BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,612,408	A→T	6.7%	K29M (AAG→ATG) ‡	PP_4079 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,612,408	0	A	T	6.7%	1223.7 / 51.8	375	K29M (AAG→ATG) ‡	PP_4079	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (195/154); new base T (7/18); total (202/173)
Fisher's exact test for biased strand distribution p-value = 1.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 380 total aligned reads displayed.

GGACCAGCTTCGTCGATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTGGAACCCAGCC  >  NC_002947/4612259‑4612557

     aGCTTCGTCGATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTgg                                                                                                                                                  <  2:3935104/150‑1 (MQ=255)

        ttCGTCGATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGgcgc                                                                                                                                               >  2:8369474/1‑150 (MQ=255)

          cgtcgATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGCGCCACCTTGGTGGCTCTg                                                                                                                                             <  2:6125291/150‑1 (MQ=255)

          cgtcgATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGCGCCACCTTGGTGGCTCTg                                                                                                                                             <  2:3520700/150‑1 (MQ=255)

          cgtcgATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCTTGGTGGCTCTg                                                                                                                                             <  1:1973719/150‑1 (MQ=255)

          cgtcgATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTg                                                                                                                                             <  1:1220470/150‑1 (MQ=255)

          cgtcgATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTg                                                                                                                                             <  2:6800657/150‑1 (MQ=255)

           gtcgATGACCACGATGGCCAACTCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCATGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGCGCCACCTTGGTGGCTCTgc                                                                                                                                            <  2:1455734/150‑2 (MQ=255)

            tcgATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGa                                                                                                                                           >  2:3377793/1‑150 (MQ=255)

              gATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGCGCCACCTTGGTGGCTCTgctca                                                                                                                                         <  1:1505294/150‑5 (MQ=255)

               ctGACCACGATGGCCAAATCAGTCCCATTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGCGCCACCTTGGTGGCTCTgctcaa                                                                                                                                        <  2:8012993/149‑6 (MQ=255)

                tGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCTTGGTGGCTCTgctcaag                                                                                                                                       <  1:4331256/150‑7 (MQ=255)

                 gACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACt                                                                                                                                      <  1:6937755/150‑1 (MQ=255)

                  aCCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTc                                                                                                                                     <  1:4700348/150‑1 (MQ=255)

                   ccACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCg                                                                                                                                    >  2:4058217/1‑150 (MQ=255)

                    cACGATGGCCAACTCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGt                                                                                                                                   <  1:4902436/150‑1 (MQ=255)

                    cACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGt                                                                                                                                   >  2:4175881/1‑150 (MQ=255)

                    cACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGt                                                                                                                                   >  2:2859181/1‑150 (MQ=255)

                    cACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGt                                                                                                                                   >  1:3709429/1‑150 (MQ=255)

                     aCGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGtt                                                                                                                                  <  1:816992/150‑1 (MQ=255)

                       gATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTAc                                                                                                                                <  1:1922110/150‑1 (MQ=255)

                       gATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTAc                                                                                                                                <  1:2504947/150‑1 (MQ=255)

                            ccAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATc                                                                                                                           >  2:6781326/1‑150 (MQ=255)

                             cAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCg                                                                                                                          >  2:5992996/1‑150 (MQ=255)

                              aaaTCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCgg                                                                                                                         <  2:226425/150‑1 (MQ=255)

                              aaaTCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCgg                                                                                                                         >  1:2109066/1‑150 (MQ=255)

                                       ccGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCaa                                                                                                                <  2:5697309/150‑1 (MQ=255)

                                        cGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAg                                                                                                               <  2:8729891/150‑1 (MQ=255)

                                        cGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAg                                                                                                               >  2:196405/1‑150 (MQ=255)

                                        cGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAg                                                                                                               >  2:1264117/1‑150 (MQ=255)

                                        cGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAg                                                                                                               >  1:4158730/1‑150 (MQ=255)

                                             tCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGAcaa                                                                                                          <  2:2378090/150‑1 (MQ=255)

                                             tCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGAcaa                                                                                                          <  2:7118158/150‑1 (MQ=255)

                                             tCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGAcaa                                                                                                          <  1:3543818/150‑1 (MQ=255)

                                             tCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGAcaa                                                                                                          >  2:656087/1‑150 (MQ=255)

                                             tCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGAcaa                                                                                                          >  1:7059261/1‑150 (MQ=255)

                                             tCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGACCGCCCAAGGAcaa                                                                                                          >  1:8677225/1‑150 (MQ=255)

                                               cGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGAcaacc                                                                                                        >  1:4055087/1‑150 (MQ=255)

                                                  ttCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGAcaacccaa                                                                                                     <  2:4339493/150‑1 (MQ=255)

                                                   tCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGAcaacccaac                                                                                                    <  1:7533880/150‑1 (MQ=255)

                                                        gggCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAg                                                                                               >  2:8501613/1‑150 (MQ=255)

                                                           ggctCATGA‑CCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTcgc                                                                                           >  2:7900873/4‑150 (MQ=255)

                                                            ggTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTcgc                                                                                           <  1:1364441/150‑1 (MQ=255)

                                                                aTGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTc                                                                                       >  2:6204775/1‑150 (MQ=255)

                                                                 tGAGCCGCCTGGCCGTCTGGGTTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCa                                                                                      <  2:8142668/150‑1 (MQ=255)

                                                                 tGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCa                                                                                      <  1:3842122/150‑1 (MQ=255)

                                                                  gAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAg                                                                                     <  2:1482578/150‑1 (MQ=255)

                                                                   aGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGc                                                                                    <  2:3863682/150‑1 (MQ=255)

                                                                   aGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGc                                                                                    >  2:4847930/1‑150 (MQ=255)

                                                                    gccgccTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCa                                                                                   <  1:8008768/150‑1 (MQ=255)

                                                                       gccTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCa                                                                                >  1:5502405/1‑150 (MQ=255)

                                                                        ccTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCaa                                                                               <  1:1465155/150‑1 (MQ=255)

                                                                        ccTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCaa                                                                               <  1:5196409/150‑1 (MQ=255)

                                                                        ccTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCaa                                                                               <  2:7369397/150‑1 (MQ=255)

                                                                                 cTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGccc                                                                      >  2:2982365/1‑150 (MQ=255)

                                                                                  tGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCa                                                                     >  1:784476/1‑150 (MQ=255)

                                                                                    gATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACTTCAGCCCAAc                                                                   <  1:2719450/150‑1 (MQ=255)

                                                                                       tGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCACCCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCt                                                                >  2:3748680/1‑150 (MQ=255)

                                                                                           ccTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAAc                                                            <  1:2788420/150‑1 (MQ=255)

                                                                                            ctctGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACa                                                           >  2:7839313/1‑150 (MQ=255)

                                                                                                 ctggtcgtgctggtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAAc                                                      >  1:1732013/1‑150 (MQ=255)

                                                                                                    gtcgtgctggtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCg                                                   >  1:6852725/1‑150 (MQ=255)

                                                                                                     tcgtgctggtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATTAACGTCAGCCCAACGCTCAACAGCAACCCGa                                                  <  2:8648747/150‑1 (MQ=255)

                                                                                                         gctggtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAg                                              >  2:5550439/1‑150 (MQ=255)

                                                                                                           tggtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAgtg                                            >  2:5681349/1‑150 (MQ=255)

                                                                                                            ggtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAgtgt                                           >  1:8719927/1‑150 (MQ=255)

                                                                                                            ggtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAgtgt                                           <  1:7439966/150‑1 (MQ=255)

                                                                                                            ggtcgAACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAgtgt                                           >  2:7654428/1‑150 (MQ=255)

                                                                                                             gtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCa                                                                                      <  2:4636776/106‑1 (MQ=255)

                                                                                                             gtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCa                                                                                      >  1:4636776/1‑106 (MQ=255)

                                                                                                             gtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTc                                          <  2:5408421/150‑1 (MQ=255)

                                                                                                             gtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTc                                          <  1:3377793/150‑1 (MQ=255)

                                                                                                               cgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGa                                        >  2:1772741/1‑150 (MQ=255)

                                                                                                               cgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGa                                        <  1:2338057/150‑1 (MQ=255)

                                                                                                                    tGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTgg                                   >  1:5905006/1‑150 (MQ=255)

                                                                                                                     gCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTggc                                  <  1:6259920/150‑1 (MQ=255)

                                                                                                                        aGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTggcggc                               >  1:1097705/1‑150 (MQ=255)

                                                                                                                          gTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCa                             >  1:8620477/1‑150 (MQ=255)

                                                                                                                          gTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCa                             >  2:6688244/1‑150 (MQ=255)

                                                                                                                            tGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATc                           <  2:5039965/150‑1 (MQ=255)

                                                                                                                               aCGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCt                        >  1:4526648/1‑150 (MQ=255)

                                                                                                                                 gTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTga                      >  2:1345474/1‑150 (MQ=255)

                                                                                                                                 gTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTga                      <  2:508081/150‑1 (MQ=255)

                                                                                                                                 gTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTga                      <  2:3185904/150‑1 (MQ=255)

                                                                                                                                 gTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTga                      <  1:3150245/150‑1 (MQ=255)

                                                                                                                                 gTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTga                      <  2:2164291/150‑1 (MQ=255)

                                                                                                                                 gTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTga                      >  1:8334845/1‑150 (MQ=255)

                                                                                                                                   ccTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCTACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTgaga                    <  1:3433673/150‑1 (MQ=255)

                                                                                                                                   ccTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTgaga                    <  1:8538973/150‑1 (MQ=255)

                                                                                                                                    cTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGcc                                                                       <  1:6675382/98‑1 (MQ=255)

                                                                                                                                    cTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGagag                   >  1:5640151/1‑150 (MQ=255)

                                                                                                                                     ttGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGc                  >  1:2380373/1‑150 (MQ=255)

                                                                                                                                     ttGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGc                  >  2:687055/1‑150 (MQ=255)

                                                                                                                                        tccAGCGCCACCTTGGTGGCTCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATc               >  1:7169528/3‑150 (MQ=255)

                                                                                                                                         gCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATcc              >  1:4396215/1‑150 (MQ=255)

                                                                                                                                           agagCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTg            >  2:5469221/1‑150 (MQ=255)

                                                                                                                                                caccaAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTGGAAcc       >  2:2183584/1‑150 (MQ=255)

                                                                                                                                                 accaAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTGGAAccc      >  2:934686/1‑150 (MQ=255)

                                                                                                                                                  ccaAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTGGAACCCa     <  1:5272050/150‑1 (MQ=255)

                                                                                                                                                  ccaAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTGGAACCCa     >  2:5804026/1‑150 (MQ=255)

GGACCAGCTTCGTCGATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTGGAACCCAGCC  >  NC_002947/4612259‑4612557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`