Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,015,961 A→C 6.0% V10V (GTA→GTC)  bcsG → endoglucanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,015,9610AC 6.0% 1256.7 / 44.8 371V10V (GTA→GTC) bcsGendoglucanase
Reads supporting (aligned to +/- strand):  ref base A (174/173);  new base C (5/18);  total (179/192)
Fisher's exact test for biased strand distribution p-value = 9.22e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 373 total aligned reads displayed.

TGGCCTTGTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCG  >  NC_002947/3015812‑3016110
                                                                                                                                                     |                                                                                                                                                     
tggcctTGTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTa                                                                                                                                                       <  2:7764562/150‑1 (MQ=255)
 ggcctTGTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTAc                                                                                                                                                      >  2:2815617/1‑150 (MQ=255)
  gcctTGTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACt                                                                                                                                                     >  1:4853376/1‑150 (MQ=255)
  gcctTGTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACt                                                                                                                                                     >  2:1804164/1‑150 (MQ=255)
      tgttgCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGcc                                                                                                                                                 <  2:464905/150‑1 (MQ=255)
       gttgCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGccc                                                                                                                                                <  2:7585746/150‑1 (MQ=255)
       gttgCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGccc                                                                                                                                                <  1:321879/150‑1 (MQ=255)
         tgCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGc                                                                                                                                              <  2:3477419/150‑1 (MQ=255)
           cTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTg                                                                                                                                            >  2:1016356/1‑150 (MQ=255)
             aGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGc                                                                                                                                          <  1:8404598/150‑1 (MQ=255)
               tGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCt                                                                                                                                        <  1:5770266/150‑1 (MQ=255)
               tGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCt                                                                                                                                        >  1:5018637/1‑150 (MQ=255)
               tGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCt                                                                                                                                        <  2:4347568/150‑1 (MQ=255)
                gcgcCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTg                                                                                                                                       >  2:7685950/1‑150 (MQ=255)
                  gcCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGa                                                                                                                                     >  2:6825699/1‑150 (MQ=255)
                    cTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGccagtacc                                                                                                                                   <  2:1879033/150‑7 (MQ=255)
                    cTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGccagtacc                                                                                                                                   <  1:2076813/150‑7 (MQ=255)
                     tgtgTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTg                                                                                                                                  >  1:989943/1‑150 (MQ=255)
                     tgtgTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGccagtaccg                                                                                                                                  <  2:5033776/150‑8 (MQ=255)
                       tgtTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTggg                                                                                                                                <  2:4930418/150‑1 (MQ=255)
                       tgtTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTggg                                                                                                                                <  2:2071898/150‑1 (MQ=255)
                        gtTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGa                                                                                                                               <  1:7171930/150‑1 (MQ=38)
                        gtTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCACCACCCTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGa                                                                                                                               <  2:6506225/150‑1 (MQ=38)
                          tCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGACACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCCGAATGACTACTACCACCATCACCCTCCCCGTCCAGGCCAGCGGGCCAGTACCGGGAgg                                                                                                                             <  1:3756062/150‑1 (MQ=38)
                          tCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAgg                                                                                                                             <  2:7795451/150‑1 (MQ=255)
                           cGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAggg                                                                                                                            <  1:2365119/150‑1 (MQ=255)
                            ggCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGt                                                                                                                           <  2:4172878/150‑1 (MQ=255)
                            ggCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGt                                                                                                                           <  2:5604369/150‑1 (MQ=255)
                            ggCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGc                                                                                                                                          >  1:1864489/1‑135 (MQ=255)
                             gCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTg                                                                                                                          <  2:6340605/150‑1 (MQ=255)
                             gCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTg                                                                                                                          >  2:7232072/1‑150 (MQ=255)
                               aTTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGa                                                                                                                        <  1:1749825/150‑1 (MQ=255)
                                ttACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCGTCCCGGTACTGGCCCGCTGGCGTGGACTGGGAGGGTGGaa                                                                                                                       >  2:7625848/1‑150 (MQ=255)
                                ttACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGaa                                                                                                                       >  1:361958/1‑150 (MQ=255)
                                ttACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGaa                                                                                                                       >  2:7688699/1‑150 (MQ=255)
                                    gTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACctc                                                                                                                   <  2:3407584/150‑1 (MQ=255)
                                    gTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACctc                                                                                                                   >  2:1966875/1‑150 (MQ=255)
                                      ggCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTa                                                                                                                 <  1:6350448/150‑1 (MQ=255)
                                            ttGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAtttttt                                                                                                           >  2:1144799/1‑150 (MQ=255)
                                             tGCGCCGTCGCCTTCCCCCCCGTGACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAttttttt                                                                                                          <  2:3311411/150‑1 (MQ=255)
                                                 ccGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCaa                                                                                                      >  2:4556906/1‑150 (MQ=255)
                                                 ccGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCaa                                                                                                      >  2:1679594/1‑150 (MQ=255)
                                                       ccTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTc                                                                                                >  1:7340884/1‑150 (MQ=255)
                                                       ccTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTc                                                                                                >  1:2898496/1‑150 (MQ=255)
                                                       ccTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTc                                                                                                >  2:2947543/1‑150 (MQ=255)
                                                        cTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTct                                                                                               >  1:3427681/1‑150 (MQ=255)
                                                            ccccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTctgctg                                                                                           <  1:6056341/150‑1 (MQ=255)
                                                              ccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGt                                                                                         >  1:7823046/1‑150 (MQ=255)
                                                              ccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTGTCTGCTGGt                                                                                         >  1:4074748/1‑150 (MQ=255)
                                                               cccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTc                                                                                        >  1:7931085/1‑150 (MQ=255)
                                                                      ccTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGTCTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTgg                                                                                 <  2:7967616/150‑1 (MQ=255)
                                                                      ccTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTgg                                                                                 <  1:747248/150‑1 (MQ=255)
                                                                      ccTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTgg                                                                                 <  2:4944268/150‑1 (MQ=255)
                                                                      ccTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTgg                                                                                 >  1:4790780/1‑150 (MQ=255)
                                                                      ccTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTgg                                                                                 <  1:495543/150‑1 (MQ=255)
                                                                      ccTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTgg                                                                                 <  2:4040035/150‑1 (MQ=255)
                                                                       cTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGACCCTCTATTTTTTTGCACAGTTTCTGATGGTCGCGCTggg                                                                                >  2:8363362/1‑150 (MQ=255)
                                                                        tGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGc                                                                               >  2:2233716/1‑150 (MQ=255)
                                                                          aaaCCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCAt                                                                             <  1:526991/150‑1 (MQ=255)
                                                                            aCCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGc                                                                           >  2:522549/1‑150 (MQ=255)
                                                                            aCCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGc                                                                           >  1:7289748/1‑150 (MQ=255)
                                                                              cGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGACTGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTc                                                                         <  2:257453/150‑1 (MQ=255)
                                                                               gCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGCACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCg                                                                        >  1:6656141/1‑150 (MQ=255)
                                                                                      gTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCa                                                                 <  2:8514368/150‑1 (MQ=255)
                                                                                      gTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCa                                                                 >  1:5677073/1‑150 (MQ=255)
                                                                                      gTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCa                                                                 <  1:1508123/150‑1 (MQ=255)
                                                                                        aCGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAgg                                                               >  2:8538076/1‑150 (MQ=255)
                                                                                           ccgccgcGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGgcgc                                                            <  1:2815617/150‑1 (MQ=255)
                                                                                           ccgccgcGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGgcgc                                                            <  1:4244381/150‑1 (MQ=255)
                                                                                                    tGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAAcc                                                   >  1:6973025/1‑150 (MQ=255)
                                                                                                        cGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCtgtt                                               <  2:2846122/150‑1 (MQ=255)
                                                                                                        cGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCtgtt                                               <  1:8138339/150‑1 (MQ=255)
                                                                                                        cGCCAGGAACCCCAGAATGACGAATACCAACATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTGTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCtgtt                                               <  1:8612945/150‑1 (MQ=255)
                                                                                                            aGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTc                                           >  1:4502041/1‑150 (MQ=255)
                                                                                                                     aGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGttt                                  <  1:6775746/150‑1 (MQ=255)
                                                                                                                         tGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGc                              <  1:4640226/150‑1 (MQ=255)
                                                                                                                             tAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTGTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGt                          >  2:5940647/1‑150 (MQ=255)
                                                                                                                              aaTACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTc                         <  2:4746050/150‑1 (MQ=255)
                                                                                                                                 accaccATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCt                      >  2:3076948/1‑150 (MQ=255)
                                                                                                                                 accaccATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCt                      >  2:663295/1‑150 (MQ=255)
                                                                                                                                  ccaccaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCtt                     >  1:1686296/1‑150 (MQ=255)
                                                                                                                                    accaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTg                   <  2:5402726/150‑1 (MQ=255)
                                                                                                                                     ccaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGc                  <  2:3644932/150‑1 (MQ=255)
                                                                                                                                      caTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGcc                 >  2:1453367/1‑150 (MQ=255)
                                                                                                                                       aTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCg                >  1:6179703/1‑150 (MQ=255)
                                                                                                                                          aCCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGc             <  2:8728290/150‑1 (MQ=255)
                                                                                                                                          aCCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGc             >  2:3947283/1‑150 (MQ=255)
                                                                                                                                           cccTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCa            >  1:5559534/1‑150 (MQ=255)
                                                                                                                                            ccTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCaa           >  1:3305907/1‑150 (MQ=255)
                                                                                                                                             cTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAg          >  2:629201/1‑150 (MQ=255)
                                                                                                                                             cTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAg          >  1:936029/1‑150 (MQ=255)
                                                                                                                                              tCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGt         <  2:1346137/150‑1 (MQ=255)
                                                                                                                                              tCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTATTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGt         <  1:1262148/150‑1 (MQ=255)
                                                                                                                                                ccGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAAGCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTGGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTgg       >  1:183886/1‑150 (MQ=255)
                                                                                                                                                ccGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTgg       >  1:5940585/1‑150 (MQ=255)
                                                                                                                                                 cGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGc      >  2:4849109/1‑150 (MQ=255)
                                                                                                                                                 cGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGc      >  2:8651122/1‑150 (MQ=255)
                                                                                                                                                  ggTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCt     <  2:7079940/150‑1 (MQ=255)
                                                                                                                                                    tACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGc   <  1:1192571/150‑1 (MQ=255)
                                                                                                                                                     aCTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCg  >  2:3838700/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGCCTTGTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCG  >  NC_002947/3015812‑3016110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑