Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 385,478 A→C 10.4% intergenic (+15/+20) PP_0320 → / ← ltaE methyl‑accepting chemotaxis transducer/low specificity l‑threonine aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947385,4780AC10.4% 742.4 / 43.7 259intergenic (+15/+20)PP_0320/ltaEmethyl‑accepting chemotaxis transducer/low specificity l‑threonine aldolase
Reads supporting (aligned to +/- strand):  ref base A (131/97);  new base C (6/24);  total (137/122)
Fisher's exact test for biased strand distribution p-value = 1.40e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

AACCGGACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGCCCCGC  >  NC_002947/385329‑385627
                                                                                                                                                     |                                                                                                                                                     
aaCCGGACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCa                                                                                                                                                       >  1:5141750/1‑150 (MQ=255)
aaCCGGACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCa                                                                                                                                                       >  2:5548025/1‑150 (MQ=255)
aaCCGGACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCa                                                                                                                                                       <  1:6960240/150‑1 (MQ=255)
aaCCGGACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCa                                                                                                                                                       >  2:6699561/1‑150 (MQ=255)
    ggACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCAcggc                                                                                                                                                   >  1:7850972/1‑150 (MQ=255)
    ggACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCAcggc                                                                                                                                                   <  2:3434781/150‑1 (MQ=255)
    ggACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCAcggc                                                                                                                                                   <  1:2381206/150‑1 (MQ=255)
          tGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGcccc                                                                                                                                             >  2:85302/1‑150 (MQ=255)
               gggATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGctc                                                                                                                                        >  2:842594/1‑150 (MQ=255)
                ggATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGctct                                                                                                                                       <  2:609113/150‑1 (MQ=255)
                ggATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGctct                                                                                                                                       <  2:609067/150‑1 (MQ=255)
                    ttCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCt                                                                                                                                   >  1:8706897/1‑150 (MQ=255)
                      ccGATTTGGCGCGTGAGACGGCGGCGGAGGTGAAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCa                                                                                                                                 <  2:1231495/150‑1 (MQ=255)
                        gATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGc                                                                                                                               >  1:6904747/1‑150 (MQ=255)
                        gATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGc                                                                                                                               <  2:4254341/150‑1 (MQ=255)
                                  gTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGAt                                                                                                                     >  1:6380288/1‑150 (MQ=255)
                                   tGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATc                                                                                                                    <  2:7900718/150‑1 (MQ=255)
                                     agaCGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGt                                                                                                                  >  1:1930074/1‑150 (MQ=255)
                                     agaCGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGt                                                                                                                  >  2:583255/1‑150 (MQ=255)
                                      gaCGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTg                                                                                                                 >  2:5290630/1‑150 (MQ=255)
                                      gaCGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTg                                                                                                                 >  2:900632/1‑150 (MQ=255)
                                      gaCGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTg                                                                                                                 >  2:5545838/1‑150 (MQ=255)
                                       aCGGCGGCGGAGGTGCATGGGTGCCGCGAGGAGTGCCAGACGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGGTGGGTGGGTTCAGGCTCTAGATCGCTGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGc                                                                                                                <  2:2366805/150‑1 (MQ=255)
                                        cggcggcggAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCg                                                                                                               <  2:6849666/150‑1 (MQ=255)
                                              cggAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATc                                                                                                         >  1:3867267/1‑150 (MQ=255)
                                                  ggTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTCGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATccgcc                                                                                                     <  2:5964189/150‑1 (MQ=255)
                                                    tGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCcgccgc                                                                                                   >  1:4821550/1‑150 (MQ=255)
                                                    tGCAAGGGTGCCGCGAGGAGTGACATGCGTTGCGTGGGTTGGCTGATGACCTGTCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCcgccgc                                                                                                   >  1:5661249/1‑150 (MQ=255)
                                                      cAAGGGTGCCGCGTGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCa                                                                                                 <  1:8636186/150‑1 (MQ=255)
                                                       aaGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAg                                                                                                >  1:6995609/1‑150 (MQ=255)
                                                        cgggTGCCGCGCGGCGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCCGCGGGGTGGGTTCCGGCTCTAGATCGCCGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGc                                                                                               <  2:3861907/149‑1 (MQ=255)
                                                          ggTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTc                                                                                             >  1:6103491/1‑150 (MQ=255)
                                                              ccGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGc                                                                                         <  2:5141195/150‑1 (MQ=255)
                                                                gcgAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCAc                                                                                       >  2:70450/1‑150 (MQ=255)
                                                                gcgAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCAc                                                                                       >  1:188765/1‑150 (MQ=255)
                                                                gcgAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCAc                                                                                       >  2:3753910/1‑150 (MQ=255)
                                                                   aggagTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCcg                                                                                    >  2:8120721/1‑150 (MQ=255)
                                                                        tGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGctt                                                                               >  2:1783592/1‑150 (MQ=255)
                                                                        tGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGctt                                                                               >  2:1486882/1‑150 (MQ=255)
                                                                          ccAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGcttct                                                                             >  1:6745855/1‑150 (MQ=255)
                                                                            tgggtttGGGTGGGTGGGCTTATGCCGGGGCGGGGCTTTTGGGTGGTTTCTGGCTCTAGATCGCCGGGGCCGCCGGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCttcttc                                                                           <  1:4400411/145‑1 (MQ=255)
                                                                            tggggTTGGGTGGGTGGGCTTATGACGGGGCGGGGCTGTGGGGTGGTTTCCGGCTCTTGAGCGCTGGGGCCGCCGGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCttcttc                                                                           <  1:7418884/146‑1 (MQ=255)
                                                                            cggCGTTGCGTGGGTTGGCTGTTGCCGTGGCGCGGCTTTTGGGTGGGTTCAGGCTCTAGATCGCGGGGGCCGCCGGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTAACGCACCCGCGCttcttc                                                                           <  1:4134219/149‑1 (MQ=255)
                                                                            aGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCttcttc                                                                           <  2:3908737/150‑1 (MQ=255)
                                                                            aGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCttcttc                                                                           <  2:3213573/150‑1 (MQ=255)
                                                                             ggCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCg                                                                          <  2:7655252/150‑1 (MQ=255)
                                                                              gctttGGGTGGGTTGGCTGATGCCGTGGCGCGGCTGTTGGGTGGTTTCTGGCTCTAGATCGCCGGGGCCGCCCGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCgg                                                                         <  1:3982339/147‑1 (MQ=255)
                                                                                 ttGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTAt                                                                      >  2:8301296/1‑150 (MQ=255)
                                                                                   gCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCCGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATcc                                                                    <  2:3994155/150‑1 (MQ=255)
                                                                                     gTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCa                                                                  >  2:1095993/1‑150 (MQ=255)
                                                                                     gTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCa                                                                  >  2:2676397/1‑150 (MQ=255)
                                                                                      tGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCAc                                                                 <  2:6553793/150‑1 (MQ=255)
                                                                                      tGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCAc                                                                 >  1:2350703/1‑150 (MQ=255)
                                                                                        ggTTGGCTGATGACCTGGCGCGGCAGTTGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGa                                                               <  1:3361792/150‑1 (MQ=255)
                                                                                        ggTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGa                                                               <  2:2096558/150‑1 (MQ=255)
                                                                                              cTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACAt                                                         >  2:2032587/1‑150 (MQ=255)
                                                                                              cTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACAt                                                         >  2:3287734/1‑150 (MQ=255)
                                                                                               tgatgaCCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATg                                                        >  1:25554/1‑150 (MQ=255)
                                                                                                gatgaCCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACAGGa                                                       >  1:6224748/1‑150 (MQ=255)
                                                                                                 atgaCCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGaa                                                      <  2:2150260/150‑1 (MQ=255)
                                                                                                   gaCCTGGCGCGGCAGTTGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAgc                                                    <  2:3066369/150‑1 (MQ=255)
                                                                                                   gaCCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAgc                                                    >  1:1025068/1‑150 (MQ=255)
                                                                                                      cTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGAGCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGGATCCCACGAGCACATGAAgcgcg                                                 >  1:6503695/1‑150 (MQ=255)
                                                                                                       tGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAgcgcgc                                                >  1:1149266/1‑150 (MQ=255)
                                                                                                        ggCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAgcgcgcg                                               >  1:2318163/1‑150 (MQ=255)
                                                                                                         gcgcGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAgcgcgcgc                                              >  2:4023113/1‑150 (MQ=255)
                                                                                                           gcgGCAGATGGGTGGGTTCTGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCa                                            <  2:4821550/150‑1 (MQ=255)
                                                                                                              gCAGAGGGGTGGGTTCAGGCTCTAGATCGCCGGGGCCGCCCGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCg                                         <  1:5483220/150‑1 (MQ=255)
                                                                                                                ttaggggtgggtTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCt                                       <  1:992128/146‑1 (MQ=255)
                                                                                                                aGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCt                                       <  1:5871056/150‑1 (MQ=255)
                                                                                                                  aTGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGc                                     <  2:2903029/150‑1 (MQ=255)
                                                                                                                   tgggtgggTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACACACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGcc                                    >  1:7976095/1‑150 (MQ=255)
                                                                                                                      gtgggtTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGAt                                 >  1:3604866/1‑150 (MQ=255)
                                                                                                                      gtgggtTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGAt                                 >  2:3327368/1‑150 (MQ=255)
                                                                                                                      gtgggtTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGAt                                 >  1:5285312/1‑150 (MQ=255)
                                                                                                                         ggtTCCGGCTCTAGATCGCTGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATaaa                              <  1:1633701/150‑1 (MQ=255)
                                                                                                                         ggtTCAGGCTCTAGATCGCCGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATaaa                              <  1:7918795/150‑1 (MQ=255)
                                                                                                                         ggtTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATaaa                              <  2:6943517/150‑1 (MQ=255)
                                                                                                                          tgtcgagCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAg                             <  2:4090998/144‑1 (MQ=255)
                                                                                                                          gtTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAg                             <  2:8706897/150‑1 (MQ=255)
                                                                                                                           ttCAGGCTCTAGATCGCAGGGGCCGCACGGTGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCGTCGAGCACATGAAGCGCGCGCCACCTCTGCCGATAAAgg                            <  2:5642807/150‑1 (MQ=255)
                                                                                                                           ttCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAgg                            >  1:1738991/1‑150 (MQ=255)
                                                                                                                           ttCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAgg                            >  2:1153420/1‑150 (MQ=255)
                                                                                                                             ctggCTCTTGTTCGCCGGGGCCGCACGGGGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGta                          <  1:3220991/148‑1 (MQ=255)
                                                                                                                                gCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAg                       >  1:203170/1‑150 (MQ=255)
                                                                                                                                gCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAg                       >  1:7710088/1‑150 (MQ=255)
                                                                                                                                 ctctAGATCGCCGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGa                      <  2:7422580/150‑1 (MQ=255)
                                                                                                                                       agagccggggCCGCCGTGCGGCCCCAGCTCTCGCTCAGCCACCAAAGATCGTGCGGATATCCGCCGCCAGCTCACCCACCCGCGCGTCTTCGGTATCCCACGAGCACAGGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGcc                >  2:57279/7‑150 (MQ=255)
                                                                                                                                           ccggggCCGCCGTGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGcc            >  1:3040057/3‑150 (MQ=255)
                                                                                                                                           cAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGcc            >  1:663760/1‑150 (MQ=255)
                                                                                                                                            cggggCCGCCGTGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGccc           >  1:7515561/2‑150 (MQ=255)
                                                                                                                                            aGGGGACGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGccc           <  2:2459714/150‑1 (MQ=255)
                                                                                                                                             ggggCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCt          >  2:4389252/1‑150 (MQ=255)
                                                                                                                                              gggCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCtt         >  1:4633776/1‑150 (MQ=255)
                                                                                                                                                gCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGc       >  1:1110749/1‑150 (MQ=255)
                                                                                                                                                 ccGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGcc      <  2:4735302/150‑1 (MQ=255)
                                                                                                                                                  cGCAGGGGGCCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGccc     <  1:2708/150‑1 (MQ=255)
                                                                                                                                                  cGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGccc     >  2:7613318/1‑150 (MQ=255)
                                                                                                                                                   gCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTCTAGAAGCGCCAGCCCTTGcccc    >  2:6385732/1‑150 (MQ=255)
                                                                                                                                                     aCGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGCCCCGc  >  1:5778745/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACCGGACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGCCCCGC  >  NC_002947/385329‑385627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑