BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,706,977	C→A	5.2%	L139M (CTG→ATG) ‡	PP_3267 →	Clp protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,706,977	0	C	A	5.2%	1315.3 / 39.7	387	L139M (CTG→ATG) ‡	PP_3267	Clp protease
Reads supporting (aligned to +/- strand): ref base C (166/201); new base A (12/8); total (178/209)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 393 total aligned reads displayed.

GGCAATATCCTCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATCGCG  >  NC_002947/3706830‑3707126

  cAATATCCTCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTa                                                                                                                                                   <  1:6044623/150‑1 (MQ=255)

   aaTATCCTCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTAt                                                                                                                                                  <  2:3200096/150‑1 (MQ=255)

   aaTATCCTCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTAt                                                                                                                                                  >  1:4585305/1‑150 (MQ=255)

   aaTATCCTCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTAt                                                                                                                                                  >  1:8271296/1‑150 (MQ=255)

     tatCCTCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATgc                                                                                                                                                >  2:2915646/1‑150 (MQ=255)

       tCCTCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATgcgc                                                                                                                                              >  1:8808972/1‑150 (MQ=255)

        ccTCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCa                                                                                                                                             >  2:5518853/1‑150 (MQ=255)

        ccTCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCCGGATTACGACCTGCGCCTGTATGCGCa                                                                                                                                             >  2:2024452/1‑150 (MQ=255)

          tctTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGa                                                                                                                                           <  1:6305012/150‑1 (MQ=255)

          tctTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGa                                                                                                                                           >  1:5011622/1‑150 (MQ=255)

            ttCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCATACAGGCGCAGGTc                                                                                                                                         <  1:3952750/150‑1 (MQ=255)

            ttCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCATACAGGCGCAGGTc                                                                                                                                         <  2:8700629/150‑1 (MQ=255)

               cgggCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTGTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTg                                                                                                                                      <  2:70282/147‑1 (MQ=255)

               cTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTg                                                                                                                                      >  2:8031359/1‑150 (MQ=255)

               cTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTg                                                                                                                                      <  2:1317506/150‑1 (MQ=255)

                ttGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGATCTGCGCATACAGGCGCAGGTCGtaa                                                                                                                                     <  1:1994653/150‑3 (MQ=255)

                  gCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGATCTGCGCATACAGGCGCAGGTCGtaatc                                                                                                                                   <  1:8534872/150‑5 (MQ=255)

                   ccGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCgg                                                                                                                                  >  2:4537041/1‑150 (MQ=255)

                    cGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGa                                                                                                                                 >  2:2582663/1‑150 (MQ=255)

                      gagaGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAgc                                                                                                                               <  1:5834170/150‑1 (MQ=255)

                      gagaGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAgc                                                                                                                               <  2:4560551/150‑1 (MQ=255)

                             aCGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAgcgcctttc                                                                                                                        <  1:2483859/150‑6 (MQ=255)

                             aCGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGaa                                                                                                                        >  1:7816426/1‑150 (MQ=255)

                               gCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAg                                                                                                                      <  1:3209880/150‑1 (MQ=255)

                               gCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAg                                                                                                                      <  1:3091145/150‑1 (MQ=255)

                                cACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAgg                                                                                                                     >  2:8782212/1‑150 (MQ=255)

                                cACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAgg                                                                                                                     >  2:77087/1‑150 (MQ=255)

                                 aCGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGgc                                                                                                                    >  1:43133/1‑150 (MQ=255)

                                 aCGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGgc                                                                                                                    >  1:6618596/1‑150 (MQ=255)

                                 aCGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGgc                                                                                                                    >  2:6070611/1‑150 (MQ=255)

                                 aCGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGgc                                                                                                                    <  2:6691941/150‑1 (MQ=255)

                                     cTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCgg                                                                                                                >  2:7473031/1‑150 (MQ=255)

                                      ttGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGc                                                                                                               >  2:451002/1‑150 (MQ=255)

                                                aGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTg                                                                                                     >  1:7170326/1‑150 (MQ=255)

                                                aGCAAGTTTCTTGTCCCCCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTg                                                                                                     <  2:6163771/150‑1 (MQ=255)

                                                 gCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTgg                                                                                                    >  2:2405729/1‑150 (MQ=255)

                                                  cAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGa                                                                                                   <  2:4975562/150‑1 (MQ=255)

                                                  cAAGTTTCTTTGCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGa                                                                                                   <  2:7302460/150‑1 (MQ=255)

                                                         cTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACcg                                                                                            >  1:7994063/1‑150 (MQ=255)

                                                          ttttCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACcgc                                                                                           >  2:3319033/1‑150 (MQ=255)

                                                           tttCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACcgcg                                                                                          >  2:8533135/1‑150 (MQ=255)

                                                           tttCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTCGGATGTACcgcg                                                                                          >  1:2109860/1‑150 (MQ=255)

                                                                aCCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATc                                                                                     >  2:6466322/1‑150 (MQ=255)

                                                                aCCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATc                                                                                     <  2:4372482/150‑1 (MQ=255)

                                                                aCCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATc                                                                                     <  1:8147441/150‑1 (MQ=255)

                                                                  ccGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTa                                                                                   >  1:6759970/1‑150 (MQ=255)

                                                                   cGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTaa                                                                                  <  1:7961463/150‑1 (MQ=255)

                                                                     tttCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATg                                                                                >  1:5472338/1‑150 (MQ=255)

                                                                     tttCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATg                                                                                <  1:2284374/150‑1 (MQ=255)

                                                                       tCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAg                                                                                                           <  1:8047556/121‑1 (MQ=255)

                                                                       tCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGc                                                                              <  1:451002/150‑1 (MQ=255)

                                                                       tCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGc                                                                              <  2:7557688/150‑1 (MQ=255)

                                                                         aTTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCt                                                                            >  2:1547382/1‑150 (MQ=255)

                                                                         aTTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCt                                                                            >  2:3952173/1‑150 (MQ=255)

                                                                          ttGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGGTATCTAATGGCCTa                                                                           >  1:1001759/1‑150 (MQ=255)

                                                                               ccTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAGAGGCGCGGCCGAGGTTTTg                                                                                                     <  2:2785173/119‑1 (MQ=255)

                                                                               ccTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCaa                                                                      <  1:1334812/150‑1 (MQ=255)

                                                                               ccTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCaa                                                                      <  2:3863653/150‑1 (MQ=255)

                                                                               ccTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCaa                                                                      <  1:6881800/150‑1 (MQ=255)

                                                                                 ttGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAgg                                                                    >  1:7338319/1‑150 (MQ=255)

                                                                                        gTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTgg                                                             <  2:3471268/150‑1 (MQ=255)

                                                                                         ttCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGgc                                                            >  1:3627740/1‑150 (MQ=255)

                                                                                             gTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGcgccg                                                        >  1:7458912/1‑150 (MQ=255)

                                                                                              tGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGcgccgc                                                       >  2:6288203/1‑150 (MQ=255)

                                                                                              tGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGcgccgc                                                       <  1:1069179/150‑1 (MQ=255)

                                                                                                gACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCgg                                                     <  1:1785673/150‑1 (MQ=255)

                                                                                                gACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCgg                                                     >  2:8327304/1‑150 (MQ=255)

                                                                                                gACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCgg                                                     <  1:3713015/150‑1 (MQ=255)

                                                                                                gACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCgg                                                     <  2:480579/150‑1 (MQ=255)

                                                                                                  ccATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGa                                                   <  1:2886719/150‑1 (MQ=255)

                                                                                                     ttCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGgc                                                >  1:4094872/1‑150 (MQ=255)

                                                                                                        gcgGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCt                                             <  2:2129762/150‑1 (MQ=255)

                                                                                                        gcgGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCt                                             <  1:1428999/150‑1 (MQ=255)

                                                                                                         cgGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTg                                            <  1:5518853/150‑1 (MQ=255)

                                                                                                         cgGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTg                                            <  2:7400034/150‑1 (MQ=255)

                                                                                                                ccGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACg                                     <  2:4164559/150‑1 (MQ=255)

                                                                                                                 cGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGGAAACgg                                    <  1:3445163/150‑1 (MQ=255)

                                                                                                                   aGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGga                                  <  2:7913229/150‑1 (MQ=255)

                                                                                                                      aTGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACg                                     <  2:8438886/144‑1 (MQ=255)

                                                                                                                        gCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATc                             <  1:7110260/150‑1 (MQ=255)

                                                                                                                         cGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATcc                            >  2:8185721/1‑150 (MQ=255)

                                                                                                                          gATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCa                           <  2:5550456/150‑1 (MQ=255)

                                                                                                                          gATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCa                           >  1:5655252/1‑150 (MQ=255)

                                                                                                                               gCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGaaa                      <  2:4628607/150‑1 (MQ=255)

                                                                                                                                 cTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATc                    >  1:5868308/1‑150 (MQ=255)

                                                                                                                                 cTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATc                    <  2:5269962/150‑1 (MQ=255)

                                                                                                                                  tccgccaCGATCTGCGCATACAGGCGCAGGTCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcg                   >  1:3278313/7‑150 (MQ=255)

                                                                                                                                  tccgccaCGATCTGCGCATACAGGCGCAGGTCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcg                   >  1:7721373/7‑150 (MQ=255)

                                                                                                                                   ggATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcga                  >  1:5276059/1‑150 (MQ=255)

                                                                                                                                    gATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGTTCCATGAAATcgac                 <  2:6879991/150‑1 (MQ=255)

                                                                                                                                    gATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgac                 <  2:1813739/150‑1 (MQ=255)

                                                                                                                                     aTTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgacg                >  2:1038609/1‑150 (MQ=255)

                                                                                                                                      ttACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgacga               <  2:7429447/150‑1 (MQ=255)

                                                                                                                                      ttACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgacga               <  1:7569374/150‑1 (MQ=255)

                                                                                                                                      ccaCGATCTGCGCATACAGGCGCAGGTCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGTTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgacga               >  1:2166461/3‑150 (MQ=255)

                                                                                                                                        aCGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACgaga             <  1:8682637/150‑1 (MQ=255)

                                                                                                                                          gACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATg           >  1:336711/1‑150 (MQ=255)

                                                                                                                                             cTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCg        >  1:2699069/1‑150 (MQ=255)

                                                                                                                                                cgcCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATc     <  1:4188057/150‑1 (MQ=255)

                                                                                                                                                 gcCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATcg    >  2:193296/1‑150 (MQ=255)

GGCAATATCCTCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATCGCG  >  NC_002947/3706830‑3707126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`