BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,084,426	G→T	7.5%	intergenic (‑51/+24)	PP_t55 ← / ← PP_t54	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,084,426	0	G	T	7.5%	488.5 / 15.9	166	intergenic (‑51/+24)	PP_t55/PP_t54	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base G (86/64); new base T (4/11); total (91/75)
Fisher's exact test for biased strand distribution p-value = 2.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

AAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAAGCAGCCTT  >  NC_002947/5084277‑5084575

aaGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTTATATGGCTTTg                                                                                                                                                       >  2:3359155/1‑150 (MQ=255)

 aGCAGACCGACTTCACGAAAGTGGCGGTCTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGc                                                                                                                                                      <  1:925356/150‑1 (MQ=255)

 aGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGc                                                                                                                                                      <  1:66888/150‑1 (MQ=255)

   cAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCaa                                                                                                                                                    <  2:3496872/150‑1 (MQ=255)

      aaCCACTTCAAGAAAGTGGCGGACTCAGCAGGAATCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCa                                                                                                                                                 <  1:2693192/150‑1 (MQ=255)

        ccACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGa                                                                                                                                               <  2:4505386/150‑1 (MQ=255)

        ccACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGa                                                                                                                                               <  1:6167031/150‑1 (MQ=255)

           cTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAAcc                                                                                                                                            <  1:6356935/150‑1 (MQ=255)

            ttCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTCAAAGCAGAACCa                                                                                                                                           >  2:1165222/1‑150 (MQ=255)

            ttCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAAAGTCTGTGCAAGCCGAACCa                                                                                                                                           >  2:7180654/1‑150 (MQ=255)

                aGATAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTc                                                                                                                                       <  1:7427586/150‑1 (MQ=255)

                 gAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCa                                                                                                                                      <  1:2810725/150‑1 (MQ=255)

                  aaaGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCaa                                                                                                                                     <  1:2182823/150‑1 (MQ=255)

                  aaaGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCCCTGGTTGACTGAAAAGGCTTTGCAAGCAGAACAACTTCaa                                                                                                                                     >  2:8780963/1‑150 (MQ=255)

                      tGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGaaa                                                                                                                                 <  1:7588693/150‑1 (MQ=255)

                      tGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGaaa                                                                                                                                 <  1:6285355/150‑1 (MQ=255)

                        gCGGACTCAGCAGGATTCGACCCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGt                                                                                                                               <  1:1204463/150‑1 (MQ=255)

                          ggACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTgg                                                                                                                             >  2:4450024/1‑150 (MQ=255)

                            aCTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCg                                                                                                                           <  1:1229513/150‑1 (MQ=255)

                            aCTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCg                                                                                                                           <  1:7871126/150‑1 (MQ=255)

                              tCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGa                                                                                                                         <  1:1512716/150‑1 (MQ=255)

                              tCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGa                                                                                                                         <  1:3413568/150‑1 (MQ=255)

                              tCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGa                                                                                                                         <  1:7480212/150‑1 (MQ=255)

                              tCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCGGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGa                                                                                                                         >  2:5253876/1‑150 (MQ=255)

                                agcagGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACt                                                                                                                       >  2:3108886/1‑150 (MQ=255)

                                agcagGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGAATGAACAGGCATTGCAAGCAGAACCACTTCAAGAAAGTGGAGTACt                                                                                                                       >  1:3227159/1‑150 (MQ=17)

                                 gcagGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATAACCGATGGTTGAATGAAAAGCCTTTGAAAGAAGAACCACTACAAGAAAGAGGCGGACTc                                                                                                                      >  2:7536306/1‑150 (MQ=17)

                                     gATTCGCACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTcagca                                                                                                                  <  1:813766/150‑1 (MQ=255)

                                       ttCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAgg                                                                                                                >  2:8574592/1‑150 (MQ=255)

                                          gAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGAtt                                                                                                             >  2:3773510/1‑150 (MQ=255)

                                          gAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGAtt                                                                                                             >  1:494786/1‑150 (MQ=255)

                                          gAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGAtt                                                                                                             >  2:8594946/1‑150 (MQ=255)

                                                  gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATAACCGCTGGTTGACGGACCAGGCTTTGCAAGCAGAACCACTGCAAGAGCGGGGCGGACGCAGCAGGAGTCGCAccgg                                                                                                     >  2:8289086/1‑148 (MQ=17)

                                                     cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGa                                                                                                  <  1:3323522/150‑1 (MQ=255)

                                                      gCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGAc                                                                                                 <  1:8566885/150‑1 (MQ=255)

                                                       cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTAAGCAGGATTCGAACCTGCGAcc                                                                                                >  2:7617165/1‑150 (MQ=255)

                                                        tCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCg                                                                                               <  1:6605978/150‑1 (MQ=255)

                                                        tCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCg                                                                                               >  1:8029211/1‑150 (MQ=255)

                                                                tAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTc                                                                                       >  2:2296689/1‑150 (MQ=255)

                                                                tAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGAGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTTAAATGGCTTTGCAAGCAGAAACACTTCAAGAAATTGGAGGACTCAGCAGGATTCGAACCTGAGACCGCTCGGTTc                                                                                       >  1:3724973/1‑150 (MQ=17)

                                                                   ccGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCATTATTAAACCAGATTACCGCTGGTTGACTGAACTGCCTTTGCAAGCAGAACCACTGCAAGAAAGTGGCGGACTCAGCAGTATTCGAACCAGCGACCGATCGGATCTTa                                                                                    >  1:3165722/1‑150 (MQ=17)

                                                                   ccGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTa                                                                                    <  2:1550421/150‑1 (MQ=255)

                                                                   ccGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTa                                                                                    >  2:3054303/1‑150 (MQ=255)

                                                                    cGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAg                                                                                   >  1:1527411/1‑150 (MQ=255)

                                                                    cGAGGACTCCATCCAGCCGAGCTATGAGTCCGCGATTGGCAGTTTTACACCAGCTTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAg                                                                                   <  2:3474103/150‑1 (MQ=17)

                                                                        tACTCTATCCATCTGAGCTATGAGTCCGCGATTGGCAGTTTTAACCCAGATGACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGACCCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGa                                                                               <  2:6234612/150‑1 (MQ=255)

                                                                        tACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGa                                                                               <  2:5495498/150‑1 (MQ=255)

                                                                        tACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGa                                                                               <  2:2135872/150‑1 (MQ=255)

                                                                          ctctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGt                                                                             >  2:2342479/1‑150 (MQ=255)

                                                                          ctctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGt                                                                             >  2:8214153/1‑150 (MQ=255)

                                                                           tctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTa                                                                            >  2:1938070/1‑150 (MQ=255)

                                                                           tctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTa                                                                            >  1:8395302/1‑150 (MQ=255)

                                                                           tctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTa                                                                            >  1:8207998/1‑150 (MQ=255)

                                                                           tctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTa                                                                            >  1:7688787/1‑150 (MQ=255)

                                                                             tATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGc                                                                                  <  1:3673158/142‑1 (MQ=255)

                                                                             tATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGc                                                                                  >  2:3673158/1‑142 (MQ=255)

                                                                             tATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTAct                                                                          >  1:4487134/1‑150 (MQ=255)

                                                                             tATCCAGCTGAGCTATGAGTCCGCGAGTGGAAGTATTAAAACACATTACAGCTGGTTTACAGAAATGTCTTGGCAAGCAGAAACACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTAct                                                                          >  1:1297692/1‑150 (MQ=14)

                                                                               tCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTActct                                                                        >  2:8306695/1‑150 (MQ=255)

                                                                                ccAGCAGCGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                                                       <  2:6525073/150‑1 (MQ=255)

                                                                                       aGCTATGACTCCGCTATTGGGAGTTTTAAACCAGCTTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                                <  2:8390120/150‑1 (MQ=255)

                                                                                        gCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                               >  1:4653512/1‑150 (MQ=255)

                                                                                         gtaattgTCCGCTTTTGGCAGTTTTATACCAGATTACCTCTGGTTTACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                              <  2:7401440/144‑1 (MQ=14)

                                                                                                tCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGa                                                       >  1:2447674/1‑150 (MQ=255)

                                                                                                tCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGa                                                       >  1:2922704/1‑150 (MQ=255)

                                                                                                tCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGa                                                       <  2:8207998/150‑1 (MQ=255)

                                                                                                   gcgATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGAGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAgag                                                    >  1:2939937/1‑148 (MQ=255)

                                                                                                     ggttGGTAGTTTTATACCAGTTTACCGCTGGTTGACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCg                                                  <  2:7738285/148‑1 (MQ=255)

                                                                                                         ggCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCgacag                                              >  2:7023459/1‑146 (MQ=255)

                                                                                                         ggCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAAt                                              >  1:6630945/1‑150 (MQ=255)

                                                                                                          gCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGaaaat                                             >  1:5631068/1‑148 (MQ=255)

                                                                                                          gCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGACCGCAAtt                                             >  1:6871955/1‑150 (MQ=255)

                                                                                                               tttAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAg                                        >  2:1501976/1‑150 (MQ=255)

                                                                                                                   aaCCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGtttt                                    >  1:5565533/1‑150 (MQ=255)

                                                                                                                    aCCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTa                                   <  2:3984125/150‑1 (MQ=255)

                                                                                                                      cAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGa                                 >  1:5410329/1‑150 (MQ=255)

                                                                                                                              cGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGTAGTTTTAGACCAGATAc                         >  2:8551422/1‑150 (MQ=255)

                                                                                                                                  ggTTGTCTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCt                     <  1:3576024/150‑1 (MQ=255)

                                                                                                                                   gTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTg                    >  2:7210464/1‑150 (MQ=255)

                                                                                                                                     tGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGt                  <  1:7015839/150‑1 (MQ=255)

                                                                                                                                       aCTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAAATGGCAGTTATAGACCAGATACCGCTGGtgg                >  2:7034307/1‑148 (MQ=255)

                                                                                                                                        cTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCCATTGGCAGAGTTAGAACAGAGACAGAAGGttga               >  2:8048020/1‑150 (MQ=11)

                                                                                                                                        cTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttga               <  2:5582084/150‑1 (MQ=40)

                                                                                                                                        cTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGAAACCGCTGGttga               >  1:4347468/1‑150 (MQ=255)

                                                                                                                                         tGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttgat              >  1:2155877/1‑150 (MQ=255)

                                                                                                                                         tGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttgat              >  1:1743410/1‑150 (MQ=255)

                                                                                                                                         tGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttgat              >  2:923732/1‑150 (MQ=255)

                                                                                                                                         tGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttgat              >  1:5323645/1‑150 (MQ=255)

                                                                                                                                         tGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAAATTGCAGGTTAAGAAAAGTAACCGATGGttgat              >  1:4382115/1‑150 (MQ=11)

                                                                                                                                          gAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttgatt             <  2:4531071/150‑1 (MQ=38)

                                                                                                                                          gAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttgatt             <  2:3451714/150‑1 (MQ=38)

                                                                                                                                           aaaTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGa                                 <  2:1855747/129‑1 (MQ=14)

                                                                                                                                             ttGGCTTTTCAACCAGAACCACTTAAACACCGTGGCTCACACCCCATGATCCGAACCCGCCACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGaa          <  1:1869587/149‑1 (MQ=11)

                                                                                                                                             aTGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGaa          <  1:3697796/150‑1 (MQ=255)

                                                                                                                                             aTGGCTTTGAAACCACAACCCCTTCACGACCGGGGCGCACTCAGCAGGATTCGAACCGGCCCCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGaa          <  1:1869589/150‑1 (MQ=14)

                                                                                                                                              ttgCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAag         <  1:7180654/148‑1 (MQ=255)

                                                                                                                                              tGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAag         >  1:8756270/1‑150 (MQ=255)

                                                                                                                                                gCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAagca       >  2:7995688/1‑150 (MQ=255)

                                                                                                                                                gCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAAGTGGCAGGTGTAGACCAGATACCGCTGGTGGATTGAagca       >  2:277120/1‑150 (MQ=255)

                                                                                                                                                  ttttCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAagcagc     >  1:5533773/1‑150 (MQ=255)

AAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAAGCAGCCTT  >  NC_002947/5084277‑5084575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`