Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,975,605 G→C 5.0% intergenic (‑369/+25) fliC ← / ← PP_4379 flagellin/3‑oxoacyl‑ACP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,975,6050GC 5.0% 1011.6 / 30.7 297intergenic (‑369/+25)fliC/PP_4379flagellin/3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base G (140/142);  new base C (11/4);  total (151/146)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 314 total aligned reads displayed.

CGTCGGTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTGGAGGACACC  >  NC_002947/4975455‑4975753
                                                                                                                                                      |                                                                                                                                                    
 gTCGGTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGccgccg                                                                                                                                                      >  1:1005786/1‑150 (MQ=255)
    ggTGGAGCGGGTGAGGCTGCGATGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTCGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGgc                                                                                                                                                   <  1:3591926/150‑1 (MQ=255)
    ggTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGgc                                                                                                                                                   >  1:5220228/1‑150 (MQ=255)
    ggTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCAGTGTTCGCGGGTGAACCCGCTCCTACATTGGGCTGCGTTAGGCAGAAGATTATTTTCGGCAGAAAAAAAAGCGCCCGGCGGCCACCGCCCGgc                                                                                                                                                   <  2:3798429/150‑1 (MQ=38)
     gTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGgcg                                                                                                                                                  >  2:1176863/1‑150 (MQ=255)
          gCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCtttt                                                                                                                                             <  2:5411345/150‑1 (MQ=255)
           cGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTa                                                                                                                                            <  2:2859490/150‑1 (MQ=255)
              gTGAGGCTGTGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAggg                                                                                                                                         >  2:150848/1‑150 (MQ=255)
              gTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAggg                                                                                                                                         <  2:1962688/150‑1 (MQ=255)
                     tGCGAAGATGGAGACGCGTTGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACt                                                                                                                                  <  2:6608221/150‑1 (MQ=255)
                     tGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACt                                                                                                                                  >  1:8110746/1‑150 (MQ=255)
                     tGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACt                                                                                                                                  >  1:8612978/1‑150 (MQ=255)
                        gaagaaGGAGCCGCGATGGGTGGCACCGGCTGTGCCGGTGTTCGCGGGTGACCCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTggg                                                                                                                               <  2:5641318/150‑1 (MQ=255)
                         ccgaagGAGACGCGATGGGGGGCCCCGGCTGGGCCGGGGTTCGCGGGTGAACCCGCTCCTCCCAGGGGCTGCGTTAGGCAGACGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGa                                                                                                                              <  2:4514949/148‑1 (MQ=255)
                         aagaagGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGa                                                                                                                              <  2:7462887/150‑1 (MQ=255)
                            aagGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCa                                                                                                                           >  2:8549833/1‑150 (MQ=255)
                               gagaCGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCGTTTAGGGGCTGACTGGGATCGGtg                                                                                                                        >  1:7987057/1‑149 (MQ=255)
                                agaCGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTcg                                                                                                                       >  2:427632/1‑150 (MQ=255)
                                  ccgcgATGGGGGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGACGCTTATTTTCGGCATAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTcgcg                                                                                                                     <  1:5914244/149‑1 (MQ=255)
                                           gTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGAt                                                                                                            <  1:2281796/150‑1 (MQ=255)
                                            tGGCACCGGCTTTGCCGGTGTTCGCGGGTGACCCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACGGGATg                                                                                                           <  1:1754726/150‑1 (MQ=255)
                                            gaacACCGGCAAAGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGCTCAGTCGCGGTCCAGGATg                                                                                                           >  2:6858265/4‑150 (MQ=39)
                                              gCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGc                                                                                                         >  1:317856/1‑150 (MQ=255)
                                                aCCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCg                                                                                                       >  1:6173116/1‑150 (MQ=255)
                                                aCCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCCATAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCg                                                                                                       >  1:1101842/1‑150 (MQ=255)
                                                 cgggCTTTGCCTGTGTTCGCTGTTGAACCCGCTCCTACACGGGGCTGCGTTCGTCAGAAGTTTATTTTCGGAATAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCTGTACAGGATGGCCGa                                                                                                      <  1:5157007/148‑1 (MQ=255)
                                                 ccGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGa                                                                                                      >  2:3712971/1‑150 (MQ=255)
                                                 ccGGCTGTGCCGGGGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGGGTTCGGCCGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGa                                                                                                      <  1:972614/150‑1 (MQ=255)
                                                  cGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAg                                                                                                     >  2:8220146/1‑150 (MQ=255)
                                                   ggCTTTGCCGTTGTTCGCGTGTGAACACGCTCCTTCAATGGTGTGCGTTAGGCAGATGCTTATTTTCGTAAGAAAAAAACGCGCCGGGCGGTGTCCGCCGGGCGCATTTAGGGGCTGACTGGGATCATTCGCGGTACAGGATGGCCGAGc                                                                                                    <  1:6597641/150‑1 (MQ=255)
                                                        tGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCAc                                                                                               <  1:848599/150‑1 (MQ=255)
                                                          ccGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGa                                                                                             >  1:6110351/1‑150 (MQ=255)
                                                            ggTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACa                                                                                           <  2:7622469/150‑1 (MQ=255)
                                                             gtgtTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAg                                                                                          >  1:891394/1‑150 (MQ=255)
                                                                  cgcgGGTGAACCCGCTCCTACACTGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCa                                                                                     <  1:1596284/150‑1 (MQ=255)
                                                                      ggTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGcc                                                                                 <  1:7732392/150‑1 (MQ=255)
                                                                         taaCCCGCTCCTACACTGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCaaa                                                                              <  2:8128388/149‑1 (MQ=255)
                                                                         gAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCaaa                                                                              >  2:5361209/1‑150 (MQ=255)
                                                                         gAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCaaa                                                                              <  1:5714114/150‑1 (MQ=255)
                                                                         gAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCaaa                                                                              <  2:4821387/150‑1 (MQ=255)
                                                                          aaCCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAg                                                                             >  2:2453851/1‑150 (MQ=255)
                                                                             ccGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCa                                                                          >  2:1607030/1‑150 (MQ=255)
                                                                             ccGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCa                                                                          <  2:932824/150‑1 (MQ=255)
                                                                             ccGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCa                                                                          <  1:3847274/150‑1 (MQ=255)
                                                                             ccGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCa                                                                          >  1:2656602/1‑150 (MQ=255)
                                                                                   cTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATc                                                                    <  1:427632/150‑1 (MQ=255)
                                                                                   cTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGACAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATc                                                                    <  2:4419826/150‑1 (MQ=255)
                                                                                    tACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAATGCCACTGATCg                                                                   >  1:4161730/1‑150 (MQ=255)
                                                                                     aCAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGc                                                                  >  2:2198938/1‑150 (MQ=255)
                                                                                      cAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGcc                                                                 >  2:5049590/1‑150 (MQ=255)
                                                                                      cAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGcc                                                                 >  2:4903379/1‑150 (MQ=255)
                                                                                       caTGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCa                                                                <  2:7082237/149‑1 (MQ=255)
                                                                                       aaTGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCa                                                                <  2:6002381/150‑1 (MQ=255)
                                                                                       aaTGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCa                                                                >  2:4034397/1‑150 (MQ=255)
                                                                                        aTGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCAc                                                               <  2:3397295/150‑1 (MQ=255)
                                                                                              tGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCtt                                                         <  2:5802294/150‑1 (MQ=255)
                                                                                               gggTTTGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTc                                                        <  1:1439556/148‑1 (MQ=255)
                                                                                               gCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTc                                                        <  2:6286261/150‑1 (MQ=255)
                                                                                                cGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTcc                                                       <  2:4181137/150‑1 (MQ=255)
                                                                                                  ttAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAg                                                     >  1:4416163/1‑150 (MQ=255)
                                                                                                      gccgAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGc                                                 >  1:5456678/4‑150 (MQ=255)
                                                                                                      gCAGACGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGc                                                 <  2:839162/150‑1 (MQ=255)
                                                                                                      gCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGc                                                 >  1:7031977/1‑150 (MQ=255)
                                                                                                        aGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCa                                               >  1:4019397/1‑150 (MQ=255)
                                                                                                            gCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGa                                           <  1:319768/150‑1 (MQ=255)
                                                                                                            gCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGa                                           <  2:4647551/150‑1 (MQ=255)
                                                                                                              ttATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAACGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGc                                         >  2:55794/1‑150 (MQ=255)
                                                                                                              ttATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGc                                         >  2:2770716/1‑150 (MQ=255)
                                                                                                               tATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCa                                        <  2:8069548/150‑1 (MQ=255)
                                                                                                               tATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCGTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCa                                        >  1:5799570/1‑150 (MQ=255)
                                                                                                                aTTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCAt                                       <  1:5300275/150‑1 (MQ=255)
                                                                                                                       gCATAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTc                                <  1:7555536/150‑1 (MQ=255)
                                                                                                                       gCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCg                                            >  1:8787070/1‑138 (MQ=255)
                                                                                                                       gCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCg                                            <  2:8787016/138‑1 (MQ=255)
                                                                                                                       gCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTc                                <  2:6424254/150‑1 (MQ=255)
                                                                                                                         aGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTg                              >  1:6964563/1‑150 (MQ=255)
                                                                                                                         aGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTGTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTg                              >  1:2522304/1‑150 (MQ=255)
                                                                                                                          gAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTgc                             <  2:7786444/150‑1 (MQ=255)
                                                                                                                           aaaaaaaaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTgca                            <  2:5478470/150‑1 (MQ=255)
                                                                                                                           aaaaaaaaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTgca                            <  1:4531946/150‑1 (MQ=255)
                                                                                                                            aaaaaaaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTgcag                           >  1:8745112/1‑150 (MQ=255)
                                                                                                                             ctaaaaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTgcagc                          >  1:7388553/3‑150 (MQ=255)
                                                                                                                             ctaaaaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTgcagc                          >  2:510849/3‑150 (MQ=255)
                                                                                                                             aaaaaaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACACCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTgcagc                          >  1:343458/1‑150 (MQ=255)
                                                                                                                                 aaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTgcagcagca                      >  1:2787655/1‑150 (MQ=255)
                                                                                                                                 aaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTgcagcagca                      >  1:1328610/1‑150 (MQ=255)
                                                                                                                                 aaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTgcagcagca                      >  1:4931529/1‑150 (MQ=255)
                                                                                                                                  aGCGCCCGGCGGCCACCGCCCGGCGCTTTTTGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGcagcagcag                     >  2:308573/1‑150 (MQ=255)
                                                                                                                                   gcgcCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCagcagcagc                    <  1:2119495/150‑1 (MQ=255)
                                                                                                                                           cGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACt            >  1:4652366/1‑150 (MQ=255)
                                                                                                                                            ggTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTg           <  2:2015906/150‑1 (MQ=255)
                                                                                                                                            ggTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTg           <  2:1418379/150‑1 (MQ=255)
                                                                                                                                             ggggCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTgg          <  2:6806747/148‑1 (MQ=255)
                                                                                                                                             gTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTgg          <  2:7385358/150‑1 (MQ=255)
                                                                                                                                              tGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTgga         <  1:2943243/150‑1 (MQ=255)
                                                                                                                                              tGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTgga         <  2:6150169/150‑1 (MQ=255)
                                                                                                                                              ccaccgcccGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTgga         >  1:5707701/4‑150 (MQ=255)
                                                                                                                                               caccgcccGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTggag        <  2:3768975/148‑1 (MQ=255)
                                                                                                                                                  cgccgGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTGGAGGac     >  2:1208414/1‑150 (MQ=255)
                                                                                                                                                    ccgGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTGGAGGacac   <  1:5015732/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
CGTCGGTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTGGAGGACACC  >  NC_002947/4975455‑4975753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑