BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,345,082	G→C	32.3%	intergenic (+74/‑257)	ribAB‑II → / → PP_3814	3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,345,082	0	G	C	32.3%	250.2 / 161.0	164	intergenic (+74/‑257)	ribAB‑II/PP_3814	3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein
Reads supporting (aligned to +/- strand): ref base G (71/40); new base C (29/24); total (100/64)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

Only 100 of 164 total aligned reads displayed.

AATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGCACCTA  >  NC_002947/4344935‑4345230

 aTACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACg                                                                                                                                                   <  1:3926523/150‑1 (MQ=255)

        gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc                                                                                                                                            <  1:314474/150‑1 (MQ=25)

        gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc                                                                                                                                            <  2:8147501/150‑1 (MQ=25)

        gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc                                                                                                                                            <  2:1106395/150‑1 (MQ=25)

        gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc                                                                                                                                            <  2:1374236/150‑1 (MQ=25)

        gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc                                                                                                                                            <  2:1563570/150‑1 (MQ=25)

        gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc                                                                                                                                            <  2:2345826/150‑1 (MQ=25)

        gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc                                                                                                                                            <  1:78703/150‑1 (MQ=25)

        gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCAGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc                                                                                                                                            <  2:5566004/150‑1 (MQ=25)

         ggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgcg                                                                                                                                           <  2:4726886/150‑1 (MQ=25)

         ggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTcgcg                                                                                                                                           <  1:8457497/150‑1 (MQ=255)

         ggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTcgcg                                                                                                                                           <  2:5981460/150‑1 (MQ=255)

          gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACTCAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  1:7406260/150‑1 (MQ=25)

          gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACCAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                          <  2:5986639/150‑1 (MQ=255)

          gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  1:6928750/150‑1 (MQ=25)

          gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  1:627445/150‑1 (MQ=25)

          gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  1:4787436/150‑1 (MQ=25)

          gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  1:2426181/150‑1 (MQ=25)

          gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  2:8098800/150‑1 (MQ=25)

          gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                          <  2:589582/150‑1 (MQ=255)

          gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                          <  2:730738/150‑1 (MQ=255)

          gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                          <  2:8761694/150‑1 (MQ=255)

          gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                          <  2:2182039/150‑1 (MQ=255)

          gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                          <  1:2930489/150‑1 (MQ=255)

          gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGGTCGACCCGGGGATGGATGGCTGAGAACACACACGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                          <  2:6490095/150‑1 (MQ=255)

               cGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTaaa                                                                                                                                     <  1:6054491/150‑1 (MQ=25)

               cGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAACCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTaaa                                                                                                                                     <  1:4179297/150‑1 (MQ=255)

                gggCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAAc                                                                                                                                    <  1:7092913/150‑1 (MQ=25)

                    tATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGc                                                                                                                                <  2:242284/150‑1 (MQ=21)

                    tATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGc                                                                                                                                <  2:8147158/150‑1 (MQ=255)

                      tGAGCTGGTGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACACGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTc                                                                                                                              <  2:6484871/150‑1 (MQ=255)

                               ggtggtGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGGTTTCCCGCGCATGCGAGGGGGGCACCACCGACGCCTTCGCGGGTAAACCCGCTCCTACAGGGc                                                                                                                     >  2:8223122/1‑150 (MQ=38)

                                              cccGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGCTTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGACGGGGACACCACCGACGCCGTCGCGGGGAAACCCGCGCCTACACGGCCCGTGGTGCAGTTGa                                                                                                      >  2:7973667/1‑150 (MQ=37)

                                                             gatggttgGCTGAGAACCCACAAGACAGTGTTTTCGGGGGGCCCTGTCGGAGGGTGTTTCCCCGCGATGGCGTGGGTGACACCACCGACGATTTCGGGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATg                                                                                       <  2:8734793/150‑1 (MQ=25)

                                                                          gaACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACACCCCAAATTGAGGGTGAAACCAACGAAGCAATCGCCGGTAAAACCCCACCAACACGGCCCATGGTGCATTTTACATGCCGGCGGGATTGGCCGTCGCCCCa                                                                          >  2:6283547/1‑150 (MQ=255)

                                                                              acacaAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTAACCGCGAATGCGAGCGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCGTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATAcc                                                                      >  2:7308888/1‑150 (MQ=255)

                                                                                acaAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGGTTAACCGCGAAAGCGAGGGGGACACCACCCCAGCAATCGCGGGTAAAACCCCACCCACCGGGCCCATGGTGCTTTTGACAGGGCGGAGTGGTTGCCCGTCGCCCCAAAccgc                                                                    >  2:4283363/1‑148 (MQ=255)

                                                                                  aaGCATTTGATTTCGCGGGGCCCTGTCGGAGGGGGTTTACCCGCTTTTGGGCGGGTGACACCCCCGCCGCATGGGCGGGTACACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCtt                                                                  <  2:6653168/150‑1 (MQ=255)

                                                                                  aaGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGAc                                                                                                                                                    <  2:847908/68‑1 (MQ=255)

                                                                                         tGATTTAGCGGGGCCCTGTAGGAGCGGGTTTAACCCCCAAAGCGAGGGTGACACCACCGACGCATTCGCGGGTAAAACCGCTCCTACAGGGCCCGTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCGCTTGCCaaaa                                                           >  2:8688907/1‑150 (MQ=38)

                                                                                                       ccTGTAGGAGCGGGTTTACCCGCGAATGCGGGGGTGACCCCACCGACGCCTTCGCGGGTAAACCCGCTCCTACAGGGCCCATGGTGCTTTTGGCAGGGCGGAGTGATGGCCGGGCGCCCCGTACCTCGTGCCCAAAAATTGGAATACCat                                             >  2:5801685/1‑150 (MQ=25)

                                                                                                              gAGCGGGTTTACCCGCGAAAGCGAGGGTGACACCACCGACGCATTCGCCGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCACTTGCCAAAATCTTGGAATACCATAATATGa                                      >  2:6391644/1‑150 (MQ=255)

                                                                                                              gAGCGGGTTTACCCGCCAATGCGAGGGTGAAACCACCGAAGCATTAGCGGGTAAACCCGCTCCGACAGGGCCCTTGGTGCTTTTGTCAGGGCGGAGTGATGGCCCGTCGCCCCAAACCACATGCCAAAAACTTGGAATACCATAagatcg                                      >  2:6218834/1‑148 (MQ=255)

                                                                                                                gCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGata                                    >  2:7408475/1‑150 (MQ=255)

                                                                                                                gCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGata                                    >  2:4814497/1‑150 (MQ=255)

                                                                                                                gCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTGGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGACTACCATAATATGatc                                    >  2:8663176/1‑149 (MQ=255)

                                                                                                                   ggTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATcc                                 >  1:6779278/1‑150 (MQ=255)

                                                                                                                     tttACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCg                               >  1:4605803/1‑150 (MQ=255)

                                                                                                                     tttACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCg                               >  1:287175/1‑150 (MQ=255)

                                                                                                                     tttACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCg                               >  1:970120/1‑150 (MQ=255)

                                                                                                                       tACCCGCGACTGCGCGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTa                             <  2:4914562/150‑1 (MQ=255)

                                                                                                                        aCCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTc                                                                                <  2:6239885/98‑1 (MQ=255)

                                                                                                                          ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTCCAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:462805/1‑150 (MQ=25)

                                                                                                                          ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTATAc                          >  2:6814745/1‑150 (MQ=25)

                                                                                                                          ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:2459116/1‑150 (MQ=25)

                                                                                                                          ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  2:2299343/1‑150 (MQ=25)

                                                                                                                          ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:1437035/1‑150 (MQ=25)

                                                                                                                          ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:4827508/1‑150 (MQ=25)

                                                                                                                          ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:5653425/1‑150 (MQ=25)

                                                                                                                          ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:7866411/1‑150 (MQ=25)

                                                                                                                          ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:867296/1‑150 (MQ=25)

                                                                                                                          ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCCTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATGCCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  2:256133/1‑150 (MQ=25)

                                                                                                                          ccGCGAATGCGGCGGTGGTGTCACCCTCGAATTCGCGGGTAAACCAGCTCCTACAGGACCCTTGGTGCTTTTGACAGGGCGGAGAGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  2:6144137/1‑150 (MQ=255)

                                                                                                                          ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  2:6418081/1‑150 (MQ=255)

                                                                                                                          ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:5342941/1‑150 (MQ=255)

                                                                                                                          ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:7365372/1‑150 (MQ=255)

                                                                                                                          ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:3448150/1‑150 (MQ=255)

                                                                                                                          ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:3448103/1‑150 (MQ=255)

                                                                                                                          ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:2532282/1‑150 (MQ=255)

                                                                                                                          ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  2:8353875/1‑150 (MQ=255)

                                                                                                                          ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  2:2468655/1‑150 (MQ=255)

                                                                                                                          ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGAGATGGCCGGTCGCCCCATACATCATGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:7736873/1‑150 (MQ=255)

                                                                                                                           cgcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                         >  1:3910382/1‑150 (MQ=25)

                                                                                                                           cgcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                         >  1:1061085/1‑150 (MQ=25)

                                                                                                                           cgcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                         >  2:309927/1‑150 (MQ=25)

                                                                                                                           cgcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                         >  1:192096/1‑150 (MQ=255)

                                                                                                                           cgcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                         >  1:4313937/1‑150 (MQ=255)

                                                                                                                           cgcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                         >  2:7773547/1‑150 (MQ=255)

                                                                                                                            gcgAATGCGTCGGTGGTGTCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                        >  1:2508999/1‑150 (MQ=37)

                                                                                                                            gcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                        >  1:1031387/1‑150 (MQ=25)

                                                                                                                            gcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                        >  2:4942241/1‑150 (MQ=25)

                                                                                                                            gcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTTCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                        >  2:1336/1‑150 (MQ=255)

                                                                                                                            gcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                        >  1:1758688/1‑150 (MQ=255)

                                                                                                                              gAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTg                      >  1:1016344/1‑150 (MQ=255)

                                                                                                                              gAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTg                      >  2:426329/1‑150 (MQ=255)

                                                                                                                                  gCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATCTCCCGTAGACCTTGAGTc                  >  2:7099031/1‑150 (MQ=255)

                                                                                                                                     cgggTGACACCCCCGACGCATGCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATCTGATATCCCGTAGACCTTGAGTCTGc               <  2:2511282/149‑1 (MQ=255)

                                                                                                                                          gACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCGCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGcc          >  1:2753043/1‑150 (MQ=255)

                                                                                                                                           acacCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCg         >  2:4009852/1‑150 (MQ=255)

                                                                                                                                            caccacCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCgg        >  2:3515760/1‑150 (MQ=255)

                                                                                                                                             accaccGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGc       >  1:2775711/1‑150 (MQ=255)

                                                                                                                                             accaccGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGc       >  1:7602334/1‑150 (MQ=255)

                                                                                                                                             accaccGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGc       >  1:7602302/1‑150 (MQ=255)

                                                                                                                                             accaccGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGc       >  1:5540694/1‑150 (MQ=255)

                                                                                                                                             accaccGACGCATTCGCGGGTAAACCCGCTCCTAAAGGGCCCTTGGTGATTTAGACAGGGCGGAGTGATGTCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGc       >  1:1923810/1‑150 (MQ=255)

                                                                                                                                               caccGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCt                                                        <  2:4823993/99‑1 (MQ=255)

                                                                                                                                               caccGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCt                                                        >  1:4823993/1‑99 (MQ=255)

                                                                                                                                                accGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGCAcc    >  2:4413061/1‑150 (MQ=255)

                                                                                                                                                accGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGCAcc    >  1:5983415/1‑150 (MQ=255)

                                                                                                                                                  cGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGCACCTa  >  2:3665044/1‑150 (MQ=255)

AATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGCACCTA  >  NC_002947/4344935‑4345230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`