:1044080/1‑148 (MQ=255)

                                         gCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTc                                                                                                             <  2:5423470/150‑1 (MQ=255)

                                           cATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCGGGGCCGGCTGGCCACCGGGACTACCCTGGCCATGGTCCt                                                                                                           >  1:494141/1‑150 (MQ=255)

                                            aTGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTg                                                                                                          <  1:2169355/150‑1 (MQ=255)

                                            aTGCGGCTGACCAGCAGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTg                                                                                                          >  2:2067507/1‑150 (MQ=255)

                                                  ctgGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCGGCTGGCCCCCGGTACTACCCTGGCCATGGTCCTGGGCCTc                                                                                                    >  2:1465458/1‑150 (MQ=255)

                                                     gTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCg                                                                                                 >  2:5552223/1‑150 (MQ=255)

                                                     gTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCCGTACTACCCTGGCCATGGTCCTgggggtgccg                                                                                                 >  1:4290436/1‑143 (MQ=255)

                                                      tCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCCGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCCGTACTACCCAGGCCAAGGTCCTGGGccaccccc                                                                                                >  1:6650907/1‑143 (MQ=255)

                                                       cAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCt                                                                                               <  2:4584713/150‑1 (MQ=255)

                                                        aGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCGGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTg                                                                                              >  1:7732207/1‑150 (MQ=255)

                                                         gCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTgg                                                                                             <  1:7987510/150‑1 (MQ=255)

                                                          ccGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCCAGCGCCTTGGCCTGCTgg                                                                                                                                       <  2:2887568/107‑1 (MQ=38)

                                                              aTCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCgt                                                                                        <  2:3360194/150‑1 (MQ=255)

                                                               tCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCCGTACTACCCTGGCCATGGTCCTGGGCAACCCGCGGGGCCgtg                                                                                       >  2:6283890/1‑150 (MQ=255)

                                                               tCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCGGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCgtg                                                                                       <  2:3811722/150‑1 (MQ=255)

                                                                 ggCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGtgtg                                                                                     <  2:5687416/150‑1 (MQ=255)

                                                                  gCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTgtgg                                                                                    >  2:437555/1‑150 (MQ=255)

                                                                   cATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTgtggt                                                                                   <  1:871489/150‑1 (MQ=255)

                                                                       gCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGc                                                                               <  2:5227891/150‑1 (MQ=255)

                                                                        cccTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCCAGCGCCTTGGCctgctg                                                                                                                                        <  2:2609593/92‑1 (MQ=37)

                                                                        cccTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCg                                                                              >  1:3566351/1‑150 (MQ=255)

                                                                         ccTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGa                                                                             <  1:1279765/150‑1 (MQ=255)

                                                                         ccTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGa                                                                             <  2:8610072/150‑1 (MQ=255)

                                                                         ccTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGa                                                                             <  1:5498143/150‑1 (MQ=255)

                                                                         ccTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAACGCGCTGGGCCGGCTGGCCACCGGTACAACCCAGGCCATGGTCCAGGGCATCCCGCTGGGCCGGGTGGTGGGCgc                                                                             >  1:6090356/1‑149 (MQ=255)

                                                                          cTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAg                                                                            <  2:6400103/150‑1 (MQ=255)

                                                                           tGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAgg                                                                           <  1:7018975/150‑1 (MQ=255)

                                                                               gcATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCACCCCGCCAAGGCGCAGGGCCGGCTGGCCACCCGGACTACCCAGGCCAGGGGCCGGGGCAACCCCCGGGGCCGGGTGGTGGGCGAGGCAcg                                                                       >  1:3866521/1‑149 (MQ=255)

                                                                                cATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTg                                                                      <  1:2581119/150‑1 (MQ=255)

                                                                                     ggTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTg                                                                 >  2:4449622/1‑150 (MQ=255)

                                                                                       tgtTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCCGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTgggcgggc                                                               <  1:5614460/150‑1 (MQ=255)

                                                                                       tgtTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGc                                                               <  2:2762350/150‑1 (MQ=255)

                                                                                       tgtTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGc                                                               <  1:3662168/150‑1 (MQ=255)

                                                                                            tGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCgt                                                                                        >  2:8695342/1‑120 (MQ=255)

                                                                                              gTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCAc                                                        >  2:4998798/1‑150 (MQ=255)

                                                                                                   tCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCGGGGCCGGCTGGCCACCGGTACTACCCGGGCCAAGGTCCGGGGCATCCCGCGGGGCCGGGTGGTGGGCGAGGCACTGGGCGGGCGGATCCCGGTcc                                                   >  1:8308213/1‑150 (MQ=255)

                                                                                                    cACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCt                                                  <  2:6793977/150‑1 (MQ=255)

                                                                                                     aCCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCGGCTGGCCACCGGTAATACCCAGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGGGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTg                                                 >  1:5730797/1‑150 (MQ=255)

                                                                                                      ccGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGa                                                <  2:3566357/150‑1 (MQ=255)

                                                                                                      ccGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGa                                                <  1:528818/150‑1 (MQ=255)

                                                                                                       cGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCGGGGCCGGCTGGCCACCGGTACGACCCTGGGCATGGTCCTGGGCCTCCCCCTGGGCCGTGTGGGGGGCGAGGCCCGGGGCTGGCGGTTCAAGTTCCagag                                               >  1:2632909/1‑150 (MQ=255)

                                                                                                             gCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGc                                         <  2:2975091/150‑1 (MQ=255)

                                                                                                              ccggCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGcc                                        >  1:5855620/3‑150 (MQ=255)

                                                                                                               tGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCg                                       <  2:6308772/150‑1 (MQ=255)

                                                                                                                ggCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCgg                                      <  1:3096081/150‑1 (MQ=255)

                                                                                                                ggCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCgg                                      <  2:244055/150‑1 (MQ=255)

                                                                                                                ggCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCgg                                      <  2:1105476/150‑1 (MQ=255)

                                                                                                                 gCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCAGGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATGGCCggg                                     <  2:6984481/150‑1 (MQ=255)

                                                                                                                    gTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTa                                  <  2:7278244/150‑1 (MQ=255)

                                                                                                                     tGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAg                                 <  1:3395328/150‑1 (MQ=255)

                                                                                                                      gCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAgc                                <  2:4221780/150‑1 (MQ=255)

                                                                                                                      gCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAgc                                <  1:1122297/150‑1 (MQ=255)

                                                                                                                         tgtgGCGCCGCCGGGCCAGCACGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGGGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCt                             >  2:4636758/1‑150 (MQ=255)

                                                                                                                          gtgGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTa                            <  2:6095994/150‑1 (MQ=255)

                                                                                                                          gtgGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCGGCTGGCCCCCGGTACTACCCAGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGGGGTGGGCGAGGCACTGGGCGGGCGTATCACGTTCCTGAGCCTTGCCGGGGTAGCGCTa                            >  2:2283285/1‑150 (MQ=255)

                                                                                                                             gcgcCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGcc                         <  1:8470194/150‑1 (MQ=255)

                                                                                                                               gccgccGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGccac                       <  2:8500308/150‑1 (MQ=255)

                                                                                                                                  cggtggCCAGCAGGCCCAGCGCCTTGGCCTGCTGGCCCCCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAg                                                                            >  1:6492017/5‑94 (MQ=21)

                                                                                                                                      ggCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCtt                <  1:1886214/150‑1 (MQ=255)

                                                                                                                                          agcagGCCCAGCGCCTTGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGcc            >  2:7651418/1‑150 (MQ=255)

                                                                                                                                          agcagGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGcc            >  1:6133933/1‑150 (MQ=255)

                                                                                                                                          agcagGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGcc            >  2:1627922/1‑150 (MQ=255)

                                                                                                                                          agcagGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGcc            >  2:3514197/1‑150 (MQ=255)

                                                                                                                                           gcagGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCt           >  1:2236081/1‑150 (MQ=255)

                                                                                                                                            cagGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTg          >  1:6617717/1‑150 (MQ=255)

                                                                                                                                             agGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGTGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGa         <  1:1955198/150‑1 (MQ=255)

                                                                                                                                               gCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTCCCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGGATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGTTc       <  2:6996701/150‑1 (MQ=255)

                                                                                                                                                 cAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCaa     >  2:3457019/1‑150 (MQ=255)

                                                                                                                                                 cAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCaa     >  1:5144480/1‑150 (MQ=255)

                                                                                                                                                  aaGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCaaa    >  2:1927165/1‑150 (MQ=255)

                                                                                                                                                    ggCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATc  >  1:8584009/1‑150 (MQ=255)

                                                                                                                                                    ggCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATc  <  1:4842185/150‑1 (MQ=255)

TCATTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATC  >  NC_002947/2780953‑2781250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`