Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,245,240 C→A 5.1% A15S (GCT→TCT)  PP_3717 ← LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,245,2400CA 5.1% 1323.8 / 40.8 388A15S (GCT→TCT) PP_3717LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (185/183);  new base A (11/9);  total (196/192)
Fisher's exact test for biased strand distribution p-value = 8.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 396 total aligned reads displayed.

GCCGATGGCAGCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGTGATGATCAACGG  >  NC_002947/4245091‑4245389
                                                                                                                                                     |                                                                                                                                                     
 ccGATGGCAGCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGcc                                                                                                                                                      >  1:7795975/1‑150 (MQ=255)
  cGATGGCAGCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCt                                                                                                                                                     >  2:2285532/1‑150 (MQ=255)
  cGATGGCAGCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCt                                                                                                                                                     >  2:1964425/1‑150 (MQ=255)
   gATGGCAGCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTg                                                                                                                                                    <  2:4398999/150‑1 (MQ=255)
     tgccatcGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGcc                                                                                                                                                  >  1:992385/7‑150 (MQ=255)
     tGGCAGCGGCCACATACTCCCGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGcc                                                                                                                                                  <  1:4379013/150‑1 (MQ=255)
     tGGCAGCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGcc                                                                                                                                                  <  1:1348525/150‑1 (MQ=255)
     tGGCAGCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGcc                                                                                                                                                  <  2:7638825/150‑1 (MQ=255)
      ggCAGCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCa                                                                                                                                                 >  1:4756805/1‑150 (MQ=255)
         aGCGGCCACATCCTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGc                                                                                                                                              <  2:5530689/150‑1 (MQ=255)
          gCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCTGGCAGGCTAGACTGCCGGGCa                                                                                                                                             <  2:6776489/150‑1 (MQ=255)
              ccACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGcc                                                                                                                                         >  2:5741612/1‑150 (MQ=255)
              ccACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGcc                                                                                                                                         >  2:1318817/1‑150 (MQ=255)
               cacaTACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGccc                                                                                                                                        >  1:1391409/1‑150 (MQ=255)
                acaTACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCcg                                                                                                                                       >  1:2867599/1‑150 (MQ=255)
                acaTACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCcg                                                                                                                                       >  2:105278/1‑150 (MQ=255)
                acaTACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCcg                                                                                                                                       >  1:2672192/1‑150 (MQ=255)
                  aTACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCcgcg                                                                                                                                     >  1:6086227/1‑150 (MQ=255)
                  aTACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCcgcg                                                                                                                                     >  1:195549/1‑150 (MQ=255)
                      tCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGcaca                                                                                                                                 >  2:1177352/1‑150 (MQ=255)
                      tCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGcaca                                                                                                                                 >  1:5854697/1‑150 (MQ=255)
                        cAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCACCGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGCGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGc                                                                                                                               >  1:4123403/1‑150 (MQ=255)
                         cgcgcCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACGGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGcc                                                                                                                              <  2:7370892/149‑1 (MQ=255)
                         aGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGcc                                                                                                                              >  2:4902172/1‑150 (MQ=255)
                         aGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGcc                                                                                                                              >  1:1567475/1‑150 (MQ=255)
                             ccTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCttt                                                                                                                          <  1:5416369/150‑1 (MQ=255)
                               tCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTgc                                                                                                                        >  1:6916192/1‑150 (MQ=255)
                               tCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTgc                                                                                                                        <  2:8556085/150‑1 (MQ=255)
                                 gggggTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGGCTAGACTGCCggg                                                                                                                                               <  1:8338078/125‑1 (MQ=255)
                                 gggggTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCAGTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGGCTAGACTGCCggg                                                                                                                                               >  2:8338078/1‑125 (MQ=255)
                                  ggggTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGACAGGCACGCCCGCGCACAGCCCTTTGCGcc                                                                                                                     <  1:5076985/150‑1 (MQ=255)
                                   gggTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCa                                                                                                                    >  2:6274953/1‑150 (MQ=255)
                                     gTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAgg                                                                                                                  >  1:6014596/1‑150 (MQ=255)
                                      tttCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGt                                                                                                                 >  2:6187430/1‑150 (MQ=255)
                                      tttCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGt                                                                                                                 >  1:5311464/1‑150 (MQ=255)
                                         cACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGGATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACCCCCGCGCACAGCCCATTGCGCCCGGTTCa                                                                                                              >  1:6264175/1‑150 (MQ=255)
                                          aCCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCATTGCGCCAGGTTCAt                                                                                                             >  1:133145/1‑150 (MQ=255)
                                                  ccAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTg                                                                                                     <  2:8452940/150‑1 (MQ=255)
                                                      ggCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTAc                                                                                                 <  2:3358157/150‑1 (MQ=255)
                                                       gcgcgcTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACa                                                                                                <  1:6499874/150‑1 (MQ=255)
                                                           gcTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGcc                                                                                            >  1:3092494/1‑150 (MQ=255)
                                                             tGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCaa                                                                                          >  2:586490/1‑150 (MQ=255)
                                                             tGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGACAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCaa                                                                                          >  1:5263608/1‑150 (MQ=255)
                                                                aaCGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAgcg                                                                                       >  1:6556095/1‑150 (MQ=255)
                                                                aaCGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAgcg                                                                                       <  1:1710500/150‑1 (MQ=255)
                                                                   gCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAggccga                                                                                    <  1:4832715/150‑6 (MQ=255)
                                                                   gCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCg                                                                                    <  2:1665405/150‑1 (MQ=255)
                                                                     cACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTg                                                                                  >  2:5158902/1‑150 (MQ=255)
                                                                      ccGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGa                                                                                 <  2:3192001/149‑1 (MQ=255)
                                                                      aCGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGa                                                                                 <  2:4304124/150‑1 (MQ=255)
                                                                      aCGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGa                                                                                 <  2:6973306/150‑1 (MQ=255)
                                                                        gTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGt                                                                               >  2:8798225/1‑150 (MQ=255)
                                                                            cACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCAc                                                                           <  2:1913192/150‑1 (MQ=255)
                                                                             aCTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCAcg                                                                          >  2:6383408/1‑150 (MQ=255)
                                                                               tgctgcAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCAcgcg                                                                        >  1:4565774/1‑150 (MQ=255)
                                                                                gctgcAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCAcgcgc                                                                       <  1:3620789/150‑1 (MQ=255)
                                                                                 ctgcAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCAcgcgcg                                                                      <  1:3756960/150‑1 (MQ=255)
                                                                                 ctgcAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCAcgcgcg                                                                      <  1:5594173/150‑1 (MQ=255)
                                                                                   gcAGCGCGCCCATGGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGt                                                                    >  1:6623727/1‑150 (MQ=255)
                                                                                       cgcgcCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCa                                                                <  1:7191622/150‑1 (MQ=255)
                                                                                        gcgcCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCaa                                                               >  2:5254056/1‑150 (MQ=255)
                                                                                        gcgcCCAATGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGAACAGGCCAAGCGCCGTGAGTTCACGCGCGGtctcca                                                               <  2:3742973/150‑3 (MQ=255)
                                                                                          gcCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAatg                                                             >  1:8198210/1‑150 (MQ=255)
                                                                                          gcCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAatg                                                             >  2:3456618/1‑150 (MQ=255)
                                                                                            ccATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAatgat                                                           <  2:1462600/150‑1 (MQ=255)
                                                                                            ccATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAatgat                                                           >  2:7727197/1‑150 (MQ=255)
                                                                                             cATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATc                                                                                                            >  2:539298/1‑100 (MQ=255)
                                                                                                 gtgAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACt                                                      <  1:8659647/150‑1 (MQ=255)
                                                                                                  tgAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTg                                                     >  1:4989598/1‑150 (MQ=255)
                                                                                                  tgAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTg                                                     >  1:336172/1‑150 (MQ=255)
                                                                                                     gggTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGgcg                                                  >  2:784430/1‑150 (MQ=255)
                                                                                                      ggTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGgcgc                                                 >  2:5971045/1‑150 (MQ=255)
                                                                                                         aaGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAgg                                              <  2:7094583/150‑1 (MQ=255)
                                                                                                         aaGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAgg                                              <  1:2353511/150‑1 (MQ=255)
                                                                                                         aaGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAgg                                              <  2:1801000/150‑1 (MQ=255)
                                                                                                             ggggTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGtct                                          <  1:8583398/150‑1 (MQ=255)
                                                                                                                gTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACCGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTcga                                       >  2:8693501/1‑150 (MQ=255)
                                                                                                                gTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTcga                                       <  1:7398621/150‑1 (MQ=255)
                                                                                                                gTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTcga                                       <  1:464762/150‑1 (MQ=255)
                                                                                                                    cGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGAt                                   <  2:801859/150‑1 (MQ=255)
                                                                                                                    cGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGAt                                   >  1:6147366/1‑150 (MQ=255)
                                                                                                                             ccTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCAc                          >  1:2588189/1‑150 (MQ=255)
                                                                                                                             ccTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCAc                          <  2:1254665/150‑1 (MQ=255)
                                                                                                                             ccTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCAc                          <  1:3900229/150‑1 (MQ=255)
                                                                                                                                  gTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTgcg                     >  2:8163267/1‑150 (MQ=255)
                                                                                                                                     aTGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCag                  >  1:8767701/1‑149 (MQ=255)
                                                                                                                                      tGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATg                 <  2:4123403/150‑1 (MQ=255)
                                                                                                                                       gCCTGGCAGGCTAGACTGCCGGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATgg                >  2:2502280/1‑150 (MQ=255)
                                                                                                                                       gCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATgg                <  1:1000841/150‑1 (MQ=255)
                                                                                                                                       gCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATgg                <  2:2127208/150‑1 (MQ=255)
                                                                                                                                       gCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATgg                <  1:1148574/150‑1 (MQ=255)
                                                                                                                                         ctggCAGGCTAGACTGCCGGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGt              >  2:2589121/3‑150 (MQ=255)
                                                                                                                                         ccGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGt              <  2:6086227/150‑1 (MQ=255)
                                                                                                                                            gCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGtgat           >  1:6760546/1‑150 (MQ=255)
                                                                                                                                              aGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGtgatga         >  1:1838372/1‑150 (MQ=255)
                                                                                                                                                tCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGTGATGATc       >  1:5442062/1‑150 (MQ=255)
                                                                                                                                                tCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGTGATGATc       >  2:5356734/1‑150 (MQ=255)
                                                                                                                                                tCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGTGATGATc       >  1:2246328/1‑150 (MQ=255)
                                                                                                                                                   aGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGTGATGATCAAc    >  1:7717547/1‑150 (MQ=255)
                                                                                                                                                     ccTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGTGATGATCAACgg  >  1:8063938/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCGATGGCAGCGGCCACATACTCCAGCGCCTCGGGGGTTTCACCATGGGCCAGGGCGCGCTGCAACGCCACGTGCCACTGCTGCAGCGCGCCCATTGTGAGGGTAAGCGGGGTATCGGGAGAGGCCTGGGTCATGCCCGGCAGTCTAGCCTGCCAGGCACGCCCGCGCACAGCCCTTTGCGCCAGGTTCATCGGCCTTGGTACAGGCCAAGCGCCGTGAGTTCACGCGCGGTCTCAATGATGCACTGGCGCAGGGTCTCGACGATCTCGTCCACCTGCGCATGGGTGATGATCAACGG  >  NC_002947/4245091‑4245389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑