Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,691,426 A→C 6.2% D281A (GAC→GCC)  PP_3251 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,691,4260AC 6.2% 1377.5 / 55.6 402D281A (GAC→GCC) PP_3251hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (175/202);  new base C (14/11);  total (189/213)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 405 total aligned reads displayed.

TGCCGCCAGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAG  >  NC_002947/3691277‑3691574
                                                                                                                                                     |                                                                                                                                                    
 gccgccAGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGAc                                                                                                                                                     >  2:5954105/1‑150 (MQ=255)
   cgccAGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCt                                                                                                                                                   >  2:1356760/1‑150 (MQ=255)
   cgccAGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCt                                                                                                                                                   >  1:8433042/1‑150 (MQ=255)
   cgccAGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCt                                                                                                                                                   >  1:4057121/1‑150 (MQ=255)
   cgccAGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCt                                                                                                                                                   <  1:3640161/150‑1 (MQ=255)
     ccAGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGc                                                                                                                                                 <  1:683892/150‑1 (MQ=255)
      atgCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCa                                                                                                                                                >  2:334121/3‑150 (MQ=255)
       aGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCaa                                                                                                                                               <  1:6690197/150‑1 (MQ=255)
       aGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCaa                                                                                                                                               <  1:3806523/150‑1 (MQ=255)
       aGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCaa                                                                                                                                               <  1:3331915/150‑1 (MQ=255)
             cACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                         >  2:8613146/1‑150 (MQ=255)
             cACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                         >  1:1001713/1‑150 (MQ=255)
             cACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                         <  2:6091233/150‑1 (MQ=255)
             cACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                         >  2:2261139/1‑150 (MQ=255)
               cgcccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGCCTTGCAGGTCGAACt                                                                                                                                       <  1:2816444/150‑1 (MQ=255)
               cgcccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGCCTTGCAGGTCGAACt                                                                                                                                       <  1:1353487/150‑1 (MQ=255)
                gcccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTg                                                                                                                                      >  1:7961516/1‑150 (MQ=255)
                  ccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGCCTTGCAGGTCGAACTggc                                                                                                                                    <  2:1904502/150‑3 (MQ=255)
                   cgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGttt                                                                                                                                   <  2:3014180/150‑1 (MQ=255)
                   cgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGttt                                                                                                                                   <  1:5653388/150‑1 (MQ=255)
                    gccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTg                                                                                                                                  <  2:5321705/150‑1 (MQ=255)
                    gccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTg                                                                                                                                  <  2:144787/150‑1 (MQ=255)
                          cAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGccc                                                                                                                            <  1:2345594/150‑1 (MQ=255)
                               cAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGaa                                                                                                                       <  2:5516761/150‑1 (MQ=255)
                               cAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGaa                                                                                                                       <  1:5390561/150‑1 (MQ=255)
                                  gcgcTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGc                                                                                                                    >  1:5924952/1‑150 (MQ=255)
                                  gcgcTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGc                                                                                                                    >  1:5726051/1‑150 (MQ=255)
                                        aaCCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCgg                                                                                                              <  1:948835/150‑1 (MQ=255)
                                                     ccGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAAt                                                                                                 >  1:4571038/1‑150 (MQ=255)
                                                        cgcATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGa                                                                                              <  1:751993/150‑1 (MQ=255)
                                                         gcATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGat                                                                                             <  2:6913957/150‑1 (MQ=255)
                                                         gcATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGat                                                                                             <  1:5068920/150‑1 (MQ=255)
                                                         gcATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGat                                                                                             <  2:6218411/150‑1 (MQ=255)
                                                         gcATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGat                                                                                             <  2:2410801/150‑1 (MQ=255)
                                                             gATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGatgcat                                                                                         <  1:5848563/150‑1 (MQ=255)
                                                             gATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTCGAACGCAAGCGGGTCTATGGCCAATTGatgcat                                                                                         >  1:7045907/1‑150 (MQ=255)
                                                               tCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGatgcatgc                                                                                       <  2:8455478/150‑1 (MQ=255)
                                                               tCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGatgcatgc                                                                                       >  2:1768068/1‑150 (MQ=255)
                                                               tCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGatgcatgc                                                                                       >  2:2865715/1‑150 (MQ=255)
                                                                  gCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGa                                                                                    <  2:3849671/150‑1 (MQ=255)
                                                                  gCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGa                                                                                    >  1:484281/1‑150 (MQ=255)
                                                                          tCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTgcc                                                                            <  1:3588569/150‑1 (MQ=255)
                                                                            tACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTgccgc                                                                          >  2:650668/1‑150 (MQ=255)
                                                                              cGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCt                                                                        <  1:7416931/150‑1 (MQ=255)
                                                                               gCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTg                                                                       >  1:662272/1‑150 (MQ=255)
                                                                               gCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTg                                                                       <  2:8247318/150‑1 (MQ=255)
                                                                               gCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTg                                                                       >  2:1125492/1‑150 (MQ=255)
                                                                                 cAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTgca                                                                     >  1:6786283/1‑150 (MQ=255)
                                                                                 cAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTgca                                                                     <  2:1631512/150‑1 (MQ=255)
                                                                                 cAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTgca                                                                     <  2:8120576/150‑1 (MQ=255)
                                                                                  aGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTgcag                                                                    >  2:4981564/1‑150 (MQ=255)
                                                                                    ggCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTgcagca                                                                  <  1:4120212/150‑1 (MQ=255)
                                                                                      cgcgcTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCagcagc                                                                <  2:4848285/150‑1 (MQ=255)
                                                                                      cgcgcTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCagcagc                                                                <  1:4402757/150‑1 (MQ=255)
                                                                                       gcgcTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAgcg                                                               >  2:4144556/1‑150 (MQ=255)
                                                                                         gcTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCa                                                             >  2:4691274/1‑150 (MQ=255)
                                                                                            tAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGa                                                          <  2:4445031/150‑1 (MQ=255)
                                                                                             aGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGat                                                         <  2:4574771/150‑1 (MQ=255)
                                                                                             aGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGat                                                         <  2:4372510/150‑1 (MQ=255)
                                                                                               gCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGatca                                                       >  1:2760018/1‑150 (MQ=255)
                                                                                               gCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTCGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGatca                                                       <  2:7045907/150‑1 (MQ=255)
                                                                                                 tACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGatcaat                                                     <  2:7383486/150‑1 (MQ=255)
                                                                                                 tACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGatcaat                                                     <  2:2651692/150‑1 (MQ=255)
                                                                                                  aCCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGatcaatc                                                    <  2:5078804/150‑1 (MQ=255)
                                                                                                    cGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGAtcaatcaa                                                  <  1:118284/150‑1 (MQ=255)
                                                                                                    cGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGAtcaatcaa                                                  >  1:8705358/1‑150 (MQ=255)
                                                                                                     gCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATcaatcaat                                                 <  1:6341956/150‑1 (MQ=255)
                                                                                                     gCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATcaatcaat                                                 <  1:3392624/150‑1 (MQ=255)
                                                                                                     gCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATcaatcaat                                                 <  2:7302232/150‑1 (MQ=255)
                                                                                                      ccTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAAtg                                                >  2:3760203/1‑150 (MQ=255)
                                                                                                          aaTGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCgg                                                                                                              <  1:6109802/84‑1 (MQ=255)
                                                                                                            tGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAAtggctggc                                          <  2:324592/150‑1 (MQ=255)
                                                                                                             gACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGatca                                                       <  1:6957012/136‑1 (MQ=255)
                                                                                                                   ggTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAAccc                                   >  1:8194030/1‑150 (MQ=255)
                                                                                                                      aTCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGgc                                <  1:2142479/150‑1 (MQ=255)
                                                                                                                        cgccacgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGgcgc                              <  1:1698102/150‑1 (MQ=255)
                                                                                                                         gccacgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGcc                             >  1:256466/1‑150 (MQ=255)
                                                                                                                          ccacgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCa                            >  1:3707549/1‑150 (MQ=255)
                                                                                                                          ccacgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCa                            >  1:6486747/1‑150 (MQ=255)
                                                                                                                            acgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATg                          >  1:7812936/1‑150 (MQ=255)
                                                                                                                             cgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGc                         <  2:680607/150‑1 (MQ=255)
                                                                                                                             cgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGc                         <  2:5028134/150‑1 (MQ=255)
                                                                                                                             cgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGc                         <  2:5853865/150‑1 (MQ=255)
                                                                                                                                caGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCg                      >  1:3139967/1‑150 (MQ=255)
                                                                                                                                 aGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGc                     >  1:5398617/1‑150 (MQ=255)
                                                                                                                                 aGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGc                     >  2:7861212/1‑150 (MQ=255)
                                                                                                                                    ccTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCAtg                  >  2:5174441/1‑149 (MQ=255)
                                                                                                                                        aTCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGtt              <  1:2460576/150‑1 (MQ=255)
                                                                                                                                        aTCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGtt              <  2:1561770/150‑1 (MQ=255)
                                                                                                                                        aTCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGtt              >  1:3939689/1‑150 (MQ=255)
                                                                                                                                        aTCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGtt              >  1:1865939/1‑150 (MQ=255)
                                                                                                                                         tCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGTTGGCCGAACCCGGCGCCATGCCCGCATTCGTTg             <  1:1505285/150‑1 (MQ=255)
                                                                                                                                         tCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTg             >  1:5190096/1‑150 (MQ=255)
                                                                                                                                          caaacAGTTCGCCTTGCAGGTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGc            >  1:2919746/5‑150 (MQ=255)
                                                                                                                                           aaacAGTTCGCCTTGCAGGTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCAGAACCCGGCGCCATGCCCGAATACGTTGcc           >  1:7158375/4‑150 (MQ=255)
                                                                                                                                            gCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCg          >  2:315361/1‑150 (MQ=255)
                                                                                                                                            gCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGAGTGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGTCCGAACCCGGCGCCATGCCCGCATTCGTTGCCg          >  1:2011500/1‑150 (MQ=255)
                                                                                                                                             ccAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGc         >  2:6608017/1‑150 (MQ=255)
                                                                                                                                             acAGTTCGCCTTGCAGGTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGc         >  2:4008853/2‑150 (MQ=255)
                                                                                                                                             acAGTTCGCCTTGCAGGTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGc         >  2:3560831/2‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCCGCCAGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAG  >  NC_002947/3691277‑3691574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑