Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,015,968 C→A 6.4% R13S (CGC→AGC)  bcsG → endoglucanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,015,9680CA 6.4% 1235.6 / 49.9 368R13S (CGC→AGC) bcsGendoglucanase
Reads supporting (aligned to +/- strand):  ref base C (171/173);  new base A (6/18);  total (177/191)
Fisher's exact test for biased strand distribution p-value = 2.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 377 total aligned reads displayed.

GTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCGTATTGCC  >  NC_002947/3015819‑3016117
                                                                                                                                                     |                                                                                                                                                     
gttgCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGccc                                                                                                                                                       <  2:7585746/150‑1 (MQ=255)
   gCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCt                                                                                                                                                    <  2:4107035/150‑1 (MQ=255)
    cTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTg                                                                                                                                                   >  1:7315513/1‑150 (MQ=255)
      aGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGc                                                                                                                                                 <  1:8404598/150‑1 (MQ=255)
        tGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCt                                                                                                                                               <  2:4347568/150‑1 (MQ=255)
         gcgcCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTg                                                                                                                                              <  2:6436377/150‑1 (MQ=255)
         gcgcCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCGGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGccag                                                                                                                                              <  2:7781191/150‑3 (MQ=255)
             cTGTGTTCGGCATTTCGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCTCCGCGCTTCTCGCCAGGAACCCCTGAATGACTAATACCAACATCACCCTCCCGTTACTGGCCCGATGGCCTTGACt                                                                                                                                          <  2:4689914/150‑1 (MQ=255)
             cTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGccagtacc                                                                                                                                          <  1:2076813/150‑7 (MQ=255)
             cTGTGTTCGGCAGTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGccagtacc                                                                                                                                          <  2:1925239/150‑7 (MQ=255)
              tgtgTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCCGGAACCCCAGAATGACTAATACCACCATCACCCACCCGGTACTGGCCCGCTGGCCTGGACTg                                                                                                                                         >  2:8525013/1‑150 (MQ=255)
              tgtgTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTg                                                                                                                                         >  2:7931076/1‑150 (MQ=255)
              tgtgTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTg                                                                                                                                         >  1:989950/1‑150 (MQ=255)
              tgtgTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTg                                                                                                                                         <  1:4176920/150‑1 (MQ=255)
              tgtgTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGccagtaccg                                                                                                                                         <  2:2534116/150‑8 (MQ=255)
              tgtgTTCGGCAGTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGccagtaccg                                                                                                                                         <  2:8275522/150‑8 (MQ=255)
               gtgtTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGATCCCCAGAATGTCTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTgg                                                                                                                                        <  2:6715423/150‑1 (MQ=255)
                tgtTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTggg                                                                                                                                       <  2:4930544/150‑1 (MQ=255)
                 gtTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGa                                                                                                                                      <  2:7059019/150‑1 (MQ=255)
                 gtTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGa                                                                                                                                      <  2:3846044/150‑1 (MQ=38)
                 gtTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGa                                                                                                                                      <  1:7172164/150‑1 (MQ=38)
                  ttCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGAg                                                                                                                                     <  2:8654915/150‑1 (MQ=38)
                   tCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAgg                                                                                                                                    <  2:7165814/150‑1 (MQ=255)
                   tCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAgg                                                                                                                                    <  2:771747/150‑1 (MQ=255)
                   tCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAgg                                                                                                                                    <  2:4840531/150‑1 (MQ=255)
                   tCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAgg                                                                                                                                    <  1:5595393/150‑1 (MQ=255)
                     ggCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGt                                                                                                                                  <  1:3613822/150‑1 (MQ=255)
                     ggCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGc                                                                                                                                                 >  1:1864489/1‑135 (MQ=255)
                      gCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTg                                                                                                                                 <  2:6340605/150‑1 (MQ=255)
                      gCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTg                                                                                                                                 >  2:1317798/1‑150 (MQ=255)
                      gCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGACGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTg                                                                                                                                 >  1:3713373/1‑150 (MQ=255)
                       cATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTgg                                                                                                                                >  2:6604424/1‑150 (MQ=255)
                         ttACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGac                                                                                                                              >  2:7688296/1‑149 (MQ=255)
                         ttACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGaa                                                                                                                              <  1:338588/150‑1 (MQ=255)
                             gTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACctc                                                                                                                          >  2:1967025/1‑150 (MQ=255)
                              tGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACctct                                                                                                                         >  1:1762677/1‑150 (MQ=255)
                                gCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAt                                                                                                                       >  1:453559/1‑150 (MQ=255)
                                  gtgtTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAttt                                                                                                                     <  1:4750779/150‑1 (MQ=255)
                                      tGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAttttttt                                                                                                                 <  1:5149250/150‑1 (MQ=255)
                                        cgcCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGc                                                                                                               <  2:4596530/150‑1 (MQ=255)
                                          ccGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCaa                                                                                                             >  2:1679594/1‑150 (MQ=255)
                                          ccGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCaa                                                                                                             >  2:6186167/1‑150 (MQ=255)
                                                ccTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTc                                                                                                       >  1:6001766/1‑150 (MQ=255)
                                                 cTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCGCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTct                                                                                                      <  2:3008942/150‑1 (MQ=255)
                                                    cccccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTctgct                                                                                                   <  2:2136534/150‑1 (MQ=255)
                                                     ccccccGGTCCCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGACCCTCTATTTTTTTGCAAAGTGTctgctg                                                                                                  >  1:2886369/1‑150 (MQ=255)
                                                       ccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGt                                                                                                >  1:7823046/1‑150 (MQ=255)
                                                       ccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCGGGACTGGGAGGGTGGACCCTCTATTTTTTTGCAAAGTTTCTGCTGGt                                                                                                >  1:8551316/1‑150 (MQ=255)
                                                          cGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTcgc                                                                                             <  1:6295071/150‑1 (MQ=255)
                                                               ccTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTgg                                                                                        <  1:5351919/150‑1 (MQ=255)
                                                                cTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTggg                                                                                       >  2:8576647/1‑150 (MQ=255)
                                                                cTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTggg                                                                                       >  2:8423827/1‑150 (MQ=255)
                                                                cTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTggg                                                                                       >  2:5709541/1‑150 (MQ=255)
                                                                 tGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGc                                                                                      >  1:7626217/1‑150 (MQ=255)
                                                                     aCCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGc                                                                                  >  2:522215/1‑150 (MQ=255)
                                                                       cGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTc                                                                                <  1:8749782/150‑1 (MQ=255)
                                                                       cGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGGACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTc                                                                                <  1:7369655/150‑1 (MQ=255)
                                                                            ggCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCACAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACtt                                                                           <  2:7658411/150‑1 (MQ=255)
                                                                              cGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTcc                                                                         <  1:3066723/150‑1 (MQ=255)
                                                                               gTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCa                                                                        <  1:1508123/150‑1 (MQ=255)
                                                                                 gcgccgccgcGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGa                                                                                                                                      <  2:558137/85‑1 (MQ=255)
                                                                                            cTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAAc                                                           <  1:4282783/150‑1 (MQ=255)
                                                                                                aCGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCtgt                                                       >  1:6112530/1‑150 (MQ=255)
                                                                                                 cGCCAGGAACCCCAGAATGACTAATACCACCATCGCCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCtgtt                                                      >  1:6695825/1‑150 (MQ=255)
                                                                                                 cGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCtgtt                                                      <  1:8138339/150‑1 (MQ=255)
                                                                                                     aGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTc                                                  >  2:5008224/1‑150 (MQ=255)
                                                                                                      ggAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCg                                                 >  2:3472189/1‑150 (MQ=255)
                                                                                                        aaCCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCACAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCt                                               <  2:3317569/150‑1 (MQ=255)
                                                                                                        aaCCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCt                                               <  2:2659622/150‑1 (MQ=255)
                                                                                                           cccAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCg                                            >  1:4961371/1‑150 (MQ=255)
                                                                                                           cccAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGGTGTTCGCTGCg                                            >  2:2889956/1‑150 (MQ=255)
                                                                                                             cAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGtt                                          >  2:3225647/1‑150 (MQ=255)
                                                                                                                caTGACTAAGACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTCTTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCt                                       <  2:1119350/149‑1 (MQ=255)
                                                                                                                 aTGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTg                                      <  1:7931076/150‑1 (MQ=255)
                                                                                                                       aaTACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTc                                <  1:3289628/150‑1 (MQ=255)
                                                                                                                        aTACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTcc                               >  2:419727/1‑150 (MQ=255)
                                                                                                                          accaccATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCt                             >  2:1826525/1‑150 (MQ=255)
                                                                                                                          accaccATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCt                             <  2:1050946/150‑1 (MQ=255)
                                                                                                                           ccaccaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCtt                            >  1:1686296/1‑150 (MQ=255)
                                                                                                                             accaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTg                          <  2:5402726/150‑1 (MQ=255)
                                                                                                                             accaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTg                          <  1:6988848/150‑1 (MQ=255)
                                                                                                                               caTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGcc                        >  2:1453367/1‑150 (MQ=255)
                                                                                                                                aTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCg                       <  1:5684327/150‑1 (MQ=255)
                                                                                                                                 tCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCgg                      <  1:4892387/150‑1 (MQ=255)
                                                                                                                                  cACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCggg                     <  2:2833453/150‑1 (MQ=255)
                                                                                                                                   aCCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGc                    >  2:3947283/1‑150 (MQ=255)
                                                                                                                                   aCCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGc                    >  1:5904130/1‑150 (MQ=255)
                                                                                                                                     ccTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCaa                  <  2:377229/150‑1 (MQ=255)
                                                                                                                                       tCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGt                >  1:6397696/1‑150 (MQ=255)
                                                                                                                                       tCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTATTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGt                <  1:1262148/150‑1 (MQ=255)
                                                                                                                                         ccGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAAGCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTGGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTgg              >  1:183886/1‑150 (MQ=255)
                                                                                                                                         ccGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCaatacg              >  1:2644630/1‑146 (MQ=255)
                                                                                                                                           ggTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCt            >  1:6855402/1‑150 (MQ=255)
                                                                                                                                             tACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGc          <  1:1192571/150‑1 (MQ=255)
                                                                                                                                               ttGGCCAGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCGt        >  2:3568022/2‑150 (MQ=255)
                                                                                                                                                tGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGTCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCGTa       >  1:47972/1‑150 (MQ=255)
                                                                                                                                                tGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCGTa       >  1:3964216/1‑150 (MQ=255)
                                                                                                                                                tGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCGTa       >  1:2375287/1‑150 (MQ=255)
                                                                                                                                                     cGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCGTATTGcc  <  2:7315513/150‑1 (MQ=255)
                                                                                                                                                     cGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCGTATTGcc  <  2:486841/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCGTATTGCC  >  NC_002947/3015819‑3016117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑