Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,585,624 C→T 5.3% T256M (ACG→ATG)  benR → BenABC operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,585,6240CT 5.3% 1263.1 / 35.2 392T256M (ACG→ATG) benRBenABC operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (203/168);  new base T (8/13);  total (211/181)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 393 total aligned reads displayed.

CCTGAGTGCCCCGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCGGCAGCA  >  NC_002947/3585475‑3585771
                                                                                                                                                     |                                                                                                                                                   
ccTGAGTGCCCCGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCAc                                                                                                                                                     <  2:7541612/150‑1 (MQ=255)
 cTGAGTGCCCCGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACg                                                                                                                                                    >  1:5497715/1‑150 (MQ=255)
 cTGAGTGCCCCGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACg                                                                                                                                                    >  1:6111756/1‑150 (MQ=255)
  tGAGTGCCCCGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGc                                                                                                                                                   <  1:5293652/150‑1 (MQ=255)
  tGAGTGCCCCGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGc                                                                                                                                                   >  1:1268077/1‑150 (MQ=255)
  tGAGTGCCCCGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGc                                                                                                                                                   >  1:10024/1‑150 (MQ=255)
        ccccGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCGTGATGCCCAAgt                                                                                                                                             <  1:3970202/150‑2 (MQ=255)
         cccGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCa                                                                                                                                            >  2:6141231/1‑150 (MQ=255)
          ccacAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCAt                                                                                                                                           >  2:2867822/4‑150 (MQ=255)
          ccGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCAt                                                                                                                                           <  1:483892/150‑1 (MQ=255)
           cGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCAtt                                                                                                                                          >  1:7490469/1‑150 (MQ=255)
             cAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTGTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTAc                                                                                                                                        <  1:5998858/150‑1 (MQ=255)
                    ggCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGcc                                                                                                                                 >  2:6434441/1‑150 (MQ=255)
                     gCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCa                                                                                                                                >  1:2896728/1‑150 (MQ=255)
                       tAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGc                                                                                                                              >  2:742674/1‑150 (MQ=255)
                            cgcATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGaaa                                                                                                                         >  2:1033376/1‑150 (MQ=255)
                            cgcATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGaaa                                                                                                                         >  1:3199471/1‑150 (MQ=255)
                              cATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACt                                                                                                                       >  2:3201387/1‑150 (MQ=255)
                                ttCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTgg                                                                                                                     >  2:5525024/1‑150 (MQ=255)
                                ttCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTgg                                                                                                                     >  2:5597228/1‑150 (MQ=255)
                                 tCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGa                                                                                                                    >  1:6473407/1‑150 (MQ=255)
                                 tCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGa                                                                                                                    >  2:5421567/1‑150 (MQ=255)
                                   ttGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAAc                                                                                                                  >  1:4581080/1‑150 (MQ=255)
                                    tGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACg                                                                                                                 <  2:5789088/150‑1 (MQ=255)
                                     gATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACgg                                                                                                                <  1:916907/150‑1 (MQ=255)
                                       ttACATCGAACGCAACCTGAAATTGGAATAGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGt                                                                                                              >  1:8009189/1‑150 (MQ=255)
                                          cATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTAGGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCa                                                                                                           >  1:7655147/1‑150 (MQ=255)
                                           aTCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCAt                                                                                                          <  2:656321/150‑1 (MQ=255)
                                           aTCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCCCAAACTGGAACGGGTGCAt                                                                                                          >  1:1117589/1‑150 (MQ=255)
                                              gAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCAtgcc                                                                                                       >  2:4232757/1‑150 (MQ=255)
                                               aaCGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCAtgcct                                                                                                      <  1:539486/150‑1 (MQ=255)
                                                 cGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCAtgcctgc                                                                                                    >  1:3341289/1‑150 (MQ=255)
                                                    aaCCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGcctgcctg                                                                                                 >  2:304785/1‑150 (MQ=255)
                                                      ccTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAg                                                                                               <  1:4489009/150‑1 (MQ=255)
                                                      ccTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAg                                                                                               >  1:3698383/1‑150 (MQ=255)
                                                       cTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGc                                                                                              >  2:2140310/1‑150 (MQ=255)
                                                       cTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGc                                                                                              >  1:1391448/1‑150 (MQ=255)
                                                       cTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGc                                                                                              >  2:7892061/1‑150 (MQ=255)
                                                       cTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGc                                                                                              <  2:2529696/150‑1 (MQ=255)
                                                       cTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGc                                                                                              >  2:8502806/1‑150 (MQ=255)
                                                       cTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGc                                                                                              >  1:1703898/1‑150 (MQ=255)
                                                         gAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGa                                                                                            >  1:2381017/1‑150 (MQ=255)
                                                           aaTTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGAcc                                                                                          <  2:4834906/150‑1 (MQ=255)
                                                            aTTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGAccc                                                                                         >  2:5659920/1‑150 (MQ=255)
                                                             ttGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCt                                                                                        >  2:8534227/1‑150 (MQ=255)
                                                              tGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTa                                                                                       >  1:7010438/1‑150 (MQ=255)
                                                                 aaTTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACtt                                                                                    <  1:6702877/150‑1 (MQ=255)
                                                                 aaTTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACtt                                                                                    <  2:6346890/150‑1 (MQ=255)
                                                                            gAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTgcgc                                                                         >  1:7136516/1‑150 (MQ=255)
                                                                             aaGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCa                                                                        >  2:6653697/1‑150 (MQ=255)
                                                                              aGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAg                                                                       <  2:8103726/150‑1 (MQ=255)
                                                                              aGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAg                                                                       >  2:1924603/1‑150 (MQ=255)
                                                                               gTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAgt                                                                      <  2:4420199/150‑1 (MQ=255)
                                                                                tGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAgtg                                                                     <  1:2237203/150‑1 (MQ=255)
                                                                                   tGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGa                                                                  >  1:4126947/1‑150 (MQ=255)
                                                                                     gCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACg                                                                >  2:912816/1‑150 (MQ=255)
                                                                                     gCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACg                                                                >  2:2199578/1‑150 (MQ=255)
                                                                                       agagCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGa                                                              >  1:2567718/1‑150 (MQ=255)
                                                                                        gagCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCGCTTGGGCGTGATGCCCaa                                                                                                                                               >  1:7354594/1‑68 (MQ=255)
                                                                                          gCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAAtt                                                           <  2:1680295/150‑1 (MQ=255)
                                                                                           cAGGCTTGCCTGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTg                                                          <  2:7735874/150‑1 (MQ=255)
                                                                                                 tGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTg                                                    <  1:2457966/150‑1 (MQ=255)
                                                                                                  gCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTgg                                                   <  1:3569519/150‑1 (MQ=255)
                                                                                                   cATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGa                                                  >  2:7052567/1‑150 (MQ=255)
                                                                                                   cATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGa                                                  >  1:8416820/1‑150 (MQ=255)
                                                                                                   cATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGa                                                  <  1:3622650/150‑1 (MQ=255)
                                                                                                   cATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGa                                                  <  2:4106170/150‑1 (MQ=255)
                                                                                                        gTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCAGTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACg                                             <  1:6528455/150‑1 (MQ=255)
                                                                                                         tttGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACgg                                            >  2:3934226/1‑150 (MQ=255)
                                                                                                            gCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGttt                                         <  2:3491756/150‑1 (MQ=255)
                                                                                                              gTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTcc                                       <  2:3368377/150‑1 (MQ=255)
                                                                                                                tCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCtt                                     >  1:2192337/1‑150 (MQ=255)
                                                                                                                 cGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTc                                    <  1:3404437/150‑1 (MQ=255)
                                                                                                                  gCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGACTTGGCCCTGGATTACGGTTTCCTTCa                                   >  2:4663590/1‑150 (MQ=255)
                                                                                                                  gCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCa                                   <  1:5661048/150‑1 (MQ=255)
                                                                                                                  gCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCa                                   <  1:7904336/150‑1 (MQ=255)
                                                                                                                   cTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCAt                                  <  2:6155699/150‑1 (MQ=255)
                                                                                                                    tGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATc                                 >  1:6596676/1‑150 (MQ=255)
                                                                                                                     gTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCt                                <  2:8712443/150‑1 (MQ=255)
                                                                                                                      tATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTg                               <  1:5258079/150‑1 (MQ=255)
                                                                                                                           gttgttCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCg                          <  1:5578356/150‑1 (MQ=255)
                                                                                                                              gttCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGtt                       >  1:5015572/1‑150 (MQ=255)
                                                                                                                                 cGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTc                    <  1:1483494/150‑1 (MQ=255)
                                                                                                                                  gACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTcc                   <  1:6274218/150‑1 (MQ=255)
                                                                                                                                  gACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTcc                   <  2:7320595/150‑1 (MQ=255)
                                                                                                                                     cAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCgag                >  1:3767125/1‑150 (MQ=255)
                                                                                                                                     cAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCgag                >  1:7217894/1‑150 (MQ=255)
                                                                                                                                     cAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCgag                >  2:7600918/1‑150 (MQ=255)
                                                                                                                                      aGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCgaga               <  2:1399193/150‑1 (MQ=255)
                                                                                                                                      aGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCgaga               >  2:8314509/1‑150 (MQ=255)
                                                                                                                                           ttGGGCGTGATGCCCAAGCATTACGTGCGCCAGCGGAAACGGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTAt          >  1:4196160/1‑150 (MQ=255)
                                                                                                                                           ttGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTAt          >  2:114742/1‑150 (MQ=255)
                                                                                                                                             gggCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCg        >  2:5122783/1‑150 (MQ=255)
                                                                                                                                              ggCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCgg       <  1:8565988/150‑1 (MQ=255)
                                                                                                                                              ggCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCgg       <  2:6838385/150‑1 (MQ=255)
                                                                                                                                                cATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGNTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCGgca     <  2:4576146/150‑1 (MQ=255)
                                                                                                                                                cATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCGgca     <  1:6853596/150‑1 (MQ=255)
                                                                                                                                                 aTCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCGgcag    >  1:5059652/1‑150 (MQ=255)
                                                                                                                                                  tCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCGgcagc   >  2:1813759/1‑150 (MQ=255)
                                                                                                                                                   cACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCGgcagca  >  1:5761293/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCTGAGTGCCCCGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCGGCAGCA  >  NC_002947/3585475‑3585771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑