BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,005,757	T→G	5.5%	V77V (GTA→GTC)	PP_2624 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,005,757	0	T	G	5.5%	1271.3 / 42.7	371	V77V (GTA→GTC)	PP_2624	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (136/214); new base G (12/9); total (148/223)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 376 total aligned reads displayed.

TTCAGGGCGACCAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCCACCTC  >  NC_002947/3005608‑3005906

ttCAGGGCGACCAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGt                                                                                                                                                       <  1:2706770/150‑1 (MQ=255)

     ggCGACCAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGt                                                                                                                                                  >  2:8409882/1‑150 (MQ=255)

           cAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTc                                                                                                                                            <  1:5925672/150‑1 (MQ=255)

           cAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTc                                                                                                                                            >  2:7397948/1‑150 (MQ=255)

           cAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTc                                                                                                                                            <  2:2213410/150‑1 (MQ=255)

             gTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCa                                                                                                                                          >  1:7544944/1‑150 (MQ=255)

             gTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCa                                                                                                                                          <  1:3079565/150‑1 (MQ=255)

              tGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAg                                                                                                                                         <  1:5132287/150‑1 (MQ=255)

                 ttCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGTACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTAGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCt                                                                                                                                      >  2:7595913/1‑150 (MQ=255)

                 ttCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCt                                                                                                                                      >  1:6094933/1‑150 (MQ=255)

                  tCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTc                                                                                                                                     <  1:7322157/150‑1 (MQ=255)

                  tCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTc                                                                                                                                     <  1:4410652/150‑1 (MQ=255)

                  tCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTc                                                                                                                                     <  2:8212980/150‑1 (MQ=255)

                    gcgcAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTcac                                                                                                                                   >  2:4749439/1‑150 (MQ=255)

                        aGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTg                                                                                                                               <  1:6238872/150‑1 (MQ=255)

                          cgcCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGc                                                                                                                             <  2:3211247/150‑1 (MQ=255)

                             cagcagCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGcgtc                                                                                                                          >  1:6037409/1‑150 (MQ=255)

                             cagcagCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGcgtc                                                                                                                          >  2:1046898/1‑150 (MQ=255)

                               gcagcTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGcgtcgt                                                                                                                        >  1:8072578/1‑150 (MQ=255)

                                cagcTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGtt                                                                                                                       <  2:3026329/150‑1 (MQ=255)

                                 agcTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTc                                                                                                                      <  2:3233009/150‑1 (MQ=255)

                                 agcTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTc                                                                                                                      <  1:7485250/150‑1 (MQ=255)

                                 agcTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTc                                                                                                                      <  1:1533316/150‑1 (MQ=255)

                                 agcTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTc                                                                                                                      <  1:5957578/150‑1 (MQ=255)

                                 agcTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTc                                                                                                                      <  1:577297/150‑1 (MQ=255)

                                        tCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGc                                                                                                               >  2:1175879/1‑150 (MQ=255)

                                            cTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGtt                                                                                                           <  2:7297460/150‑1 (MQ=255)

                                            cTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGtt                                                                                                           >  2:7851019/1‑150 (MQ=255)

                                            cTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGtt                                                                                                           <  2:2926190/150‑1 (MQ=255)

                                            cTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGtt                                                                                                           <  1:411067/150‑1 (MQ=255)

                                             tCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTg                                                                                                          <  1:8375394/150‑1 (MQ=255)

                                              cGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGa                                                                                                         <  2:7691891/150‑1 (MQ=255)

                                              cGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGa                                                                                                         <  2:5610922/150‑1 (MQ=255)

                                              cGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGa                                                                                                         <  2:2239868/150‑1 (MQ=255)

                                               gggCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACAGCGTGTTGaa                                                                                                        <  1:6596977/150‑1 (MQ=255)

                                               gggCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGaa                                                                                                        <  2:6830849/150‑1 (MQ=255)

                                               gggCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGaa                                                                                                        >  2:6350791/1‑150 (MQ=255)

                                                ggCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAat                                                                                                       <  2:8532669/150‑1 (MQ=255)

                                                ggCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCATTCAACAGCGTGTTGaac                                                                                                       <  1:1017690/150‑2 (MQ=255)

                                                   aCCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAatgta                                                                                                    >  2:7024695/1‑150 (MQ=255)

                                                   aCCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCATTCAACAGCGTGTTGaacgac                                                                                                    <  1:8409882/150‑5 (MQ=255)

                                                     cTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACAGCGTGTTGaacgacgc                                                                                                  <  1:2544560/150‑7 (MQ=255)

                                                     cTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAatgtatg                                                                                                  >  2:1802440/1‑150 (MQ=255)

                                                          gcgcgATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTAt                                                                                             <  1:426324/150‑1 (MQ=255)

                                                          gcgcgATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGGATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGGCGTTCAACACGCTGTTGAATGTCTGGTTAt                                                                                             >  2:4447199/1‑150 (MQ=255)

                                                            gcgATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCg                                                                                           <  2:2327147/150‑1 (MQ=255)

                                                              gATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGAt                                                                                         <  2:622292/150‑1 (MQ=255)

                                                              gATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGAt                                                                                         >  1:2853342/1‑150 (MQ=255)

                                                              gATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGAt                                                                                         <  2:6522356/150‑1 (MQ=255)

                                                               aTCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATg                                                                                        <  2:5064376/150‑1 (MQ=255)

                                                                tccggtccggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATgc                                                                                       >  2:264244/1‑150 (MQ=255)

                                                                tccggtccggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATgc                                                                                       <  1:7047910/150‑1 (MQ=255)

                                                                       cggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGAc                                                                                <  2:2769818/150‑1 (MQ=255)

                                                                        ggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGAcc                                                                               <  1:7289177/150‑1 (MQ=255)

                                                                         gCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCt                                                                              <  2:4842026/150‑1 (MQ=255)

                                                                           ccGAAATCGTTGATGGACCTGAACGGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGa                                                                            <  2:6490728/150‑1 (MQ=255)

                                                                            cGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGAc                                                                           <  1:1378239/150‑1 (MQ=255)

                                                                             gAAATCGTTGCTGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACg                                                                          <  1:5063179/150‑1 (MQ=255)

                                                                             gAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACg                                                                          <  2:541941/150‑1 (MQ=255)

                                                                             gAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACg                                                                          >  1:6704624/1‑150 (MQ=255)

                                                                                aTCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTc                                                                       <  1:7748278/150‑1 (MQ=255)

                                                                                aTCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTc                                                                       <  2:8100889/150‑1 (MQ=255)

                                                                                   gTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTc                                                                    >  1:917331/1‑150 (MQ=255)

                                                                                     tGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCaa                                                                  <  2:5228618/150‑1 (MQ=255)

                                                                                      gATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGa                                                                            <  2:8609958/139‑1 (MQ=255)

                                                                                      gATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAg                                                                 >  1:8782450/1‑150 (MQ=255)

                                                                                      gATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAg                                                                 >  1:3266081/1‑150 (MQ=255)

                                                                                             cTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACgg                                                          <  2:1120891/150‑1 (MQ=255)

                                                                                               gAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCt                                                        <  1:5347213/150‑1 (MQ=255)

                                                                                                aaCTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTg                                                       >  1:8720518/1‑150 (MQ=255)

                                                                                                   tGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCa                                                    >  2:4210494/1‑150 (MQ=255)

                                                                                                   tGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCa                                                    <  2:7544944/150‑1 (MQ=255)

                                                                                                   tGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCGGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCa                                                    >  1:5539326/1‑150 (MQ=255)

                                                                                                    gCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCAt                                                   >  1:172736/1‑150 (MQ=255)

                                                                                                      aGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATcc                                                 >  2:2411750/1‑150 (MQ=255)

                                                                                                           gACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGcc                                            <  2:4366456/150‑1 (MQ=255)

                                                                                                           gACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGcc                                            >  2:4894580/1‑150 (MQ=255)

                                                                                                              ggCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGaa                                         <  1:8598565/150‑1 (MQ=255)

                                                                                                                 cAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGAGGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTc                                      >  2:6713542/1‑150 (MQ=255)

                                                                                                                           tCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCccagcacc                            >  2:4015152/1‑150 (MQ=255)

                                                                                                                           tCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCccagcacc                            >  2:6575043/1‑150 (MQ=255)

                                                                                                                            ccGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCccagcacca                           <  1:1968023/150‑1 (MQ=255)

                                                                                                                                 gCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATc                      <  1:3415084/150‑1 (MQ=255)

                                                                                                                                     tGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTtgt                  >  2:4647546/1‑150 (MQ=255)

                                                                                                                                     tGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTtgt                  <  1:4663659/150‑1 (MQ=255)

                                                                                                                                         gAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCgg              <  1:3918686/150‑1 (MQ=255)

                                                                                                                                         gAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCgg              >  1:643048/1‑150 (MQ=255)

                                                                                                                                         gAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCgg              >  2:4618821/1‑150 (MQ=255)

                                                                                                                                          aGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGc             <  1:371559/150‑1 (MQ=255)

                                                                                                                                           gCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCa            <  1:6894031/150‑1 (MQ=255)

                                                                                                                                            ccTGGACGTGACGGTACCGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAg           >  1:1153957/1‑150 (MQ=18)

                                                                                                                                            ccTGGACGTGACGGTACCGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAg           >  1:8178637/1‑150 (MQ=18)

                                                                                                                                             cTGGACGTGACGGTACCGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGc          >  1:3539081/1‑150 (MQ=18)

                                                                                                                                             cTGGACGTGACGGTACCGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGc          >  1:1776141/1‑150 (MQ=18)

                                                                                                                                             cTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGc          <  2:4564364/150‑1 (MQ=255)

                                                                                                                                              tGGACGGTACCGTCACGTCCAGGCTCACAGTGGCGTCGTTCAACACGCGGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCAGCTTGGGCGGCAGCt         >  2:3553738/1‑150 (MQ=255)

                                                                                                                                                gACGGTACCGTCACGTCCAGGCTCGCATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTcc       >  2:1291214/1‑150 (MQ=255)

                                                                                                                                                gACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTcc       <  1:7851019/150‑1 (MQ=255)

                                                                                                                                                   ggTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCCAcc    >  2:6190559/1‑150 (MQ=255)

                                                                                                                                                   ggTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCCAcc    >  2:5991077/1‑150 (MQ=255)

TTCAGGGCGACCAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCCACCTC  >  NC_002947/3005608‑3005906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`