BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,497,082	A→T	5.2%	L110Q (CTG→CAG)	PP_3096 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,497,082	0	A	T	5.2%	925.4 / 11.1	301	L110Q (CTG→CAG)	PP_3096	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (174/94); new base T (4/17); total (178/123)
Fisher's exact test for biased strand distribution p-value = 7.04e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 322 total aligned reads displayed.

CGAAGCCCGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAG  >  NC_002947/3496933‑3497230

cGAAGCCCGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGagcagca                                                                                                                                                       <  2:5255109/150‑1 (MQ=255)

 gAAGCCCGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGagcagcag                                                                                                                                                      >  1:7387455/1‑150 (MQ=255)

  aaGCCCGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGagcagcagc                                                                                                                                                     >  2:8430224/1‑150 (MQ=255)

       cGAGGTGCCAGTGCGCTCGTGCTCGATGTGGAATGTGTATTTTTGCCAGGCCTCGTAGCACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATCCTGCACGTGGCGCTCGAGCAGCAGCTCggg                                                                                                                                                <  2:3070600/150‑2 (MQ=255)

        gAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCTGCTGCTCgagc                                                                                                                                               <  2:6449149/150‑4 (MQ=255)

           gTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTAc                                                                                                                                            <  1:8576934/150‑1 (MQ=255)

             gCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACgg                                                                                                                                          <  1:7498765/150‑1 (MQ=255)

             gCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACgg                                                                                                                                          <  1:7995021/150‑1 (MQ=255)

             gCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACgg                                                                                                                                          <  2:6619204/150‑1 (MQ=255)

               cATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGc                                                                                                                                        <  2:8781839/150‑1 (MQ=255)

                aTTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCg                                                                                                                                       >  1:8684816/1‑150 (MQ=255)

                aTTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCg                                                                                                                                       >  1:4786055/1‑150 (MQ=255)

                 ttGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGt                                                                                                                                      <  1:5583838/150‑1 (MQ=255)

                  tGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTg                                                                                                                                     <  1:1856804/150‑1 (MQ=255)

                    cgcTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGgc                                                                                                                                   <  2:7192347/150‑1 (MQ=255)

                    cgcTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGACATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGgc                                                                                                                                   <  2:500334/150‑1 (MQ=255)

                     gcTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGgc‑a                                                                                                                                 <  1:3125423/150‑1 (MQ=255)

                     gcTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGgc‑a                                                                                                                                 <  2:7569901/150‑1 (MQ=255)

                     gcTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGgc‑a                                                                                                                                 >  2:4735498/1‑150 (MQ=255)

                           gCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGc                                                                                                                           <  1:714101/150‑1 (MQ=255)

                              cGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGCT‑GCCACGCCCg                                                                                                                        <  1:2983525/150‑1 (MQ=255)

                                 tgtgGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACg                                                                                                                     <  2:7901668/150‑1 (MQ=255)

                                  gtgGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACgg                                                                                                                    <  1:5602597/150‑1 (MQ=255)

                                     gAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGccc                                                                                                                 >  2:1579658/1‑150 (MQ=255)

                                     gAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGccc                                                                                                                 >  2:6076245/1‑150 (MQ=255)

                                        tttGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGAc                                                                                                              >  2:3182584/1‑150 (MQ=255)

                                          tGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCa                                                                                                            >  2:6876537/1‑150 (MQ=255)

                                           gTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAg                                                                                                           >  1:2193616/1‑150 (MQ=255)

                                           gTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAg                                                                                                           >  1:7718925/1‑150 (MQ=255)

                                                tttGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCa                                                                                                      >  1:7796190/1‑150 (MQ=255)

                                                   gCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGa                                                                                                   >  2:1093454/1‑150 (MQ=255)

                                                      aGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAgg                                                                                                >  1:6094275/1‑150 (MQ=255)

                                                      aGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAgg                                                                                                >  2:5184906/1‑150 (MQ=255)

                                                       ggCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGACACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGt                                                                                               >  2:1443040/1‑150 (MQ=255)

                                                         ccTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGa                                                                                             >  1:8024591/1‑150 (MQ=255)

                                                          cTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGAc                                                                                            >  2:4715861/1‑150 (MQ=255)

                                                           tCGTAGAACACGGTGGTCTTGGGGTGCGAGAAGATGTGGAGAAACGCTTGGGGGGCTTCGTCGCGATACTGCAGGTGGCGCTCGAGCAGCCGCTCGGTGTAGGGGCGTGGC‑AGCCCGCCCGACGGCCCGACCAGGCCCATGAAGGTGAcc                                                                                           <  2:1793383/150‑1 (MQ=255)

                                                             gTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTg                                                                                         <  2:4193657/150‑1 (MQ=255)

                                                             gTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTg                                                                                         >  2:3795762/1‑150 (MQ=255)

                                                              tAGAACAACGTGGTCTTGCGGTGCGAGAAGTTGTCGACAAACGCTGGGGGGGCATCGTCGCGATCCTGCAGGTGGCGCTCGAGCAGCAGCTGGGTGTCCGGGGGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGc                                                                                        <  2:7424432/150‑1 (MQ=255)

                                                              tAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGc                                                                                        >  1:1631717/1‑150 (MQ=255)

                                                               aGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCa                                                                                       >  2:2011622/1‑150 (MQ=255)

                                                                   caacGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTc                                                                                   >  1:1265892/1‑150 (MQ=255)

                                                                   caacGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTc                                                                                   >  2:2193130/1‑150 (MQ=255)

                                                                   caacGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTc                                                                                   >  2:2809932/1‑150 (MQ=255)

                                                                    aacGTGGTCTTGCGGTGCTATAATATGTCTACAAACGCTTGGGCGGCATCGTCGCGATACTGCAGGTGGCGCTCGCGCCGCAGCTGGGTTTAGGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTcc                                                                                  <  2:73388/150‑1 (MQ=255)

                                                                      ggTGGTCTTGCGGTGCGAGAAGTTGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTGGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGa                                                                                <  2:7780879/149‑1 (MQ=255)

                                                                           tCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCg                                                                           >  2:707220/1‑150 (MQ=255)

                                                                                  gTGCGACAATATGTCCAAAAACGCTTGGGCGCCATCTTCGCTATACGCCACTTGGCCATCTACAAGAAGCTCGGTCAACGGGC‑TGGCAAGCACCCCCCACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAgcc                                                                    <  2:2540544/150‑1 (MQ=255)

                                                                                   tGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAgccg                                                                   >  2:4517342/1‑150 (MQ=255)

                                                                                     cGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAgccggc                                                                 >  1:2846549/1‑150 (MQ=255)

                                                                                        gaagaTGTCGAGAACCGCTTGGGCGGCATCGTCGCGATACGGCACGTGGCGCTCGTGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGccggccgg                                                              <  2:5595703/150‑1 (MQ=255)

                                                                                         aagaTGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGCGCAGCAGCTGGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCcggccggc                                                             <  2:596948/150‑1 (MQ=255)

                                                                                              gTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGacac                                                        >  1:3982526/1‑150 (MQ=255)

                                                                                               tCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACAcc                                                       >  1:210226/1‑150 (MQ=255)

                                                                                                  agaAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTg                                                    >  2:1928551/1‑150 (MQ=255)

                                                                                                  agaAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTg                                                    >  2:2761432/1‑150 (MQ=255)

                                                                                                   gaAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGc                                                   >  1:3416678/1‑150 (MQ=255)

                                                                                                   gaAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGc                                                   >  2:5085006/1‑150 (MQ=255)

                                                                                                    aaaCGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGcc                                                  >  1:6923347/1‑150 (MQ=255)

                                                                                                       cGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTc                                               <  2:5674349/150‑1 (MQ=255)

                                                                                                        gCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTcg                                              >  1:8152081/1‑150 (MQ=255)

                                                                                                          aTGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTcgcg                                            >  1:3273258/1‑150 (MQ=255)

                                                                                                          aTGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTcgcg                                            >  1:2612508/1‑150 (MQ=255)

                                                                                                             gggggcATGTTCGCTATACGGCCGGTGGCGCGCGAGCCGCTGCTGGGTGTACGGGCGTGGC‑CGCCCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTc                                         <  1:6853018/147‑1 (MQ=255)

                                                                                                             ggcggcATCGTCGCGATCCTCAACGTGGCGCTCTAGAACAAGCTGGGTTTAGGGGCGTGCA‑ACCCCCCCCCACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTc                                         <  1:1519365/150‑1 (MQ=255)

                                                                                                             ggcggcATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTc                                         >  2:3415034/1‑150 (MQ=255)

                                                                                                              gcggcATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCg                                        >  2:3873783/1‑150 (MQ=255)

                                                                                                              gcggcATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCg                                        >  1:8162760/1‑150 (MQ=255)

                                                                                                                ggcATCGTCGCTATACTGCAGGTGGCGCTCGAGCCGCCGCTGGGTGTACGGGGGTGGC‑AGCCCGCCCCACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGaa                                      <  2:3688981/150‑1 (MQ=255)

                                                                                                                ggcATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGaa                                      >  2:3622862/1‑150 (MQ=255)

                                                                                                                ggaaTCGGCGCGATACTGCAGGGGGCGCTCGAGCTGCAGCGCGGTGTCGGGGCGTGGC‑AGCCCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGaa                                      <  1:3483737/147‑1 (MQ=255)

                                                                                                                    tcgtcgCGATACTGCAGGTGGCGCTCGCGCCGCCGCGGGGGGTCGGGGCGGGGC‑CGCCCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGc                                  <  2:856870/150‑1 (MQ=255)

                                                                                                                    tcgtcgCGATACTGCACGTGGCGCTCGAGCAGCTGCTCGGTGTAGGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGc                                  <  2:3842436/150‑1 (MQ=255)

                                                                                                                    tcgtcgCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGc                                  <  2:6954907/150‑1 (MQ=255)

                                                                                                                    tcgtcgCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGc                                  >  2:1251673/1‑150 (MQ=255)

                                                                                                                      gtcgCTTTACTGCAGGTGGCGCTGTAGCAGCTGCTGGGTGTAGGGGGGTGGC‑AGCCCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAg                                <  1:4189936/150‑1 (MQ=255)

                                                                                                                       tcgCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAgg                               >  1:4608002/1‑150 (MQ=255)

                                                                                                                         gcgATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTc                             >  1:7525598/1‑150 (MQ=255)

                                                                                                                            aTACGGCACGTGGCGCTCGAGCAGCAGCTGGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCt                          <  1:7312969/150‑1 (MQ=255)

                                                                                                                               aggcccGTGGCGCACGCGACGCAGCTGGGTGTCCGGGCGTGGC‑AGCCCGCCCGACGGCCCGACCAGGCCCATGACGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGAc                       <  1:6553493/145‑1 (MQ=255)

                                                                                                                                tGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGAcc                      <  2:8042206/150‑1 (MQ=255)

                                                                                                                                 gCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCt                     >  1:2374503/1‑150 (MQ=255)

                                                                                                                                    cGTGGGGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACCGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGc                  <  2:7267950/150‑1 (MQ=255)

                                                                                                                                     gTGGCGCGCGAGCAGCTGGTGGGTGTCGGGGCGTGGC‑AGCCCGCCCCACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCt                 <  1:5379753/150‑1 (MQ=255)

                                                                                                                                          gcTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCg            >  1:5881894/1‑150 (MQ=255)

                                                                                                                                          gcTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCg            >  1:4392823/1‑150 (MQ=255)

                                                                                                                                          gcTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCg            >  1:6509881/1‑150 (MQ=255)

                                                                                                                                          gcTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCAGGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCg            >  2:6675838/1‑150 (MQ=255)

                                                                                                                                          ccTCGACAAGCAGCTCGTTGTCGGGGCGTGCC‑ACCCCCCCCCACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCg            <  1:6295683/149‑1 (MQ=255)

                                                                                                                                            tCGCGCAGCTGGTCGGTGTAGGGGGGTGGC‑AGCCCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCggg          <  1:1351604/150‑1 (MQ=255)

                                                                                                                                            ccGAGCTGCTGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCggg          >  1:8414940/2‑150 (MQ=255)

                                                                                                                                             cGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGa         <  2:529232/150‑1 (MQ=255)

                                                                                                                                              gAGCAGCAGCTGGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCcggccggc                                                             <  2:3287547/97‑1 (MQ=255)

                                                                                                                                              gAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGaa        >  1:2259956/1‑150 (MQ=255)

                                                                                                                                              gAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGaa        >  1:8777314/1‑150 (MQ=255)

                                                                                                                                              gAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGaa        >  2:7978566/1‑150 (MQ=255)

                                                                                                                                               agcagcagcTGGGTGTACGGGGGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAAc       <  1:4999426/150‑1 (MQ=255)

                                                                                                                                                   gcagcTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCa   >  1:8271337/1‑150 (MQ=255)

                                                                                                                                                   gcagcTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCa   <  2:4472005/150‑1 (MQ=255)

CGAAGCCCGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGC‑AGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAG  >  NC_002947/3496933‑3497230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`