Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,865,803 G→T 5.6% L81M (CTG→ATG)  ligA ← DNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,865,8030GT 5.6% 1388.4 / 48.8 410L81M (CTG→ATG) ligADNA ligase
Reads supporting (aligned to +/- strand):  ref base G (204/183);  new base T (15/8);  total (219/191)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 412 total aligned reads displayed.

ACCAGCTGGCCGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCGGC  >  NC_002947/4865655‑4865950
                                                                                                                                                    |                                                                                                                                                   
    gCTGGCCGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGa                                                                                                                                                <  2:3928405/150‑1 (MQ=255)
    gCTGGCCGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGa                                                                                                                                                <  1:6214752/150‑1 (MQ=255)
        gCCGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCAt                                                                                                                                            >  2:3252385/1‑150 (MQ=255)
           gTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCATGCTCAGCCTggg                                                                                                                                         <  2:6100640/150‑1 (MQ=255)
              gcgGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCATGCTCAGCCTGGGCaa                                                                                                                                      <  2:87916/150‑2 (MQ=255)
              gcgGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCAc                                                                                                                                      <  1:578284/150‑1 (MQ=255)
                   tccgcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCCCGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCCGGCTGAGCATGGGCACTTCGt                                                                                                                                 <  2:5419900/147‑1 (MQ=255)
                    cagcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTg                                                                                                                                <  2:5287923/150‑1 (MQ=255)
                    cagcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTg                                                                                                                                <  2:2108222/150‑1 (MQ=255)
                      gcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGc                                                                                                                              <  2:6995557/150‑1 (MQ=255)
                      gcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGc                                                                                                                              >  1:2238419/1‑150 (MQ=255)
                            tCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCt                                                                                                                        <  1:7123809/150‑1 (MQ=255)
                            tCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCt                                                                                                                        >  1:3985858/1‑150 (MQ=255)
                                 gccaggccaTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTg                                                                                                                   >  2:6638026/1‑150 (MQ=255)
                                   caggccaTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGaa                                                                                                                 <  1:4430792/150‑1 (MQ=255)
                                   caggccaTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGaa                                                                                                                 >  2:4883397/1‑150 (MQ=255)
                                    aggccaTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAc                                                                                                                <  1:5555156/150‑1 (MQ=255)
                                      gccaTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcgc                                                                                                              <  2:6860109/150‑1 (MQ=255)
                                        caTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcgccg                                                                                                            >  2:1727042/1‑150 (MQ=255)
                                        caTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcgccg                                                                                                            >  1:5941599/1‑150 (MQ=255)
                                         aTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcgccgc                                                                                                           <  1:7008861/150‑1 (MQ=255)
                                          tCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACgccgcc                                                                                                          >  2:4447213/1‑150 (MQ=255)
                                            gAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCaa                                                                                                        <  1:7187058/150‑1 (MQ=255)
                                             aGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAg                                                                                                       <  2:4048000/150‑1 (MQ=255)
                                             aGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAg                                                                                                       >  2:3689341/1‑150 (MQ=255)
                                              gCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAgg                                                                                                      <  2:7062806/150‑1 (MQ=255)
                                               cTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGc                                                                                                     <  1:8437179/150‑1 (MQ=255)
                                                ttGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCa                                                                                                    >  2:7584670/1‑150 (MQ=255)
                                                 tGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAg                                                                                                   <  1:1891583/150‑1 (MQ=255)
                                                  gggTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGc                                                                                                  >  2:6419663/1‑150 (MQ=255)
                                                    gTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGc                                                                                                                              >  2:7284312/1‑120 (MQ=255)
                                                        gCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACGCACGCAGGTCGGCTGCGGCGCAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTccacc                                                                                            >  2:7410891/1‑150 (MQ=255)
                                                           gCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTccacccac                                                                                         <  1:5043836/150‑1 (MQ=255)
                                                             tGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCacac                                                                                       >  1:155685/1‑150 (MQ=255)
                                                                  tCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtgg                                                                                  >  2:2300130/1‑150 (MQ=255)
                                                                    gACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtgggt                                                                                <  1:5280144/150‑1 (MQ=255)
                                                                       cgcgCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtgggtggg                                                                             <  1:641975/150‑1 (MQ=255)
                                                                        gcgcCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGc                                                                            <  1:4251686/150‑1 (MQ=255)
                                                                          gcCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGa                                                                          >  2:6017064/1‑150 (MQ=255)
                                                                           cccGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAg                                                                         >  1:930118/1‑150 (MQ=255)
                                                                            ccGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGt                                                                        >  1:3096352/1‑150 (MQ=255)
                                                                             cGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTc                                                                       >  2:4644637/1‑150 (MQ=255)
                                                                              ggCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCg                                                                      <  2:345808/150‑1 (MQ=255)
                                                                                cTGATCGAGGCCTTCGACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCggg                                                                    <  2:773242/150‑1 (MQ=255)
                                                                                 tGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCgggg                                                                   >  1:4730530/1‑150 (MQ=255)
                                                                                  gATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCggggg                                                                  <  2:5866719/150‑1 (MQ=255)
                                                                                  gATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCATTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCggggg                                                                  >  2:8385230/1‑150 (MQ=255)
                                                                                   aTCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGt                                                                 >  2:3227163/1‑150 (MQ=255)
                                                                                   aTCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCATTGCCCAGGCTGAGCATGGGCACGTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGt                                                                 >  2:8136156/1‑150 (MQ=255)
                                                                                    tCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGtt                                                                >  1:6258794/1‑150 (MQ=255)
                                                                                    tCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGtt                                                                >  2:8039822/1‑150 (MQ=255)
                                                                                     cGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTa                                                               >  1:3392960/1‑150 (MQ=255)
                                                                                      gAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTAc                                                              >  2:1350817/1‑150 (MQ=255)
                                                                                           cTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGt                                                         >  2:2046923/1‑150 (MQ=255)
                                                                                            ttCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTg                                                        >  1:7797600/1‑150 (MQ=255)
                                                                                            ttCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTg                                                        >  1:3530069/1‑150 (MQ=255)
                                                                                            ttCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTg                                                        >  1:2047077/1‑150 (MQ=255)
                                                                                              cTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCg                                                      >  2:2617714/1‑150 (MQ=255)
                                                                                               tACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCgg                                                     <  2:5141543/150‑1 (MQ=255)
                                                                                                  cACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGgtg                                                  <  2:7952178/150‑1 (MQ=255)
                                                                                                     gcgACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTc                                               <  2:6782942/150‑1 (MQ=255)
                                                                                                     gcgACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTc                                               <  2:7539947/150‑1 (MQ=255)
                                                                                                     gcgACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTc                                               <  1:2417544/150‑1 (MQ=255)
                                                                                                        ccGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGc                                            <  2:4699873/149‑1 (MQ=255)
                                                                                                         cGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCt                                           <  2:4193683/150‑1 (MQ=255)
                                                                                                         cGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCt                                           <  2:7352416/150‑1 (MQ=255)
                                                                                                           gCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTc                                         >  2:8639122/1‑150 (MQ=255)
                                                                                                             cGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCa                                       >  1:4540222/1‑150 (MQ=255)
                                                                                                             cGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCa                                       >  1:29579/1‑150 (MQ=255)
                                                                                                              gAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAg                                      >  1:4841808/1‑150 (MQ=255)
                                                                                                              gAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAg                                      >  2:499013/1‑150 (MQ=255)
                                                                                                                  tCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCa                                       <  1:1180389/145‑1 (MQ=255)
                                                                                                                  tCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCa                                       >  2:1180389/1‑145 (MQ=255)
                                                                                                                    aCGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCtt                                >  2:939176/1‑150 (MQ=255)
                                                                                                                    aCGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCtt                                >  2:2673521/1‑150 (MQ=255)
                                                                                                                      gCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGa                              <  2:8281740/150‑1 (MQ=255)
                                                                                                                      gCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGa                              >  2:283473/1‑150 (MQ=255)
                                                                                                                         ggTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCt                           >  2:4251786/1‑150 (MQ=255)
                                                                                                                         ggTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCt                           <  1:6072831/150‑1 (MQ=255)
                                                                                                                             ggCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCTCACGCTGGGTGGGCGAGTCGGGGGTTTCCAGGTGCGGGTGTTCTGCTTCCAGCGCCTTGAGCTCGtt                       <  1:2767716/150‑1 (MQ=255)
                                                                                                                             ggCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGtt                       >  1:8605897/1‑150 (MQ=255)
                                                                                                                             ggCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGtt                       <  2:5677093/150‑1 (MQ=255)
                                                                                                                                ttcttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGaa                    >  1:103686/1‑150 (MQ=255)
                                                                                                                                ttcttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGaa                    >  1:3635454/1‑150 (MQ=255)
                                                                                                                                   ttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAg                 >  2:5811746/1‑150 (MQ=255)
                                                                                                                                   ttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAg                 >  1:6836450/1‑150 (MQ=255)
                                                                                                                                    tcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAgg                <  1:6513364/150‑1 (MQ=255)
                                                                                                                                     cGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGc               >  1:4126685/1‑150 (MQ=255)
                                                                                                                                      gAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCg              >  2:1125772/1‑150 (MQ=255)
                                                                                                                                       aaGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCgg             >  1:7469000/1‑150 (MQ=255)
                                                                                                                                         ggCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTc           <  2:3806290/150‑1 (MQ=255)
                                                                                                                                           cGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGt         >  2:2925064/1‑150 (MQ=255)
                                                                                                                                           aagtGCCCATGCTCAGCCTGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGt         >  2:7163105/4‑150 (MQ=255)
                                                                                                                                             ttGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTAt       <  1:7952203/150‑1 (MQ=255)
                                                                                                                                               gCCCAGGCTGCGCATGGGCACTGCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTc     <  2:5655584/150‑1 (MQ=255)
                                                                                                                                                ccccGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCg    >  1:1727319/1‑150 (MQ=255)
                                                                                                                                                cccAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCg    <  1:3907358/150‑1 (MQ=255)
                                                                                                                                                cccAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCg    >  1:6580639/1‑150 (MQ=255)
                                                                                                                                                 ccATGCTCAGCCTGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCgg   >  1:7288133/1‑150 (MQ=255)
                                                                                                                                                 ccAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCgg   <  1:3252385/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACCAGCTGGCCGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCGGC  >  NC_002947/4865655‑4865950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑