BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,143,578	C→A	5.8%	G28G (GGC→GGA)	PP_3643 →	oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,143,578	0	C	A	5.8%	983.5 / 34.4	294	G28G (GGC→GGA)	PP_3643	oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (148/128); new base A (8/9); total (156/138)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 299 total aligned reads displayed.

GTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAACAGCA  >  NC_002947/4143430‑4143727

gTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTCCTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGcg                                                                                                                                                      >  2:7202025/1‑149 (MQ=255)

 tataCCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTc                                                                                                                                                     >  2:2282931/1‑150 (MQ=255)

  ataCCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCg                                                                                                                                                    <  2:726503/150‑1 (MQ=255)

  agaCCATCAGACCCCTCAACGATACAAACCGCGGACCCCCGCAGCCCTTCCGAGGTACTCCCGATGATCGACGCAACGGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCg                                                                                                                                                    <  1:1940552/148‑1 (MQ=255)

   taCCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCgg                                                                                                                                                   <  1:1619570/150‑1 (MQ=255)

     ccATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGgtc                                                                                                                                                 <  1:8087473/150‑1 (MQ=255)

          aGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCgg                                                                                                                                            >  2:5789586/1‑150 (MQ=255)

           gACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGc                                                                                                                                           >  2:1454701/1‑150 (MQ=255)

            acacATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGcc                                                                                                                                          >  2:1536513/1‑150 (MQ=255)

             cacaTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGTCGTCGTCCt                                                                                                                                         <  2:5213583/150‑1 (MQ=255)

             cacaTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCt                                                                                                                                         <  1:2101867/150‑1 (MQ=255)

                aTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGAc                                                                                                                                      <  2:8685391/150‑1 (MQ=255)

                aTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGAc                                                                                                                                      >  2:1114799/1‑150 (MQ=255)

                aTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGAc                                                                                                                                      >  1:2423196/1‑150 (MQ=255)

                 tCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGTCGTCGTCCTgggt                                                                                                                                     <  2:571792/150‑4 (MQ=255)

                   aaCGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGc                                                                                                                                   <  1:3062187/150‑1 (MQ=255)

                    aCGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGcc                                                                                                                                  >  2:8067115/1‑150 (MQ=255)

                    aCGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGcc                                                                                                                                  <  1:4389454/150‑1 (MQ=255)

                     cGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCa                                                                                                                                 <  2:8775946/150‑1 (MQ=255)

                       aTAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAgc                                                                                                                               <  1:962463/150‑1 (MQ=255)

                       aTAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAgc                                                                                                                               >  2:2070870/1‑150 (MQ=255)

                        tAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAgcg                                                                                                                              >  2:3079259/1‑150 (MQ=255)

                            aaCCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCtt                                                                                                                          <  2:4080485/150‑1 (MQ=255)

                            aaCCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCtt                                                                                                                          <  2:7576658/150‑1 (MQ=255)

                               cgcgGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTtctc                                                                                                                       >  2:6442525/1‑150 (MQ=255)

                               cgcgGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTtctc                                                                                                                       <  1:2797502/150‑1 (MQ=255)

                                gcgGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTctcc                                                                                                                      <  1:5821026/150‑1 (MQ=255)

                                   gACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCtcctcc                                                                                                                   <  1:1300228/150‑1 (MQ=255)

                                   gACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCtcctcc                                                                                                                   <  2:6036040/150‑1 (MQ=255)

                                      caccGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGTCCGCCAGCGCCTTCTCCTCCCTg                                                                                                                >  2:6495083/1‑150 (MQ=255)

                                      caccGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTg                                                                                                                >  2:6335589/1‑150 (MQ=255)

                                       accGCAGCCCTTCTGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCGGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACTACGACGGGTGGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGt                                                                                                               <  1:6189264/150‑1 (MQ=255)

                                         cGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCg                                                                                                             >  2:5133042/1‑150 (MQ=255)

                                            aGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATg                                                                                                          <  1:5045925/150‑1 (MQ=255)

                                              cccTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGa                                                                                                        >  1:6504156/1‑150 (MQ=255)

                                               ccTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGGTGGAc                                                                                                       >  1:1897129/1‑150 (MQ=255)

                                                cTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTACTCCTCCCTGTCGATGGAcc                                                                                                      >  2:1641963/1‑150 (MQ=255)

                                                          aCTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCGGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGt                                                                                            <  2:362343/150‑1 (MQ=255)

                                                            tCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGc                                                                                          >  1:3664962/1‑150 (MQ=255)

                                                             cccGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCt                                                                                         <  1:1722108/150‑1 (MQ=255)

                                                              ccGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTg                                                                                        >  1:7653256/1‑150 (MQ=255)

                                                              ccGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTg                                                                                        >  2:5590564/1‑150 (MQ=255)

                                                              ccGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTg                                                                                        >  1:8753603/1‑150 (MQ=255)

                                                                   gatCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTg                                                                                   <  2:2005467/150‑1 (MQ=255)

                                                                      cGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGccc                                                                                <  1:8681746/150‑1 (MQ=255)

                                                                       gACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGcccc                                                                               <  2:4260735/150‑1 (MQ=255)

                                                                         cGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCaa                                                                             <  2:4726373/150‑1 (MQ=255)

                                                                          gCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAAc                                                                            <  1:5485200/150‑1 (MQ=255)

                                                                          gCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAAc                                                                            <  2:3723545/150‑1 (MQ=255)

                                                                          gCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAAc                                                                            <  1:7998616/150‑1 (MQ=255)

                                                                          gCAACTGTCTACAAGAACGTTCGGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAAc                                                                            <  1:2462642/150‑1 (MQ=255)

                                                                               tGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTacac                                                                       <  1:4267123/150‑1 (MQ=255)

                                                                               tGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTacac                                                                       >  1:5044200/1‑150 (MQ=255)

                                                                               tGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTacac                                                                       >  2:7686293/1‑150 (MQ=255)

                                                                                   tACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTcc                                                                   >  1:6078427/1‑150 (MQ=255)

                                                                                    aCAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCg                                                                  >  2:2968132/1‑150 (MQ=255)

                                                                                     cAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGa                                                                 >  2:4598260/1‑150 (MQ=255)

                                                                                      aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGAc                                                                >  1:2836722/1‑150 (MQ=255)

                                                                                      aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGAc                                                                <  1:4607231/150‑1 (MQ=255)

                                                                                      aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGAc                                                                >  2:1195479/1‑150 (MQ=255)

                                                                                      aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGAc                                                                <  1:1091451/150‑1 (MQ=255)

                                                                                       aGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCGACTACACCTCCGACt                                                               >  1:5301441/1‑150 (MQ=255)

                                                                                            gTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTAc                                                          >  1:677620/1‑150 (MQ=255)

                                                                                            gTTCTGGGCTCCGTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTAc                                                          <  2:7434112/150‑1 (MQ=255)

                                                                                             ttCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTAcc                                                         >  2:3497683/1‑150 (MQ=255)

                                                                                              tCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTAccc                                                        >  1:853643/1‑150 (MQ=255)

                                                                                              tCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTAccc                                                        <  2:3210146/150‑1 (MQ=255)

                                                                                                  ggCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTa                                                    <  2:5015808/150‑1 (MQ=255)

                                                                                                       cTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCCGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCCACTACACCTCCGACTCCTCCCCGGTCCTGAt                                               >  2:8458672/1‑150 (MQ=255)

                                                                                                           ccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAg                                           >  1:327935/1‑150 (MQ=255)

                                                                                                             gTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCa                                         <  1:7867196/150‑1 (MQ=255)

                                                                                                               ccGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGa                                       <  2:2640568/150‑1 (MQ=255)

                                                                                                                 ggCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAg                                     >  2:7394334/1‑150 (MQ=255)

                                                                                                                 ggCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAg                                     >  2:7903202/1‑150 (MQ=255)

                                                                                                                     ttACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCt                                 >  1:4966250/1‑150 (MQ=255)

                                                                                                                        ccGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTg                              <  1:1536513/150‑1 (MQ=255)

                                                                                                                         cGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGc                             <  1:4570419/150‑1 (MQ=255)

                                                                                                                          gTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGcc                            >  2:6797574/1‑150 (MQ=255)

                                                                                                                           tcatcaCCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCa                           <  1:3026033/150‑1 (MQ=255)

                                                                                                                            catcaCCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCAt                          <  1:4094391/150‑1 (MQ=255)

                                                                                                                             atcaCCACCCAGGACGACGACGGCTCGGTAGTCGGCCTGACCGACAGAGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTATGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGAAGAAGCTCTTTgaaatt                         >  1:4733632/1‑145 (MQ=255)

                                                                                                                              tcaCCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTc                        <  1:1010804/150‑1 (MQ=255)

                                                                                                                              tcaCCACCCAGGACGACGACGGCTCGGTCGTCGGCCAGACCGCCAGAGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGAGCTTTGCCATTc                        >  2:4373770/1‑150 (MQ=255)

                                                                                                                               caccacCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCa                       >  1:7063956/1‑150 (MQ=255)

                                                                                                                                accaccCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCAc                      >  2:3417195/1‑150 (MQ=255)

                                                                                                                                 gcggtcAGGCCGACGACCGAGCCGTCGTCGTCCTGGCCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCAcc                     >  2:3816148/6‑150 (MQ=37)

                                                                                                                                  caccCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACct                    >  1:3974346/1‑150 (MQ=255)

                                                                                                                                   accCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctg                   <  1:7918814/150‑1 (MQ=255)

                                                                                                                                     ccAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGTCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctgct                 >  1:3588707/1‑150 (MQ=255)

                                                                                                                                     ccAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctgct                 >  1:2265299/1‑150 (MQ=255)

                                                                                                                                     ccAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctgct                 >  2:3944557/1‑150 (MQ=255)

                                                                                                                                       aGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGt               >  2:2264795/1‑150 (MQ=255)

                                                                                                                                       aGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGt               >  1:2892662/1‑150 (MQ=255)

                                                                                                                                         gacgacgacGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTcc             >  2:2393546/1‑150 (MQ=255)

                                                                                                                                            gacgacgGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGt          >  1:8719069/1‑150 (MQ=255)

                                                                                                                                            gacgacgGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGt          <  1:8067115/150‑1 (MQ=255)

                                                                                                                                             acgacgGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTg         >  2:5384896/1‑150 (MQ=255)

                                                                                                                                                  ggCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAAcag    >  1:7398930/1‑150 (MQ=255)

                                                                                                                                                    cTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAAcagca  >  2:6937962/1‑150 (MQ=255)

                                                                                                                                                    cTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAAcagca  >  2:2495473/1‑150 (MQ=255)

GTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAACAGCA  >  NC_002947/4143430‑4143727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`