Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,419,586 C→T 6.0% A535V (GCC→GTC)  PP_3026 → recombinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,419,5860CT 6.0% 831.6 / 27.5 264A535V (GCC→GTC) PP_3026recombinase
Reads supporting (aligned to +/- strand):  ref base C (105/143);  new base T (6/10);  total (111/153)
Fisher's exact test for biased strand distribution p-value = 7.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 286 total aligned reads displayed.

ATGATCGATACTTTCGATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATACACGCCAGGCGGCCCATGCGACA  >  NC_002947/3419438‑3419735
                                                                                                                                                    |                                                                                                                                                     
    tCGATACTTTCGATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCg                                                                                                                                                  >  1:4532052/1‑150 (MQ=255)
    tCGATACTTTCGATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCg                                                                                                                                                  <  2:3465302/150‑1 (MQ=255)
     cGATACTTTCGATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGt                                                                                                                                                 >  2:7323673/1‑150 (MQ=255)
      gATACTTTCGATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTc                                                                                                                                                <  1:5692426/150‑1 (MQ=255)
      gATACTTTCGATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTc                                                                                                                                                >  2:8473122/1‑150 (MQ=255)
       aTACTTTCGATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGTCGACGCCg                                                                                                                                               <  2:1582646/150‑1 (MQ=255)
       aTACTTTCGATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCg                                                                                                                                               >  1:7936488/1‑150 (MQ=255)
        tACTTTCGATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGa                                                                                                                                              <  1:1472589/150‑1 (MQ=255)
        tACTTTCGATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGa                                                                                                                                              <  1:1923025/150‑1 (MQ=255)
          cTTTCGATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGAcc                                                                                                                                            <  2:5670040/150‑1 (MQ=255)
           tttCGATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCg                                                                                                                                           >  1:5062087/1‑150 (MQ=255)
              cGATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGTCGACGCCGGCCGccc                                                                                                                                        <  1:3862352/150‑1 (MQ=255)
               gATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGTCGACGCCGGCCGCCCa                                                                                                                                       <  2:1219532/150‑1 (MQ=255)
               gATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGTCGACGCCGGCCGCCCa                                                                                                                                       <  2:5824430/150‑1 (MQ=255)
               gATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCa                                                                                                                                       <  2:3837049/150‑1 (MQ=255)
               gATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCa                                                                                                                                       <  1:486267/150‑1 (MQ=255)
                aTCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAg                                                                                                                                      >  2:8778726/1‑150 (MQ=255)
                aTCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAg                                                                                                                                      >  1:4154098/1‑150 (MQ=255)
                      tCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGAt                                                                                                                                <  1:3216077/150‑1 (MQ=255)
                      tCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGAt                                                                                                                                >  1:6289124/1‑150 (MQ=255)
                         tGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGtt                                                                                                                             >  2:690138/1‑150 (MQ=255)
                         tGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGtt                                                                                                                             >  1:2993493/1‑150 (MQ=255)
                            tttACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTAc                                                                                                                          <  1:525685/150‑1 (MQ=255)
                            tttACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTAc                                                                                                                          >  2:1528684/1‑150 (MQ=255)
                              tACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGc                                                                                                                        <  2:5977384/150‑1 (MQ=255)
                               aCATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCt                                                                                                                       <  2:2094073/150‑1 (MQ=255)
                                 aTGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGa                                                                                                                     <  2:5913957/150‑1 (MQ=255)
                                        gtgtAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTg                                                                                                              >  2:7592444/1‑150 (MQ=255)
                                          gtAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGAc                                                                                                                    <  1:4526176/142‑1 (MQ=255)
                                          gtAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGAc                                                                                                                    >  2:4526176/1‑142 (MQ=255)
                                          gtAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTgtc                                                                                                            <  2:4499415/150‑1 (MQ=255)
                                          gtAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTgtc                                                                                                            <  2:8344954/150‑1 (MQ=255)
                                           tAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTgtcg                                                                                                           >  2:2580952/1‑150 (MQ=255)
                                           tAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTgtcg                                                                                                           >  1:82591/1‑150 (MQ=255)
                                            aGTGCCAGCGGGGCGCAGGGCCAGGTAAATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCTGCGATGGCTCAGGGTAGAAAGTAAGACCGGAGAGTTGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACTCTGACTTCTTgtcgt                                                                                                          <  2:374568/150‑1 (MQ=255)
                                             gTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGa                                                                                                                                     <  1:3803170/122‑1 (MQ=255)
                                             gTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTgtcgtc                                                                                                         >  1:697276/1‑150 (MQ=255)
                                                 cAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCACGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCGGACTTCTTGTCGTCTAtt                                                                                                     <  2:6382858/150‑1 (MQ=255)
                                                  aGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTg                                                                                                    <  1:892835/150‑1 (MQ=255)
                                                  aGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTg                                                                                                    >  1:1035323/1‑150 (MQ=255)
                                                   gCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTgg                                                                                                   <  2:5202741/150‑1 (MQ=255)
                                                     ggggCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGa                                                                                                 <  1:1321196/150‑1 (MQ=255)
                                                       ggCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAgg                                                                                               >  2:1667426/1‑150 (MQ=255)
                                                           cAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCgg                                                                                           <  1:1156047/150‑1 (MQ=255)
                                                           cAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCgg                                                                                           <  2:6164630/150‑1 (MQ=255)
                                                           cAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCgg                                                                                           >  1:6537763/1‑150 (MQ=255)
                                                               gCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTgg                                                                                       <  1:8346928/150‑1 (MQ=255)
                                                                 cAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGc                                                                                     <  1:1379866/150‑1 (MQ=255)
                                                                    gTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAg                                                                                  <  2:2223441/150‑1 (MQ=255)
                                                                     tACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAgt                                                                                 >  1:80777/1‑150 (MQ=255)
                                                                         tCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGc                                                                             >  1:1685449/1‑150 (MQ=255)
                                                                               tCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCaccacc                                                                       >  1:237253/1‑150 (MQ=255)
                                                                                 ttGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAg                                                                     <  1:7441671/150‑1 (MQ=255)
                                                                                      gATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGc                                                                >  1:4464807/1‑150 (MQ=255)
                                                                                       atgtCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGcc                                                               <  1:539245/147‑1 (MQ=255)
                                                                                       aTTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGcc                                                               <  1:2580952/150‑1 (MQ=255)
                                                                                           ccAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGt                                                           <  1:2827123/150‑1 (MQ=255)
                                                                                           ccAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCGATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGt                                                           >  2:299118/1‑150 (MQ=255)
                                                                                            cAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGtc                                                          <  1:4752757/150‑1 (MQ=255)
                                                                                             aaGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGtcc                                                         >  2:7834940/1‑150 (MQ=255)
                                                                                             aaGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGtcc                                                         >  1:5796891/1‑150 (MQ=255)
                                                                                             aaGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGtcc                                                         <  2:1136483/150‑1 (MQ=255)
                                                                                             aaGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGAACCACCAGGTGGCCTTGtcc                                                         >  2:6542825/1‑150 (MQ=255)
                                                                                               ggCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGtccct                                                       >  1:6052375/1‑150 (MQ=255)
                                                                                               ggCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGGCGTCTATTGGGAGGCCGGGTGGGCTCGTGTGCACCACCAGGTGGCCTTGtccct                                                       >  1:7538332/1‑150 (MQ=255)
                                                                                                gCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGtccctc                                                      >  1:4392497/1‑150 (MQ=255)
                                                                                                 cAGGACAGCGGCGATGGCTCAGGTTAGAAAGTAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCACAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGTGAGGCCGGGTTGGCTAGTGTGCACCACCAGGTGGCCTTGtccctcc                                                     <  1:7758197/150‑1 (MQ=255)
                                                                                                 cAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGtccctcc                                                     <  1:5822573/150‑1 (MQ=255)
                                                                                                  aGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGtccctccc                                                    >  2:8062397/1‑150 (MQ=255)
                                                                                                  aGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGtccctccc                                                    >  1:7127441/1‑150 (MQ=255)
                                                                                                       aGCGGCGAGGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGTCCGCCCAGACTGTTGTTTACGCTGACTTCTTGTCGTCTATTGGGTGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGaaa                                               <  2:6502180/150‑1 (MQ=255)
                                                                                                        gcggcgATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAAt                                              <  2:4784224/150‑1 (MQ=255)
                                                                                                         cggcgATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAAtt                                             <  1:226094/150‑1 (MQ=255)
                                                                                                          ggcgATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAAttt                                            >  1:3105709/1‑150 (MQ=255)
                                                                                                           gcgATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAAtttt                                           >  2:8576823/1‑150 (MQ=255)
                                                                                                            cgATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTc                                          <  1:3321083/150‑1 (MQ=255)
                                                                                                            cgATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTc                                          <  2:8690911/150‑1 (MQ=255)
                                                                                                            cgATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTc                                          <  2:5062087/150‑1 (MQ=255)
                                                                                                             gATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTcc                                         <  2:4059216/150‑1 (MQ=255)
                                                                                                             gATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTcc                                         <  2:3160267/150‑1 (MQ=255)
                                                                                                                  cTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTAc                                    <  1:6190571/150‑1 (MQ=255)
                                                                                                                           gAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCa                           >  2:1614488/1‑150 (MQ=255)
                                                                                                                           gAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCa                           >  2:4304243/1‑150 (MQ=255)
                                                                                                                             aaGGAAGACCGGAGAGTGGGCGGCTGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATa                         >  2:7250102/1‑150 (MQ=255)
                                                                                                                             aaGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATa                         <  1:2902890/150‑1 (MQ=255)
                                                                                                                             aaGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATa                         <  1:5449939/150‑1 (MQ=255)
                                                                                                                                gAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATacac                      <  1:1559052/150‑1 (MQ=255)
                                                                                                                                    aCCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATACACGCCa                  <  1:7323673/150‑1 (MQ=255)
                                                                                                                                       ggAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCAGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATACACGCCAggc               >  1:5138447/1‑150 (MQ=255)
                                                                                                                                        gagaGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATACACGCCAggcg              >  2:5764661/1‑150 (MQ=255)
                                                                                                                                        gagaGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATACACGCCAggcg              <  2:7154527/150‑1 (MQ=255)
                                                                                                                                        gagaGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATACACGCCAggcg              <  2:2326748/150‑1 (MQ=255)
                                                                                                                                          gagTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATACACGCCAggcggc            <  1:4023824/150‑1 (MQ=255)
                                                                                                                                             tGGGCGGTCGACGCCGGCCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATACACGCCAGGCGGCCCa         >  2:8110490/1‑150 (MQ=255)
                                                                                                                                              gggCGGTCGACGCCGGCCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATACACGCCAGGCGGCCCAt        >  1:8498496/1‑150 (MQ=255)
                                                                                                                                              gggCGGTCGACGCCGGCCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATACACGCCAGGCGGCCCAt        >  1:4674770/1‑150 (MQ=255)
                                                                                                                                               ggcggtCGACGCCGGCCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATACACGCCAGGCGGCCCATg       >  1:5470507/1‑150 (MQ=255)
                                                                                                                                               ggcggcCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATACACGCCAGGCGGCCCATg       >  1:7786715/1‑150 (MQ=255)
                                                                                                                                               ggcggcCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATACACGCCAGGCGGCCCATg       <  2:2993494/150‑1 (MQ=255)
                                                                                                                                                gcggcCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATACACGCCAGGCGGCCCATGc      <  2:4154098/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATGATCGATACTTTCGATCGCATCGTGATTTACATGCGTGGTGTAGTGCCAGCGGGGCGCAGGGCCAGGTACATCGATGTCTTGCTGATTTCCAAGGCAGGACAGCGGCGATGGCTCAGGGTAGAAAGGAAGACCGGAGAGTGGGCGGCCGGCGTCGACCGCCCAGACTGATGTTTACGCTGACTTCTTGTCGTCTATTGGGAGGCCGGGTGGGCTAGTGTGCACCACCAGGTGGCCTTGTCCCTCCCAGAAATTTTCCTGTACCTCGACGCATACACGCCAGGCGGCCCATGCGACA  >  NC_002947/3419438‑3419735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑