BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,345,077	C→T	35.4%	intergenic (+69/‑262)	ribAB‑II → / → PP_3814	3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,345,077	0	C	T	35.4%	169.0 / 142.4	144	intergenic (+69/‑262)	ribAB‑II/PP_3814	3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein
Reads supporting (aligned to +/- strand): ref base C (58/35); new base T (27/24); total (85/59)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01

Only 100 of 155 total aligned reads displayed.

GCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGC  >  NC_002947/4344930‑4345225

gcTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGAcacca                                                                                                                                                    <  2:889962/150‑1 (MQ=255)

     aaTACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGAc                                                                                                                                               <  2:4112326/150‑1 (MQ=255)

      aTACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACg                                                                                                                                              <  1:3926398/150‑1 (MQ=255)

             gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc                                                                                                                                       <  2:2345826/150‑1 (MQ=25)

             gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc                                                                                                                                       <  1:314474/150‑1 (MQ=25)

             gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc                                                                                                                                       <  1:78703/150‑1 (MQ=25)

             gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc                                                                                                                                       <  2:1563570/150‑1 (MQ=25)

             gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc                                                                                                                                       <  1:2008002/150‑1 (MQ=25)

             gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTcgc                                                                                                                                       <  2:666239/150‑1 (MQ=255)

              ggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgcg                                                                                                                                      <  2:4726886/150‑1 (MQ=25)

              ggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgcg                                                                                                                                      <  2:5988859/150‑1 (MQ=25)

              ggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTcgcg                                                                                                                                      <  1:8457497/150‑1 (MQ=255)

               gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACTCAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                     <  1:7406260/150‑1 (MQ=25)

               gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACCAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                     <  2:5986639/150‑1 (MQ=255)

               gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                     <  2:8098800/150‑1 (MQ=25)

               gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                     <  1:4787436/150‑1 (MQ=25)

               gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                     <  2:2182039/150‑1 (MQ=255)

               gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                     <  2:5012720/150‑1 (MQ=255)

               gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                     <  1:6945346/150‑1 (MQ=255)

               gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                     <  1:2930489/150‑1 (MQ=255)

               gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                     <  1:3434145/150‑1 (MQ=255)

               gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                     <  1:3589307/150‑1 (MQ=255)

                    cGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAACCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTaaa                                                                                                                                <  1:4179297/150‑1 (MQ=255)

                     gggCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAAc                                                                                                                               <  1:7092913/150‑1 (MQ=25)

                         tATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGc                                                                                                                           <  2:8147158/150‑1 (MQ=255)

                           tGAGCTGGTGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACACGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTc                                                                                                                         <  2:6484871/150‑1 (MQ=255)

                                    ggtggtGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGGTTTCCCGCGCATGCGAGGGGGGCACCACCGACGCCTTCGCGGGTAAACCCGCTCCTACAGGGc                                                                                                                >  2:8223122/1‑150 (MQ=38)

                                               gCAGCCGGTGCGACCGGGGTTTGGTGGGCTTACAACCCACACGCATTTTATTTCGGGGGGCCCGGTCGTCGGGGTTTTCCCCGCTATTGGGTGGGGGACACCCCGGACGCATCCGGGGTTAAACCCGCTCCTACAGGGCCCTTGGTGCtt                                                                                                     <  2:7746243/150‑1 (MQ=255)

                                                   cccGTTCGCCCCGGGGATGGTTGGATGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGTAGGGGGTTTACCCGCGAATGCGTGGGTGACACCACCGACGCATTCGCGGTTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGa                                                                                                 <  2:7242220/150‑1 (MQ=37)

                                                                  gatggttgGCTGAGAACCCACAAGACAGTGTTTTCGGGGGGCCCTGTCGGAGGGTGTTTCCCCGCGATGGCGTGGGTGACACCACCGACGATTTCGGGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATg                                                                                  <  2:8734793/150‑1 (MQ=25)

                                                                               gaACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACACCCCAAATTGAGGGTGAAACCAACGAAGCAATCGCCGGTAAAACCCCACCAACACGGCCCATGGTGCATTTTACATGCCGGCGGGATTGGCCGTCGCCCCa                                                                     >  2:6283547/1‑150 (MQ=255)

                                                                                   acacaAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTAACCGCGAATGCGAGCGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCGTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATAcc                                                                 >  2:7308888/1‑150 (MQ=255)

                                                                                   acacaAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTAACCCCCAATGCGAGCGTGACACCACCGAAGCATTAGCGGGTAAACCCGCTCCTACAGGGCCCGTGGTGCTTTTTACAGGGCGGAGTGATGGCCGGTCGCCCCAAAcc                                                                 >  2:7307881/1‑150 (MQ=38)

                                                                                   acacaAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTGACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATAcc                                                                 <  2:5817114/150‑1 (MQ=255)

                                                                                       aaGCATTTGATTTCGCGGGGCCCTGTCGGAGGGGGTTTACCCGCTTTTGGGCGGGTGACACCCCCGCCGCATGGGCGGGTACACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCtt                                                             <  2:6653168/150‑1 (MQ=255)

                                                                                       aaGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGAc                                                                                                                                               <  2:847908/68‑1 (MQ=255)

                                                                                       aaGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGAc                                                                                                                                               >  1:847908/1‑68 (MQ=255)

                                                                                                            ccTGTAGGAGCGGGTTTACCCGCGAATGCGGGGGTGACCCCACCGACGCCTTCGCGGGTAAACCCGCTCCTACAGGGCCCATGGTGCTTTTGGCAGGGCGGAGTGATGGCCGGGCGCCCCGTACCTCGTGCCCAAAAATTGGAATACCat                                        >  2:5801685/1‑150 (MQ=25)

                                                                                                             cTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATTGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCata                                       >  2:8754137/1‑150 (MQ=255)

                                                                                                                   gAGCGGGTTTACCCGCCAATGCGAGGGTGAAACCACCGAAGCATTAGCGGGTAAACCCGCTCCGACAGGGCCCTTGGTGCTTTTGTCAGGGCGGAGTGATGGCCCGTCGCCCCAAACCACATGCCAAAAACTTGGAATACCATAagatcg                                 >  2:6218834/1‑148 (MQ=255)

                                                                                                                     gCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTGGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGACTACCATAATATGatc                               >  2:8663176/1‑149 (MQ=255)

                                                                                                                        ggTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATcc                            >  2:233355/1‑150 (MQ=255)

                                                                                                                        ggTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATcc                            >  1:6779278/1‑150 (MQ=255)

                                                                                                                          tttACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCg                          >  1:287175/1‑150 (MQ=255)

                                                                                                                          tttACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCg                          >  1:970120/1‑150 (MQ=255)

                                                                                                                          tttACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCg                          >  1:4605803/1‑150 (MQ=255)

                                                                                                                            tACCCGCGACTGCGCGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTa                        <  2:4914562/150‑1 (MQ=255)

                                                                                                                             aCCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTc                                                                           >  1:6239885/1‑98 (MQ=255)

                                                                                                                               ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTCCAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  1:462805/1‑150 (MQ=25)

                                                                                                                               ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTc                                                                           <  2:1057093/96‑1 (MQ=14)

                                                                                                                               ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCACAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  2:7989975/1‑150 (MQ=25)

                                                                                                                               ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  1:5829792/1‑150 (MQ=25)

                                                                                                                               ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  1:2459116/1‑150 (MQ=25)

                                                                                                                               ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  2:639544/1‑150 (MQ=25)

                                                                                                                               ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  1:7866411/1‑150 (MQ=25)

                                                                                                                               ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  1:1437035/1‑150 (MQ=25)

                                                                                                                               ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  1:4827508/1‑150 (MQ=25)

                                                                                                                               ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  1:5653425/1‑150 (MQ=25)

                                                                                                                               ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCCTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATGCCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  2:256133/1‑150 (MQ=25)

                                                                                                                               ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  2:5937750/1‑150 (MQ=255)

                                                                                                                               ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  2:4813416/1‑150 (MQ=255)

                                                                                                                               ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  2:6418081/1‑150 (MQ=255)

                                                                                                                               ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  2:2468655/1‑150 (MQ=255)

                                                                                                                               ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  1:2532282/1‑150 (MQ=255)

                                                                                                                               ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  1:3448150/1‑150 (MQ=255)

                                                                                                                               ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  2:8353875/1‑150 (MQ=255)

                                                                                                                               ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  1:7365372/1‑150 (MQ=255)

                                                                                                                               ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  1:5342941/1‑150 (MQ=255)

                                                                                                                               ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGAGATGGCCGGTCGCCCCATACATCATGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                     >  1:7736873/1‑150 (MQ=255)

                                                                                                                                cgcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                    >  2:3174944/1‑150 (MQ=25)

                                                                                                                                cgcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                    >  2:309927/1‑150 (MQ=25)

                                                                                                                                cgcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                    >  1:1061085/1‑150 (MQ=25)

                                                                                                                                cgcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                    >  1:6556932/1‑150 (MQ=25)

                                                                                                                                cgcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCCTACCTCTTGCCAAAATCTTGGAATACCCTAATATGATATCCCGTAGAcc                    >  1:192061/1‑150 (MQ=255)

                                                                                                                                cgcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                    >  2:7773547/1‑150 (MQ=255)

                                                                                                                                cgcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                    >  1:4313937/1‑150 (MQ=255)

                                                                                                                                cgcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                    >  2:8183192/1‑150 (MQ=255)

                                                                                                                                 gcgAATGCGTCGGTGGTGTCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                   >  1:2508999/1‑150 (MQ=37)

                                                                                                                                 gcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                   >  1:1031387/1‑150 (MQ=25)

                                                                                                                                 gcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                   >  2:4942241/1‑150 (MQ=25)

                                                                                                                                 gcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                   >  2:2451542/1‑150 (MQ=25)

                                                                                                                                 gcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTTCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                   >  2:1336/1‑150 (MQ=255)

                                                                                                                                 gcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                   >  1:1758688/1‑150 (MQ=255)

                                                                                                                                 gcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                   >  2:1401698/1‑150 (MQ=255)

                                                                                                                                   gAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTg                 >  2:4822825/1‑150 (MQ=255)

                                                                                                                                   gAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTg                 >  2:426329/1‑150 (MQ=255)

                                                                                                                                   gAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTg                 >  1:1016344/1‑150 (MQ=255)

                                                                                                                                     aTGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAg               >  2:3914724/1‑150 (MQ=255)

                                                                                                                                       gCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATCTCCCGTAGACCTTGAGTc             >  2:7099031/1‑150 (MQ=255)

                                                                                                                                          ggaggtacacCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGc          <  2:6178248/144‑1 (MQ=255)

                                                                                                                                          ggaggtacacCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGc          <  2:8670158/144‑1 (MQ=255)

                                                                                                                                          cgggTGACACCCCCGACGCATGCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATCTGATATCCCGTAGACCTTGAGTCTGc          <  2:2511282/149‑1 (MQ=255)

                                                                                                                                               gACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGcc     >  2:6276031/1‑150 (MQ=255)

                                                                                                                                               gACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCGCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGcc     >  1:2753043/1‑150 (MQ=255)

                                                                                                                                                 caccacCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCgg   >  2:113310/1‑150 (MQ=255)

                                                                                                                                                  accaccGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGc  >  1:2775711/1‑150 (MQ=255)

                                                                                                                                                  accaccGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGc  >  1:7602334/1‑150 (MQ=255)

                                                                                                                                                  accaccGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGc  >  1:7602302/1‑150 (MQ=255)

                                                                                                                                                  accaccGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGc  >  1:5540694/1‑150 (MQ=255)

                                                                                                                                                  accaccGACGCATTCGCGGGTAAACCCGCTCCTAAAGGGCCCTTGGTGATTTAGACAGGGCGGAGTGATGTCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGc  >  1:1923810/1‑150 (MQ=255)

GCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGC  >  NC_002947/4344930‑4345225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`